Background: Trichinellosis caused by the gastrointestinal nematode Trichinella
spiralis occurs in humans, domestic animals and wild animals. It is difficult
to control the muscle phase of the parasite. Homeopathic drugs such as Cina
and Santoninum have anthelmintic properties. We have observed that in material
doses, the homeopathic drug Podophyllum also has nematotoxic properties.
We have also observed that homeopathic potency can influence the
water permeability of cells. Objective: The purpose of this study was to investigate
whether potentized homeopathic drugs such as Cina 30, Santoninum
30 and Podophyllum mother tincture can affect the muscle phase of
the parasite T. spiralis in mice. Another objective was to see whether
trichinellosis and its treatment with the 3 named homeopathic drugs could
alter the water content in the muscle tissue of mice. Materials and Methods:
Cina 30 and Santoninum 30 were prepared from the mother tincture of the
flowering tops of Artemisia nilagirica and its active principle santonin, in each
case by successive dilution (1:100) with 90% ethanol and sonication in 30
steps following the single glass method (K30). Ethanol 30 was prepared by
successive dilution of 90% ethanol with 90% ethanol (1:100) followed by sonication
in 30 steps. In each step, the dilution was sonicated at 20 KHz for 30 s.
We have observed before that sonication is a more uniform, measurable and
effective process of mechanical agitation of a liquid than manual succussion.
Experimentally infected mice were orally treated with an aqueous Podophyllum
suspension at 60 mg/kg/day. Each potentized drug was diluted 1:20 with
distilled water and administered orally at 0.05 ml/mouse/day. Each mouse
was inoculated with T. spiralis larvae at a dose of 200 larvae/mouse by
esophageal intubation. Treatment was started on day 7 post-infection and
continued for 120 days. After completion of treatment, the mice were sacrificed
and the larvae were extracted from muscles by HCl-pepsin digestion.
The water content of the muscles was measured by determining the difference
between fresh weight and dry weight of the tissue. Results: Podophyllum
?, Cina 30 and Santoninum 30 reduced the larval population in the studied
mice by 68.14%, 84.10% and 81.20%, respectively, as compared to the untreated
control group. Ethanol 30 achieved no significant reduction in the larval
population compared to the untreated control group. The water content of
the muscle tissue in the untreated control group and the Podophyllum-treated
groups was significantly higher than in the Ethanol 30-, Cina 30- and Santoninum
30-treated groups. Conclusions: (1) Podophyllum ?, Cina 30 and
Santoninum 30 were effective in the muscle phase of T. spiralis infection and
significantly reduced the larval population in the treated mice. The potencies
were more effective than the mother tincture, an effect which was not due
to the medium ethanol. (2) The potencies significantly reduced the water
content of the muscle tissue which might have affected the larvae. The effect
of Po...