Potential use of oil palm sap on lactic acid production and product adsorption on Dowex™ 66 resin as adsorbent

Abstract

Lactic acid is a product that finds several applications in food, cosmetic, pharmaceutical, and chemical industries. The main objective of this work is to evaluate potential use of the oil palm (Elaeis guineensis) sap as a substrate by Lactobacillus casei. The effects of pH control and nutrient supplement of oil palm sap on fermentation performance were investigated. In this report, when oil palm sap was used as a carbon source for L. casei, pH control did not significantly affect lactic acid production. The addition of de Man, Rogosa, and Sharpe medium improved the biomass and the product yield for which the lactic acid production in static flask at 37 C and pH 5.5 using 20 g L À1 of total sugar was improved to 0.55 g L À1 h À1 . Oil palm sap could serve as a good potential source of raw materials for efficient production of lactic acid by L. casei. When lactic acid was separated from the fermentation broth with Dowex ™ 66 resin by water as eluant, the desorption yield was 75.16%.

Pressed sap from oil palm (Elaeis guineensis) trunks: a revolutionary growth medium for the biotechnological industry?

“…56 Lactobacillus casei was used to produce 22 g L −1 of lactic acid from the palm sap (20 g L −1 total sugar). 78 With the Bacillus coagulans strain 191, a maximum yield of 75.1 g L −1 was achieved from an initial sugar concentration of 141 g L −1 (containing 67.5 g L −1 glucose). 56 Even though the highest g L −1 yield was achieved here, the total sugar amount was not totally metabolized.…”

“…The fermentative transformation of the sap from the oil palm trunk to ethanol or lactic acid, as well as other products, has already been investigated successively, which confirms that the pressed sap from the oil palm trunk is a suitable growth medium for microorganisms (Table 3). 22,51,74,75,78 …”

“…An advantage of the Lactobacillus strain seems that it grows on oil palm sap without the addition of nutrients but the highest product yield was achieved with Bacillus coagulans strain 191 56 . Lactobacillus casei was used to produce 22 g L −1 of lactic acid from the palm sap (20 g L −1 total sugar) 78 . With the Bacillus coagulans strain 191, a maximum yield of 75.1 g L −1 was achieved from an initial sugar concentration of 141 g L −1 (containing 67.5 g L −1 glucose) 56 .…”

“…71 The fermentative transformation of the sap from the oil palm trunk to ethanol or lactic acid, as well as other products, has already been investigated successively, which confirms that the pressed sap from the oil palm trunk is a suitable growth medium for microorganisms (Table 3). 22,51,74,75,78 For the production of ethanol different microorganisms have been applied, such as Saccharomyces cerevisiae strains, Zymobacter palmae strains, and a Pichia stipitis strain. 22,51,72 The best product yields have been achieved with Saccharomyces cerevisiae, so far.…”

Enzymatic Saccharification on Ammonia Pre-Treated Oil Palm Trunk Biomass for Glucose Production: An Optimization Using Response Surface Methodology

“…Oil palm (Elaeis guineensis) is broadly established in tropical countries such as Malaysia, Indonesia, and Thailand for its comestible oil [1]. In general, the oil palm starts bearing its oil in 2.5 years after being planted, and become…”