Potentiometric coulometry based on charge accumulation with a peroxidase/osmium polymer-immobilized electrode for sensitive determination of hydrogen peroxide

Nieh, Chi-Hua; Kitazumi, Yuki; Shirai, Osamu; Yamamoto, Masahiro; Kano, Kenji

doi:10.1016/j.elecom.2013.05.007

Cited by 9 publications

(13 citation statements)

References 10 publications

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“…Recently, a technique coupling potentiometric coulometry with bioelectrocatalytic charge accumulation has been developed [10], in which the amount of charge accumulated on an immobilized redox polymer on the electrode is determined potentiometrically. The change in electrode potential corresponds to the change in the ratio of the reduced state to the oxidized state of the side chains in the polymer.…”

Section: Introductionmentioning

confidence: 99%

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Potentiometric coulometry using a liquid-film-modified electrode as a reversible surface-confined system

Katsube

Kitazumi

Shirai

et al. 2016

Journal of Electroanalytical Chemistry

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Fax: +81-75-753-6456; Tel: +81-75-753-6392 (K. Kano) A reversible surface-confined system for potentiometric coulometry studies was fabricated by the deposition of a liquid film on an electrode surface, and the system was used to detect charge accumulation due to the peroxidase (POD)-catalyzed reduction of H 2 O 2 . The components of the liquid film were 1-ethyl-3-methylimidazolium bis(nonafluorobutanesulfonyl)imide as the electrolyte, dibutyl phthalate as the medium, and ferrocene as the redox-active mediator. When the thickness of the liquid film was less than that of the diffusion layer in the minimum potential step of the apparatus, a symmetric voltammogram, according to the Nernst equation, was recorded. The reduction of H 2 O 2 by POD was carried out on the liquid-film-modified electrode and studied by potentiometric coulometry. The addition of H 2 O 2 shifts the electrode potential positively, due to the oxidation of ferrocene. The change in the potential is in agreement with the Nernst equation. Thus, potentiometric coulometry, carried out on the liquid-film-modified electrode, enables the determination of the substrate without the requirement of a calibration curve.

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Section: Introductionmentioning

confidence: 99%

“…However, the response of the redox polymer deviated from the Nernst equation because of the strong electrostatic interactions that exist between the redox sites in the polymer. Therefore, the determination of the amount of accumulated charge required a calibration curve based on the voltammogram [10].…”

Section: Introductionmentioning

confidence: 99%

Potentiometric coulometry using a liquid-film-modified electrode as a reversible surface-confined system

Katsube

Kitazumi

Shirai

et al. 2016

Journal of Electroanalytical Chemistry

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“…However, the substrate specificity for one of the two substrates is not so high for almost all redox enzymes. Therefore, the substrate may be replaced with artificial electron acceptor (or donor) in vitro, and the redox enzyme reactions can be combined with electrode reaction, when once the artificial electron acceptor (or donor) is reoxidized (or rereduced) at electrodes [3][4][5]. Furthermore, a limited number of enzymes can directly transfer electron to (or from) electrode [6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…The former and the latter are called mediated (MET) and direct electron transfer (DET)-type bioelectrocatalysis reactions, respectively. Both reactions are utilized to construct bioelectrochemical devices, such as biofuel cells and biosensors [5,7,[15][16][17]. MET-type bio-devices might be superior in part to DET-type ones in the performance, since most of enzymes show rather low DET-type catalytic activity.…”

Section: Introductionmentioning

confidence: 99%

Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase

Kawai

Yakushi

Matsushita

et al. 2015

Electrochimica Acta

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A heterotrimeric membrane-bound fructose dehydrogenase (FDH) from Gluconobacter japonicus NBRC3260 contains FAD in subunit I and three heme C moieties in subunit II as the redox centers, and is one of the direct electron transfer (DET)-type redox enzymes. FDH-catalyzed current density of fructose oxidation at hydrophilic mercaptoethanol (MEtOH)-modified Au electrode is much larger than that at hydrophobic mercaptoethane (MEtn)-modified Au electrode. Addition of a non-ionic surfactant Triton ® X-100 (1%) completely quenches the catalytic current at the MEtnmodified Au electrode, while only small competitive effect is observed at the MEtOHmodified Au electrode. Quartz crystal microbalance measurements support the adsorption of FDH and Triton ® X-100 on both of the modified electrodes. We propose a model to explain the phenomenon as follows. The surfactant forms a monolayer on the hydrophobic MEtn-modified electrode with strong hydrophobic interaction, and FDH adsorbs on the surface of the surfactant monolayer. The monolayer inhibits the electron transfer from FDH to the electrode. On the other hand, the surfactant forms a bilayer on the hydrophilic MEtOH-modified electrode. The interaction between the surfactant bilayer and the hydrophilic electrode is relatively weak so that FDH replaces the surfactant and is embedded in the bilayer to communicate electrochemically with the hydrophilic electrode.

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“…(2)(3)(4)(5)(6)(7)(8)(9) Among these electrochemical biosensors, potentiometric devices are advantageous for imaging because their sensitivity essentially does not change with decreasing sensing area. In addition, potentiometric image sensors, or pH image sensors, have been developed to visualize the two-dimensional pH distribution in solutions.…”

Section: Introductionmentioning

confidence: 99%

Development of Glutamate Sensor for Neurotransmitter Imaging

2017

Sensors and Materials

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We report an enzyme-based potentiometric glutamate sensor combined with a semiconductor device for neurotransmitter imaging, the response of which is based on the detection of the interfacial potential of a gold electrode produced by redox and enzyme reactions. First, we confirmed that the interfacial potential increased logarithmically with the increasing concentration ratio of hexacyanoferrate(III) to hexacyanoferrate(II). The proposed sensor could detect the interfacial potential change of the gold electrode with a slope of 66.1 mV/decade. H 2 O 2 and l-glutamate were detected as a function of the change in the interfacial potential of the gold electrode in the range of 0.01-1 mM. Slopes of 55.2 and 41.9 mV/decade were obtained as the sensitivities for H 2 O 2 and l-glutamate, respectively. These estimated values are larger than the theoretical value of 29.5 mV/decade. Although the reason why the difference between the estimated value and the theoretical value occurred could not be revealed, the interfacial potential is expected to depend on some kind of reaction related to H 2 O 2 concentration because the logarithmic response was obtained for H 2 O 2 concentration. Therefore, the results suggested that the sensor can detect the l-glutamate concentration.

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Potentiometric coulometry based on charge accumulation with a peroxidase/osmium polymer-immobilized electrode for sensitive determination of hydrogen peroxide

Cited by 9 publications

References 10 publications

Potentiometric coulometry using a liquid-film-modified electrode as a reversible surface-confined system

Potentiometric coulometry using a liquid-film-modified electrode as a reversible surface-confined system

Role of a non-ionic surfactant in direct electron transfer-type bioelectrocatalysis by fructose dehydrogenase

Development of Glutamate Sensor for Neurotransmitter Imaging

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