Poultry fat decreased fatty acid transporter protein mRNA expression and affected fatty acid composition in chickens

Abstract

BackgroundA study was undertaken to examine the effects of poultry fat (PF) compared with those of soybean oil (SBO) on intestinal development, fatty acid transporter protein (FATP) mRNA expression, and fatty acid composition in broiler chickens. A total of 144 day-old male commercial broilers were randomly allocated to 2 treatment groups (6 replicates of 12 chicks for each treatment) and fed isocaloric diets containing 3.0% PF or 2.7% SBO at 0 to 3 wk and 3.8% PF or 3.5% SBO at 4 to 6 wk, respectively.ResultsPF had no influence on intestinal morphology, weight, or DNA, RNA, or protein concentrations at 2, 4, and 6 wk of age. However, compared with SBO, PF significantly decreased FATP mRNA abundance at 4 wk (P = 0.009) and 6 wk of age (P < 0.001); decreased liver fatty acid-binding protein (L-FABP) mRNA abundance at 6 wk of age (P = 0.039); and decreased C18:2 (P = 0.015), C18:3 (P < 0.001), C20:2 (P = 0.018), Σ-polyunsaturated fatty acids (Σ-PUFA) (P = 0.020), and the proportion of PUFA (P < 0.001) in the intestinal mucosa and decreased C18:2 (P = 0.010), C18:3 (P < 0.001), C20:2 (P < 0.001), Σ-PUFA (P = 0.005), and the proportion of PUFA (P < 0.001) in breast muscle at 6 wk of age.ConclusionsPF decreases FATP and L-FABP mRNA expression and decreased the proportion of PUFA in the intestinal mucosa and breast muscle.

Tissue Distribution and Nutritional Regulation of Fatty Acid-Binding Proteins (fabps) in Two Marine Teleosts, Turbot (Scophthalmus maximus), and Tiger Puffer (Takifugu rubripes)

“…The response of fabp expression to dietary lipid level may also be related to the fatty acid profile of the diets. In general, different fabp isoforms reveal distinct binding preferences for specific fatty acids [35,49]. In this study, the higher lipid level was obtained by adding fish oil into the control diet, resulting in high LC-PUFA levels in high-lipid diets.…”

Heat stress modulates the disruptive effects of Eimeria maxima infection on the ileum nutrient digestibility, molecular transporters, and tissue morphology in meat-type chickens

“…Enterocytes also express an active sodium-glucose symporter (SGLT1) on the apical membrane that utilizes the concentration gradient of Na + to transfer glucose against its concentration [ 15 ]. Absorption of lipids and fatty acids occur through fatty acid-binding proteins (FABPs) and fatty acid transport proteins (FATPs) that are expressed on the apical membrane of the intestinal mucosal cells [ 16 , 17 ]. The di- and tripeptides are absorbed via H + -dependent peptide transporters (PEPT1 and PEPT2), and peptide/histidine transporter 1 (PHT1) [ 18 , 19 ].…”

ELOVL5 and VLDLR synergistically affect n-3 PUFA deposition in eggs of different chicken breeds

“…Moreover, direct feeding of ALA did not significantly affect FABP1 in the liver of laying hens ( Neijat et al, 2017 ). However, some studies have found that high levels of dietary fat can increase the expression of FATP and FABP mRNA in the liver ( Yuan et al, 2012 ), as well as the expression of FABP in the intestine ( Katongole and March, 1980 ). In our experiment, feeding flaxseed oil resulted in high expression of FABP1 and FATP1 in liver and duodenum.…”