Potentiating response of D- Ribose-L-Cysteine on Sodium arsenate- induced hormonal imbalance, spermatogenesis impairments and histomorphometric alterations in adult male Wistar rat

Abstract

Objective: Reproductive toxicity is an important health challenge, mostly associated with exposure to several environmental toxicants. Arsenic is a ubiquitous toxic compound naturally present in the environment. This study was carried out to evaluate the dietary supplements of D-Ribose-L-Cysteine against sodium arsenate-induced testicular toxicity in adult male Wistar rats.Methods: A total of 32 male rats (150-250g) were randomly divided into four ( 4) groups (n=8). Group A received normal saline as placebo; Group B received 8mg/ kg BW of Sodium arsenate only; Group C received 8mg/ kg BW of Sodium arsenate and 10 mg/kg BW of D-Ribose-L-cysteine; Group D received 8mg/kg BW of Sodium arsenate and 30 mg/kg BW of D-Ribose-L-cysteine. All administration was done via oral gavage for 28 days, thereafter the animals were sedated with pentobarbital sodium (intraperitoneally); we obtained testes and blood serum for analysis.Results: The results showed abnormal testicular morphology with degeneration and decrease in spermatogonia, vacuolation and empty lumen, intense necrosis, spermatogenesis disruption (decrease sperm count, motility, viability) and degraded germinal epithelium of the seminiferous tubules, reduction in the hormone profile (FSH, LH, and TT) and oxidative stress parameters (CAT, GSH, and SOD) with a corresponding increase in MDA level in the arsenic-only treated rats (group B) compared to their control counterparts (group A), but it was ameliorated after DRLC administration, both in low and high doses, respectively.Conclusions: D-Ribose-L-Cysteine attenuated distorted testicular morphology, altered semen characteristics, hormone profile, and oxidative stress markers by preventing the deleterious toxicity of sodium arsenate.

Contemporary Comprehensive Review on Arsenic-Induced Male Reproductive Toxicity and Mechanisms of Phytonutrient Intervention

“…Treatment with 200 mg/kg Vitamin E for 84 days increased spermatogenesis and restored blood LH, FSH, and testosterone levels [167], and improved semen quality in Teddy goat buck testicular tissue [200]. Oral administration of D-ribose-L-Cysteine at doses of 10 and 30 mg/kg over 28 days restored sperm count, motility, viability, LH, FSH, testosterone, and CAT, SOD, and GSH activities in rat testes [197]. In a recent study, nano vitamin C (NVC) was found to ameliorate arsenic-induced changes in testicular and sperm parameters.…”

Therapeutic effects of aqueous extract of bioactive active component of Ageratum conyzoides on the ovarian-uterine and hypophysis-gonadal axis in rat with polycystic ovary syndrome: Histomorphometric evaluation and biochemical assessment

“…Histological analyses of the ovary and the uterus were assessed from 5 μm sections of paraffin-embedded tissues following hematoxylin-eosin staining [ [61] , [62] , [63] ]. Histomorphological changes were assessed on microphotographs using a Scientico STM-50 microscope equipped with a Celestron MA411101 camera connected to a computer where the image was transferred and analyzed with the Image J1.3 software.…”

Histomorphological response of D-ribose L-cysteine to ketamine-induced testicular toxicity in adult male Wistar rats

“…The hormonal levels of Testosterone (TT), Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) were measured using available immunoassay (ELISA) kits (Randox Laboratories Ltd, Admore Diamond Road, Crumlin, Co., Antrim, United Kingdom, Qt94QY) according to manufacturer instructions, following the method of Ogunlade et al (2021).…”