Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18)

Degen, W.G.J.; Zuilekom, Hanneke I. van; Scholtes, N.; Daal, Nancy van; Schijns, Virgil E.J.C.

doi:10.1016/j.vaccine.2005.04.003

Cited by 47 publications

(19 citation statements)

References 29 publications

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“…Bacterially expressed chIL-18 is capable of inducing both the synthesis of chicken IFN-γ in cultured primary chicken spleen cells and the proliferation of CD4 + T cells (19,20). In addition, the purified Escherichia coli -expressed recombinant chIL-18 significantly enhanced antibody responses to Clostridium perfringens α-toxoid and Newcastle disease (ND) virus antigens (21). According to the above mentioned findings it is conceivable that oral administration of Salmonella enterica serovar Typhimurium expressing chIL-18 might modulate both humoral and Th1-biased cell-mediated immunity against avian influenza and Newcastle disease vaccines.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Rahman

Uyangaa

2013

Immune Netw

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Interleukin-18 (IL-18) has been known to induce interferon-γ (IFN-γ) production and promote Th1 immunity. Although mammalian IL-18 has been characterized in great detail, the properties and application of chicken IL-18 remain largely uninvestigated as of yet. In this study, we evaluated the immunomodulatory properties of Salmonella enterica serovar Typhimurium expressing chicken interleukin-18 (chIL-18) on immune responses induced by avian influenza (AI) and Newcastle disease (ND) vaccines. After oral administration of S. enterica serovar Typhimurium expressing chIL-18, chickens were vaccinated intramuscularly with the recommended dose of either inactivated AI H9N2 vaccine or ND (B1 strain) vaccine. Chickens receiving a primary vaccination were boosted using the same protocol 7 days later. Humoral and cell-mediated immune responses were evaluated in terms of HI antibody titers and proliferation and mRNA expression of IFN-γ and IL-4 of peripheral blood mononuclear cells (PBMC) in response to specific antigen stimulation. According to our results, oral administration of S. enterica serovar Typhimurium expressing chIL-18 induced enhanced humoral and Th1-biased cell-mediated immunity against AI and ND vaccines, compared to that of chickens received S. enterica serovar Typhimurium harboring empty vector. Therefore, we conclude that our proposed vaccination regimen using inactivated AI and ND viruses along with oral administration of S. enterica serovar Typhimurium expressing chIL-18 may provide a novel approach in protecting chicken from currently circulating AI and ND virus strains.

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Section: Discussionmentioning

confidence: 99%

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Rahman

Uyangaa

2013

Immune Netw

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“…Several reports have shown that the plasmid encoding IL-18, which is used as an adjuvant in mammalian vaccines, promote high antibody titers [20,21]. The recombinant chicken IL-18 has been shown to significantly enhance antibody responses to the Clostridium perfringens α-toxoid and NDV antigens [22]. Higher HI titers induced in chickens immunized with the Newcastle disease vaccine co-administrated with chicken IL-18 further confirm the immunostimulatory activities of chicken IL-18 in vivo [23].…”

