Potentiation of cell-mediated immune responses against recombinant HN protein of Newcastle disease virus by recombinant chicken IL-18

Su, Bor Sheu; Shen, Pin Chun; Hung, Li Hsiang; Huang, Ji Ping; Yin, Hao; Lee, Long Huw

doi:10.1016/j.vetimm.2011.03.018

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…Our present findings are also supported by our recently published data that chIL-18 combined with chIFN-α had enhanced antiviral and immunomodulatory properties against AIV H9N2 in vivo when administered orally using attenuated S. enterica serovar Typhimurium strain χ8501 (13). Furthermore, recently it is reported that recombinant chIL-18 potentiated both the humoral and cell mediated immune responses against recombinant Newcastle disease virus vaccine (21,22) which directly supports our findings. The use of attenuated S. enterica serovar Typhimurium strain χ8501 as a successful mammalian and avian cytokine genes delivery system is well addressed at our laboratory in our several recent publications (13,17,23,24).…”

Section: Discussionsupporting

confidence: 91%

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Rahman

Uyangaa

2013

Immune Netw

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Interleukin-18 (IL-18) has been known to induce interferon-γ (IFN-γ) production and promote Th1 immunity. Although mammalian IL-18 has been characterized in great detail, the properties and application of chicken IL-18 remain largely uninvestigated as of yet. In this study, we evaluated the immunomodulatory properties of Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 (chIL-18) on immune responses induced by avian influenza (AI) and Newcastle disease (ND) vaccines. After oral administration of S. enterica serovar Typhimurium expressing chIL-18, chickens were vaccinated intramuscularly with the recommended dose of either inactivated AI H9N2 vaccine or ND (B1 strain) vaccine. Chickens receiving a primary vaccination were boosted using the same protocol 7 days later. Humoral and cell-mediated immune responses were evaluated in terms of HI antibody titers and proliferation and mRNA expression of IFN-γ and IL-4 of peripheral blood mononuclear cells (PBMC) in response to specific antigen stimulation. According to our results, oral administration of S. enterica serovar Typhimurium expressing chIL-18 induced enhanced humoral and Th1-biased cell-mediated immunity against AI and ND vaccines, compared to that of chickens received S. enterica serovar Typhimurium harboring empty vector. Therefore, we conclude that our proposed vaccination regimen using inactivated AI and ND viruses along with oral administration of S. enterica serovar Typhimurium expressing chIL-18 may provide a novel approach in protecting chicken from currently circulating AI and ND virus strains.

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Section: Discussionsupporting

confidence: 91%

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Rahman

Uyangaa

2013

Immune Netw

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“…IL-18 is a strong IFN-␥-inducing factor that enhances immune responses when used as an adjuvant. The concept of using IL-18 to boost immune responses after vaccination has been investigated for chicken vaccines (23,24,32,(36)(37)(38). Hung et al (21) described that the full-length and mature chicken IL-18 purified recombinant protein effectively enhanced cell-mediated and humoral immunity.…”

Section: Discussionmentioning

confidence: 99%

“…Hung et al (21) confirmed that chicken IL-18 recombinant proteins exert in vivo biological functions through stimulating humoral and cellmediated immunities in order to enhance antigen-specific immunity and vaccine efficacy. Although many studies have shown that recombinant ChIL-18 boosts the immune responses to avian virus vaccines (22)(23)(24), few studies have investigated the modulatory effect of using a eukaryotic expression plasmid carrying ChIL-18 as a molecular genetic adjuvant to enhance these vaccines. In this study, we cloned the full-length ChIL-18 gene from specificpathogen-free (SPF) chicken embryo spleen cells and report on a eukaryotic expression plasmid carrying ChIL-18 as a genetic adjuvant, coadministered with an inactivated NDV vaccine, which induced strong immune responses in chickens at both the humoral and cellular levels.…”

mentioning

confidence: 99%

A Eukaryotic Expression Plasmid Carrying Chicken Interleukin-18 Enhances the Response to Newcastle Disease Virus Vaccine

Wang¹,

Li²,

Zhang³

et al. 2014

Clin. Vaccine Immunol.

