Potentiometric Enzyme Methods

Kobos, R. K.

doi:10.1007/978-1-4684-3776-8_1

Cited by 12 publications

(6 citation statements)

References 197 publications

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“…The development of enzyme-, bacterial-, and tissue-based biomembrane electrodes selective for physiologically important compounds continues to be an expanding and exciting area of research (1)(2)(3)(4)(5). To be of practical analytical utility, such electrodes must possess certain desirable response characteristics including high selectivity, rapid response times, freedom from inhibitor interferences which may be present in real samples, and long-term stability.…”

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confidence: 99%

Development and application of a histidine-selective biomembrane electrode

Kovach¹,

Meyerhoff²

1982

Anal. Chem.

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A highly selective histidine biomembrane electrode has been prepared by Immobilizing the enzyme histidine decarboxylase (E.C. No. 4.1.1.22) at the surface of a potentiometric carbon dioxide sensor. The enzyme employed was extracted from Lactobacillus 30a. The use of concentrated enzyme extract rather than Intact bacterial cells Is shown to yield bloelectrodes with Improved response characteristics. The resulting enzyme-based sensor responds linearly to the logarithm of ¿.-histidine concentration between 3 X 10~4 and 1 X 10~2 mol/L with a slope typically of 48-53 mV/decade and a useful

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confidence: 99%

Development and application of a histidine-selective biomembrane electrode

Kovach¹,

Meyerhoff²

1982

Anal. Chem.

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“…Noncharged organic substances such as DOP and TCE were detected satisfactorily using a potentiometric measurement method. In potentiometric enzyme sensors, products such as H and O are usually measured [11]. The present method proposed here utilizes adsorption/desorption phenomena at the membrane surface to result in electric potential change.…”

Section: Resultsmentioning

confidence: 99%

Detection of Noncharged Organic Substances by New Measurement Method Using a Lipid Membrane Sensor

Naito

Ikezaki²,

Taniguchi³

et al. 2004

IEEE Sensors J.

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This paper reports a new measurement method to detect ppb levels of noncharged organic substances using lipid/ polymer membrane sensors. Noncharged organic substances have large influences on the adsorption of positively-charged lipids to negatively-charged membranes. Organic solvents (trichloroethylene) and endocrine disrupting chemicals (di-2-ethylhexylphthalate) were detected by utilizing the sensor output, which is the change of membrane potential caused by interactions among the lipid membrane, noncharged organic substances, and lipids in solution. This new potentiometric method has a possibility of detection of a trace amount of noncharged toxic substances.

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“…Nowadays potentiometric biosensors are of common practical usc. Their variants are described in a number of monographs [1, 2,3] and reviews [4,5,6]. Biosensors.…”

Section: Introductionmentioning

confidence: 99%

A Stable Urea Biosensor with Fluoride Ion-Selective Electrode 1. Theory

Katsakos

Birbilis

Neykov

1999

Biotechnology & Biotechnological Equipment

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The present paper deals with some theoretical problems of an inhibitor urea biosensor with immobilized urease and base ion-selective electrode for fluoride ions. The kinetics under homogeneous conditions is ana(vzed and the basic differential equations describing the behaviour of the inhibitor .~vstem are presented. A.flerwarcl.s the theoretical background and the behaviour of a biosensor with active a.~:vmmetric membrane under steat{y-state mode are anavzed. Ana(vtical expressions of the biosensor tran.~fer jimction for 5 dijjerent cases are obtained, including the case when no limitations of the inhibitor and substrate concentrations are necessm:v.

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Potentiometric Enzyme Methods

Cited by 12 publications

References 197 publications

Development and application of a histidine-selective biomembrane electrode

Development and application of a histidine-selective biomembrane electrode

Detection of Noncharged Organic Substances by New Measurement Method Using a Lipid Membrane Sensor

A Stable Urea Biosensor with Fluoride Ion-Selective Electrode 1. Theory

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