“…Microcalorimetry has been used widely in life sci-ence, such as in the research of enzyme, 20,21 mitochondria, 22 bacteria, 23 animal cell, 13,24 etc, and is the direct and accurate method to study energy metabolism of living materials. Compared to the routine biological methods, microcalorimetry is very sensitive and can investigate the research system continuously without interfering with it.…”