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Interleukin-18 (IL-18) is an important cytokine involved in innate and acquired immunity. In this study, we cloned the fulllength chicken IL-18 (ChIL-18) gene from specific-pathogen-free (SPF) chicken embryo spleen cells and provided evidence that the ChIL-18 gene in a recombinant plasmid was successfully expressed in chicken DT40 cells. ChIL-18 significantly enhanced gamma interferon (IFN-␥) mRNA expression in chicken splenocytes, which increased IFN-␥-induced nitric oxide (NO) synthesis by macrophages. The potential genetic adjuvant activity of the ChIL-18 plasmid was examined in chickens by coinjecting ChIL-18 plasmid and inactivated Newcastle disease virus (NDV) vaccine. ChIL-18 markedly elevated serum hemagglutination inhibition (HI) titers and anti-hemagglutinin-neuraminidase (anti-HN)-specific antibody levels, induced the secretion of both Th1-(IFN-␥) and Th2-(interleukin-4) type cytokines, promoted the proliferation of T and B lymphocytes, and increased the populations of CD3؉ T cells and their subsets, CD3 ؉ CD4 ؉ and CD3 ؉ CD8 ؉ T cells. Furthermore, a virus challenge revealed that ChIL-18 contributed to protection against Newcastle disease virus challenge. Taken together, our data indicate that the coadministration of ChIL-18 plasmid and NDV vaccine induces a strong immune response at both the humoral and cellular levels and that ChIL-18 is a novel immunoadjuvant suitable for NDV vaccination. N ewcastle disease (ND) is a serious avian disease that causes substantial economic loss and remains a major threat to the poultry industry (1, 2). Outbreaks of ND among poultry occur worldwide, and the pathogenic form of the virus is a disease listed in the World Organization for Animal Health (OIE) Terrestrial Animal Health Code and must be reported to the OIE (3), which results in severe trade limitations (4, 5). Currently, vaccination is the major tool for controlling infection by Newcastle disease virus (NDV). The NDV vaccine strains LaSota, B1, Mukteswar, and V4 are used widely in China. However, virulent NDV strains are still frequently isolated in vaccinated birds, indicating that NDV remains an ongoing threat to commercial flocks of birds (6). Therefore, it is necessary to develop more efficacious vaccines to prevent NDV infection.Many techniques have been developed to increase the immunogenicity of vaccines. Among these, cytokines are effective immunomodulators in animal models or in clinical testing (7-10). Among the large number of cytokines, interleukin-18 (IL-18) is a strong stimulator of T helper type 1 (Th1) responses and activates natural killer (NK) cells, stimulates the synthesis of other immunoactive cytokines from Th1 cells, monocytes, and NK cells, and synergizes with IL-12 in the maturation of Th1 cells and the suppression of IgE synthesis by B cells (11-13). Thus, IL-18 functions as an adjuvant (14, 15). However, depending on the cytokine environment, IL-18 may also promote Th2-type responses (16,17) and antibody formation (18).The isolation and characterization of chicken IL-18 (ChIL-18) ...

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“…Many studies have shown that recombinant ChIL-18 boosts the immune responses to avian virus vaccines [Degen et al, 2005;Chen et al, 2010;Rahman et al, 2013], and in vitro studies have recently shown that, similar to IL-1 [Degen et al, 2005], IL-18 overexpression inhibits hepatitis B virus replication in a hepatoma cell line [Chen et al, 2010]. IL-18 is also required for the activation of NK cells in response to infections with vaccinia virus and murine cytomegalovirus and plays a critical role in antiviral defense [Su et al, 2011]. IL-18 may also play an important role in the initiation of innate immune responses [Thompson et al, 2009;Zhang et al, 2011;Brandstadter et al, 2014] by mediating the severity of infections and inflammatory diseases [Meldrum et al, 2012].…”

mentioning

confidence: 99%

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

Song

Li²,

Ma³

et al. 2017

Cytogenet Genome Res

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Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified. To determine their antiviral activities, 200 ng/mL of ChIL-18 and/or ChIFN-γ were inoculated into chicken embryonic fibroblast cells. After 24 h, one 50% tissue culture infective dose (TCID₅₀) of infectious bursal disease virus (IBDV) was inoculated into the chicken embryonic fibroblast cells. The results showed that the antiviral effect of ChIL-18 and ChIFN-γ in combination was better than that of ChIL-18 or ChIFN-γ alone. Next, 14-day-old chicken were injected with 200 µg of ChIL-18 and/or ChIFN-γ and then were challenged with 10³ TCID₅₀ of IBDV via intraperitoneal injection. The results showed that the proliferation of IBDV was inhibited by the injection of the recombinant proteins, especially the combination of ChIL-18 and ChIFN-γ, as evidenced by cytokine detection, quantitative PCR, and pathology analyses. These results indicate that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL-18 and ChIFN-γ has a better inhibitory effect than either cytokine alone.

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Potentiation of cell-mediated immune responses against recombinant HN protein of Newcastle disease virus by recombinant chicken IL-18

Cited by 12 publications

References 26 publications

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

A Eukaryotic Expression Plasmid Carrying Chicken Interleukin-18 Enhances the Response to Newcastle Disease Virus Vaccine

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

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Potentiation of cell-mediated immune responses against recombinant HN protein of Newcastle disease virus by recombinant chicken IL-18

Cited by 12 publications

References 26 publications

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

A Eukaryotic Expression Plasmid Carrying Chicken Interleukin-18 Enhances the Response to Newcastle Disease Virus Vaccine

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-&#x03B3;

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Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