Section: Discussionmentioning

confidence: 99%

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

et al. 2012

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BackgroundDNA vaccines offer several advantages over conventional vaccines in the development of effective vaccines against avian influenza virus (AIV). However, one of the limitations of the DNA vaccine in poultry is that it induces poor immune responses. In this study, chicken interleukin (IL) -15 and IL-18 were used as genetic adjuvants to improve the immune responses induced from the H5 DNA vaccination in chickens. The immunogenicity of the recombinant plasmid DNA was analyzed based on the antibody production, T cell responses and cytokine production, following inoculation in 1-day-old (Trial 1) and 14-day-old (Trial 2) specific-pathogen-free chickens. Hence, the purpose of the present study was to explore the role of chicken IL-15 and IL-18 as adjuvants following the vaccination of chickens with the H5 DNA vaccine.ResultsThe overall HI antibody titer in chickens immunized with pDis/H5 + pDis/IL-15 was higher compared to chickens immunized with pDis/H5 (p < 0.05). The findings revealed that the inoculation of the 14-day-old chickens exhibited a shorter time to achieve the highest HI titer in comparison to the inoculation of the 1-day-old chickens. The cellular immunity was assessed by the flow cytometry analysis to enumerate CD4+ and CD8 + T cells in the peripheral blood. The chickens inoculated with pDis/H5 + pDis/IL-15 demonstrated the highest increase in CD4+ T cells population relative to the control chickens. However, this study revealed that pDis/H5 + pDis/IL-15 was not significant (P > 0.05) in inducing CD8+ T cells. Meanwhile, with the exception of Trial 1, the flow cytometry results for Trial 2 demonstrated that the pDis/H5 + pDis/IL-18 inoculated group was able to trigger a higher increase in CD4+ T cells than the pDis/H5 group (P < 0.05). On the other hand, the pDis/H5 + pDis/IL-18 group was not significant (P > 0.05) in modulating CD8+ T cells population in both trials. The pDis/H5 + pDis/IL-15 inoculated group showed the highest IL-15 gene expression in both trials compared to other inoculated groups (P < 0.05). Similar results were obtained for the IL-18 expression where the pDis/H5 + pDis/IL-18 groups in both trials (Table 8) were significantly higher compared to the control group (P < 0.05). However, the expressions of other cytokines remained low or undetected by GeXP assay.ConclusionsThis study shows the diverse immunogenicity of pDis/H5 co-administered with chicken IL-15 and IL-18,with pDis/H5 + pDis/IL-15 being a better vaccine candidate compared to other groups.

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“…The report has indicated that administering ChIL-18 may be a novel approach for protecting chicken from currently circulating avian influenza and Newcastle Disease virus strains [Rahman et al, 2013]. Many studies have shown that recombinant ChIL-18 boosts the immune responses to avian virus vaccines [Degen et al, 2005;Chen et al, 2010;Rahman et al, 2013], and in vitro studies have recently shown that, similar to IL-1 [Degen et al, 2005], IL-18 overexpression inhibits hepatitis B virus replication in a hepatoma cell line [Chen et al, 2010]. IL-18 is also required for the activation of NK cells in response to infections with vaccinia virus and murine cytomegalovirus and plays a critical role in antiviral defense [Su et al, 2011].…”

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confidence: 99%

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

Song

Li²,

Ma³

et al. 2017

Cytogenet Genome Res

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Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified. To determine their antiviral activities, 200 ng/mL of ChIL-18 and/or ChIFN-γ were inoculated into chicken embryonic fibroblast cells. After 24 h, one 50% tissue culture infective dose (TCID₅₀) of infectious bursal disease virus (IBDV) was inoculated into the chicken embryonic fibroblast cells. The results showed that the antiviral effect of ChIL-18 and ChIFN-γ in combination was better than that of ChIL-18 or ChIFN-γ alone. Next, 14-day-old chicken were injected with 200 µg of ChIL-18 and/or ChIFN-γ and then were challenged with 10³ TCID₅₀ of IBDV via intraperitoneal injection. The results showed that the proliferation of IBDV was inhibited by the injection of the recombinant proteins, especially the combination of ChIL-18 and ChIFN-γ, as evidenced by cytokine detection, quantitative PCR, and pathology analyses. These results indicate that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL-18 and ChIFN-γ has a better inhibitory effect than either cytokine alone.

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Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18)

Cited by 47 publications

References 29 publications

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

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Potentiation of humoral immune responses to vaccine antigens by recombinant chicken IL-18 (rChIL-18)

Cited by 47 publications

References 29 publications

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Modulation of Humoral and Cell-Mediated Immunity Against Avian Influenza and Newcastle Disease Vaccines by Oral Administration ofSalmonella entericaSerovar Typhimurium Expressing Chicken Interleukin-18

Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responses

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-&#x03B3;

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Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