Practical Aspects in the Production and Use in Elisa of Antisera Against Viruses in Bulbous Crops

Derks, A.F.L.M.; Lemmers, M.E.C.; Vlugt, C.I.M. van der

doi:10.17660/actahortic.1988.234.21

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Virus and Antiserum1

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1993

1994

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“…Packaged recombinant phage DNA was plated on Escherichia coli strain Y1090. The library was screened [37,45], using 20~ normal horse serum (for blocking) and 0.07 mg/ml purified antibodies of ISMV-antiserum (titer serum: 1/160) directed to purified virions [10] and one positive clone was isolated. DNA was isolated from the positive plaques and subcloned into plasmid pTZ-19R (Bio-Rad).…”

mentioning

confidence: 99%

Molecular cloning and sequence analysis of the 3′-terminal region of iris severe mosaic virus RNA

Vlugt¹,

Langeveld

Goldbach³

1994

Archives of Virology

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mentioning

confidence: 99%

Molecular cloning and sequence analysis of the 3′-terminal region of iris severe mosaic virus RNA

Vlugt¹,

Langeveld

Goldbach³

1994

Archives of Virology

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“…Antiserum. the gamma globulin fraction and conjugate were prepared as described by Derks. Lemmers & Van der Vlugt (1988).…”

Section: Virus and Antiserummentioning

confidence: 99%

Improved detection of iris severe mosaic virus in secondarily‐infected iris bulbs

Vlugt¹,

Derks²,

Boonekamp³

et al. 1993

Annals of Applied Biology

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Summary The influence of wounding and high‐temperature treatment on the detection of iris severe mosaic virus (ISMV) in secondarily ISMV‐infected iris bulbs was studied. Wounding of the bulbs just after lifting, followed by storage for 3 wk at 17°C or 20°C, increased the detectability of ISMV to 100% reliability. High‐temperature treatment and consecutive storage at 17°C induced a similar improvement of detection. It is concluded that a certain degree of stress, such as wounding or high‐temperature treatment, ultimately leads to an increase in viral antigens and thus to improvement of detection. It is hypothesised that the virus titre increases by the altered metabolism during the repair reactions as a response to stress applied to the bulbs.

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Practical Aspects in the Production and Use in Elisa of Antisera Against Viruses in Bulbous Crops

Cited by 2 publications

References 0 publications

Molecular cloning and sequence analysis of the 3′-terminal region of iris severe mosaic virus RNA

Molecular cloning and sequence analysis of the 3′-terminal region of iris severe mosaic virus RNA

Improved detection of iris severe mosaic virus in secondarily‐infected iris bulbs

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