Practical Antibody Recruiting by Metabolic Labeling with Caged Glycans

Abstract

We propose a de novo glycan display approach that combines metabolic labeling and a glycancaging strategy as a facile editing method for cell-surface glycans. This method enables the introduction of antigen glycans onto cancer cells to induce immune responses through antibody recruiting. The caging strategy prevents the capture of α-rhamnose (an antigen glycan) by endogenous antibodies during the introduction of the glycan to the targeted cell surface, and subsequent uncaging successfully induces immune responses. Therefore, this study proposes a practical method for editing the cell-surface glycocalyx under promiscuous conditions, such as those in vivo, which paves the way for the development of glycan function analysis and regulation.

In Situ Glycan Analysis and Editing in Living Systems

“…However, the lack of temporal or spatial selectivity in glycan editing is a major drawback for such strategies. Fukase’s group recently introduced temporal control by installing a photocleavable cage on rhamnose, which is a practical strategy for editing the cell-surface glycocalyx under promiscuous conditions …”

“…Fukase's group recently introduced temporal control by installing a photocleavable cage on rhamnose, which is a practical strategy for editing the cell-surface glycocalyx under promiscuous conditions. 183 The use of glycosyltransferases is also common for in situ glycan editing. For example, α-1,3-fucosyltransferase can convert the native CD44 glycoform on mesenchymal stromal cells to hematopoietic cell E-selectin/L-selectin ligand (HCELL), which results in osteoid generation in mice.…”

“…Fukase’s group recently introduced temporal control by installing a photocleavable cage on rhamnose, which is a practical strategy for editing the cell-surface glycocalyx under promiscuous conditions. 183 …”