Potentiometric determination of mepivacaine hydrochloride local anesthetic drug in pharmaceutical and biological fluids using ion selective electrode

Abstract

The objective of this work is to determine mepivacaine hydrochloride (MPVHC) as local anesthetic drug. Modified carbon paste ion-selective electrode was used for the determination of MPVHC in different dosage forms and biological fluids. The study depends on potentiometric titration of MPVHC drug using modified carbon paste (MCPE) as end point indicator electrode. The effects of the paste composition, different conditioning parameters and foreign ions on the electrode performance were investigated and response time of the electrode has been studied. The results obtained showed that carbon paste electrode modified with sodium tetraphenyl borate-MPV ion pair gives the highest potential break at the end point, Nernstian slope, wide concentration range and lower detection limit than the reinckate-, phosphotungestic-and phosphomolybdic-MPV ion pairs. The fabricated electrode obtained Nernstian response of 57.71±0.67 mV decade -1 in the concentration range of 3.1×10 -7 -1.0×10 -1 mol L -1 for MCPE electrode. The electrode was found to be operating within the pH range of 3.0-8.0 and exhibited a fast response time (about 14 s), low detection limit of 3.1×10 -7 mol L -1 and long lifetime (98 days). The electrode showed high selectivity to the MPVHC drug in the presence of different organic, inorganic and amino acids. The electrode was successfully applied for the determination of MPVHC in pharmaceutical preparations and biological fluids (urine and plasma) with high percent recovery and low standard and relative standard deviation values. The results obtained applying this potentiometric electrode are comparable with British pharmacopeia. The method validation parameters were optimized and the method can be applied for routine analysis of MPVHC drug.

Modified Screen-Printed Microchip for Potentiometric Detection of Terbinafine Drugs

“…It is the relative response of the proposed sensor for principal ions over other interfering ions present in solution. Terefore, the potentiometric selectivity coefcient of the terbinafne microchip was determined using a separate solution method (SSM) [14] by separate calibration for terbinafne as well as all studied interfering ions in the concentration range of 10 −6 -10 −2 , as presented in Figure 7. Te values of the selectivity coefcient for all studied interfering species were calculated, and the results obtained are summarized in Table 2.…”

“…Te performance response parameters of the realized terbinafne microchip assembly in comparison with the bulk electrodes published are summarized in Table 3. It should be noted that the performance properties (slope, detection limit, and linear range) of the chip are better than those reported for terbinafne bulk electrodes [14,15]. Tis behavior is attributed to the incorporation of the MWCNTs into the sensitive element, which improves the conductivity of the sensor, increases the transduction of the chemical signal to the electrical signal, and therefore increases the sensitivity of electrodes [19,21].…”

“…Other than ofcial methods, titration with perchloric acid in acetic acid media [1] and diferent chromatographic techniques [1][2][3][4][5][6][7][8][9][10] have also been reported for the determination of terbinafne. Spectrophotometric methods (UV-visible) [1,11,12], spectrofuorimetric [1,13], electroanalytical methods [1,14,15], capillary electrophoresis [1,16], and cylinderplate based microbiological assay [1,17] have also been reported for terbinafne determination.…”

Spectrophotometric/Titrimetric Drug Analysis

“…It is noted from the table that spectroscopic and titration methods are still widely used in the pharmaceutical analysis. To name a few, in the literature survey, titrimetric methods have been used for the determination of terbinafine [4][5][6][7][8], ethionamide [9][10][11][12][13], and Amoxicillin [14 -20] in pharmaceutical formulations. In addition to many drug formulations that were estimated using titration methods.…”