Potentiation between Pentagastrin and Bethanechol Chloride in Stimulation of Gastric Secretion from Isolated &lt;i&gt;ex vivo&lt;/i&gt; Canine Stomach Perfused with Homologous Blood

Kowalewski, K.; Saab, Miriam Wissam

doi:10.1159/000136293

Cited by 12 publications

(19 citation statements)

References 7 publications

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“…It (16,(21)(22)(23)(24)(25); however, interactions demonstrated in this manner are species dependent and variable in magnitude (15,(26)(27)(28). A dramatic demonstration of secretagogue interactions has been made in studies with the ex vivo canine stomach preparation where marked potentiation was found between histamine and pentagastrin, between histamine and bethanechol, and between pentagastrin and bethanechol (29,30). Further in vivo evidence for interactions between secretagogues comes from studies of secretagogue effect on acid secretion after both vagotomy and antrectomy.…”

Section: Discussionmentioning

confidence: 99%

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

Soll¹

1978

J. Clin. Invest.

164

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A B ST RA CT Using oxygen uptake as an index of the physiological response of isolated parietal cells, the interactions between histamine and gastrin and between histamine and carbamylcholine and the effects of atropine and metiamide on these interactions have been studied. Parietal cells were isolated from canine fundic mucosa by sequential exposure of separated mucosa to collagenase and EDTA. In previous studies carbamylcholine, isobutyl methyl xanthine, gastrin, and histamine have each been shown to increase oxygen uptake by these cells. Isobutyl methyl xanthine greatly enhanced the histamine effect. Carbamylcholine was inhibited by atropine but not by metiamide, histamine was inhibited by metiamide but not by atropine, and gastrin was inhibited by neither, suggesting that each of these agents has a direct action on the parietal cell. In the present studies, potentiating interactions between histamine and carbamylcholine and between histamine and gastrin have been demonstrated. Against a histamine (0.1 and 1 ,M) plus isobutyl methyl xanthine (0.1 mM) background, the dose for 50% response for gastrin was approximately 1 nM, and the maximal response was obtained at 0.1 AM.When added to these combinations of stimulants, metiamide and atropine retained their respective specificities against stimulation by histamine and carbamylcholine, in that responses were inhibited to the level that was seen when the component of the pair that was not inhibited was given alone.The observation that histamine plus gastrin andThis work was presented, in part, at the meeting of the

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Section: Discussionmentioning

confidence: 99%

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

Soll¹

1978

J. Clin. Invest.

164

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“…Mechanical activity was monitored through a strain gauge which was stitched over the antrum. Figure 3 shows the positioning of electrodes and strain gauge on the stomach [1,2,14,18,19]. When stimulation of the vagus was per formed, a pair of platinum electrodes embedded in plastic were attached to the nerve of Latarjet on the lesser curvature.…”

Section: Methodsmentioning

confidence: 99%

Effect of Ischemic Anoxia on Electrical and Mechanical Activity of the Totally Isolated Porcine Stomach

Kowalewski¹,

Zajac²,

Kolodej³

1976

Eur Surg Res

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Electrical and mechanical activity were recorded from totally isolated whole porcine stomachs perfused with homologous blood. Stagnant, complete ischemia was then produced by closing the gastric artery and vein for various periods of time up to 3 h. After a given period of ischemia, blood was recirculated for 10 min and then pentagastrin was injected into the gastric artery. The vagus nerve was also electrically stimulated at various stages of anoxia and recirculation. After up to 90 min of ischemia, stomachs were able to demonstrate electrical control activity of low frequency after 10 min of blood recirculation. After injection of pentagastrin, electrical control activity rapidly became normal. When ischemia lasted 120 min or longer, recirculation of blood did not induce reappearance of electrical control activity, but pentagastrin injection produced a normal response. After ischemia of 240 min duration or longer, recirculation and pentagastrin had no effect. In previously ischemic stomachs the response to vagal stimulation occurred only in the stomachs responding to pentagastrin.

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“…Another method is to establish first the maximal dose of each agent separately, and then use both stimulants at maximal dosage simultaneously [14]. We tested the following doses of pentagastrin: 2, 8, 16, 32 and 64 /<g/h in several ex vivo stomachs [10]. This permitted us to establish that the highest output of HC1 was obtained with a dose of 16 ug/h of pentagastrin.…”

Section: Measurementsmentioning

confidence: 99%

Potentiation between Pentagastrin and Histamine in Stimulation of Secretion from Totally Isolated <i>ex vivo</i> Canine Stomach

Kowalewski¹,

Kolodej²

1972

Pharmacology

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Totally isolated canine stomachs, perfused with homologous blood, were used for the study of potentiation between pentagastrin and histamine. These stimulants were infused into the gastric arterial system separately, or combined. Gastric secretion and perfusion blood were sampled every 30 min for biochemical analyses Combination of pentagastrin plus histamine gave significantly higher outputs of HCl than could be accounted for on the basis of summation of stimulatory effects. True potentiation, with regard to gastric juice volume and HCl output, occurred when two stimuli were infused simultaneously. Gastric hypersecretion of H⁺ was associated with increased O₂^–consumption by the stomach and by the alteration of the physiological relationship between the pH of arterial and venous blood sampled from gastric vessels. It was concluded that the totally isolated canine ex vivo stomach, perfused with homologous blood, is a suitable experimental preparation for the study of potentiation effects between two humoral gastric secretagogues.

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Potentiation between Pentagastrin and Bethanechol Chloride in Stimulation of Gastric Secretion from Isolated <i>ex vivo</i> Canine Stomach Perfused with Homologous Blood

Cited by 12 publications

References 7 publications

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

The Interaction of Histamine with Gastrin and Carbamylcholine on Oxygen Uptake by Isolated Mammalian Parietal Cells

Effect of Ischemic Anoxia on Electrical and Mechanical Activity of the Totally Isolated Porcine Stomach

Potentiation between Pentagastrin and Histamine in Stimulation of Secretion from Totally Isolated <i>ex vivo</i> Canine Stomach

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