Potential Utility of Sodium Selenate as an Adjunct to Metformin in Treating Type II Diabetes Mellitus in Rats: A Perspective on Protein Tyrosine Phosphatase

Salama, Rania M.; Schaalan, Mona F.; Elkoussi, Alaaeldin A.; Khalifa, Amani E.

doi:10.1155/2013/231378

Cited by 12 publications

(11 citation statements)

References 49 publications

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“…At the first steps, diabetes induction by STZ caused rapid reduction in body weight. This was in agreement with previous reports [30]. The body weight loss in diabetic rats could be explained by many reasons, including dehydration as well as excessive fats and proteins catabolism [31], which ultimately leads to muscle wasting [32].…”

Section: Discussionsupporting

confidence: 93%

Propolis restored adiponectin level in type 2 diabetes through PPARγ activation

Elissa

Elsherbiny²,

Magmomah³

2015

Egyptian Journal of Basic and Applied Sciences

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Available online A B S T R A C TAdipose tissue regulates insulin sensitivity via the circulating adipocytokines, leptin, resistin and adiponectin. Hypoadiponectinemia contributes to the development of obesity and related disorders such as diabetes, hyperlipidemia, and cardiovascular diseases. In this study, we investigated the effects of Brazilian propolis on adiponectin levels in type 2 diabetes mellitus (T2DM), the mechanism of signaling pathway was explored as well. T2DM was induced in male Wistar rats using high fat diet and low dose of streptozotocin (STZ, 35 mg/kg, i.p.).Propolis was administered by oral tubes. Peroxisome proliferator activated receptor gamma (PPARγ) levels in sub abdominal adipose tissue, serum levels of adiponectin, tumor necrosis factor-α (TNF-α) and insulin were detected by Enzyme Linked Immunosorbent Assay (ELISA).Malondialdehyde (MDA) and reduced glutathione (GSH) in sub abdominal adipose tissue, fasting plasma glucose, plasma triglycerides and total cholesterol levels were measured by colorimetric method. Results showed that Brazilian propolis ameliorated hypoadiponectinemia in T2DM rats and relieved high glucose-induced adiponectin decrease. The signaling pathway analysis indicated that PPARγ regulation was involved. In conclusion, Brazilian propolis could have beneficial effect in T2DM by increasing tissue PPARγ levels, restoring serum adiponectin levels, enhancing insulin sensitivity and subsequently, attenuating elevated glucose level.

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Section: Discussionsupporting

confidence: 93%

Propolis restored adiponectin level in type 2 diabetes through PPARγ activation

Elissa

Elsherbiny²,

Magmomah³

2015

Egyptian Journal of Basic and Applied Sciences

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“…To determine insulin sensitivity, blood was collected 30 min after oral glucose load, and blood glucose and insulin concentrations were measured simultaneously (ELISA kit for insulin assay). Homeostasis model assessment of the insulin resistance (HOMA-IR) index was calculated according to the following formula: HOMA-IR index = blood glucose (mmol/ L) × blood insulin (mIU/L)/22.5 [36].…”

Section: Glucose and Insulin Tolerance Test In Micementioning

confidence: 99%

Nandinine, a Derivative of Berberine, Inhibits Inflammation and Reduces Insulin Resistance in Adipocytes via Regulation of AMP-Kinase Activity

Zhao

Liu

et al. 2016

Planta Med

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Nandinine is a derivative of berberine that has high efficacy for treating cardiovascular diseases. This study investigated the effects of berberine and nandinine on the regulation of insulin sensitivity in adipocytes. Through treatment with macrophage-derived conditioned medium in 3T3-L1 adipocytes, dysregulation of adipokine production and activation of the IB kinase /nuclear factor-kappa B pathway was induced. However, these phenomena were effectively reversed by berberine, nandinine, and salicylate pretreatments. Furthermore, both berberine and nandinine inhibited serine phosphorylation of insulin receptor substrate-1 induced by IB kinase and increased tyrosine phosphorylation of insulin receptor substrate-1 to activate the PI3K/Akt pathway, which finally led to insulin-mediated glucose uptake. In addition, berberine and nandinine significantly increased AMP-activated protein kinase activity, thereby contributing to their anti-inflammatory effect by inhibiting IB kinase activation. Finally, studies demonstrated that both berberine (100 or 200 mg/kg) and nandinine (100 or 200 mg/kg) effectively ameliorated glucose intolerance and induced the insulin sensitivity index in mice. In conclusion, berberine and nandinine attenuated insulin resistance in adipocytes by inhibiting inflammation in an AMP-activated protein kinase-dependent manner. Berberine and nandinine may be used as dietary supplements and nandinine is a new candidate for obesity treatment.

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“…In order to determine insulin resistance, blood was collected at 30 min after the glucose load for the assessment of insulin (with ELISA kit) and glucose. Homeostasis model assessment of the insulin resistance (HOMA-IR) index was calculated according to the formula: HOMA-IR index = blood glucose (mmol/L) × blood insulin (mIU/L)/22.5 [17].…”

Section: Oral Glucose Load Test and Insulin Sensitivity Assay In Micementioning

confidence: 99%

The Total Phenolic Fraction of Anemarrhena asphodeloides Inhibits Inflammation and Reduces Insulin Resistance in Adipocytes via Regulation of AMP-Kinase Activity

et al. 2014

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Anemarrhena asphodeloides is widely used for treatment of metabolic disorders in traditional Chinese medicine. This study investigated the effects of the total phenolic fraction of Anemarrhena asphodeloides on regulation of insulin sensitivity in adipocytes. Through treatment with macrophage-derived conditioned medium, insulin resistance was induced in adipocytes with IKKβ activation and dysregulation of adipokine production. However, these changes were reversed by treatment with the total phenolic fraction of A. asphodeloides (1, 10, 50 µg/mL). It regulated serine/tyrosine phosphorylation of insulin receptor substrate-1 and subsequently restored Akt phosphorylation in response to insulin, thereby leading to the improvement of insulin-mediated glucose uptake. The total phenolic fraction of A. asphodeloides enhanced AMP-activated protein kinase phosphorylation, and this action contributed to the inhibition of inflammation implicated in insulin resistance. In conclusion, the total phenolic fraction of A. asphodeloides attenuated insulin resistance in adipocytes by inhibition of inflammation in an AMP-activated protein kinase-dependent manner.

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Potential Utility of Sodium Selenate as an Adjunct to Metformin in Treating Type II Diabetes Mellitus in Rats: A Perspective on Protein Tyrosine Phosphatase

Cited by 12 publications

References 49 publications

Propolis restored adiponectin level in type 2 diabetes through PPARγ activation

Propolis restored adiponectin level in type 2 diabetes through PPARγ activation

Nandinine, a Derivative of Berberine, Inhibits Inflammation and Reduces Insulin Resistance in Adipocytes via Regulation of AMP-Kinase Activity

The Total Phenolic Fraction of Anemarrhena asphodeloides Inhibits Inflammation and Reduces Insulin Resistance in Adipocytes via Regulation of AMP-Kinase Activity

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