Practical aspects of bioreactor application in mass propagation of plants

Takayama, Shinsaku; Akita, Motomu

doi:10.1007/1-4020-3200-5_4

Cited by 22 publications

(11 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ment of several low-cost bioreactors for plant organ and whole plant culture. Monocotyledonous plant species are particularly well suited to liquid culture (Takayama and Akita, 2005). Temporary immersion systems (TIS) have optimized gaseous and solute transfer properties resulting in more or larger plants with greater dry weight for many crop species (Berthouly and Etienne, 2005).…”

mentioning

confidence: 99%

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Adelberg¹,

Cousins²

2006

HortSci

View full text Add to dashboard Cite

Geophytes store carbohydrates in modified underground shoot systems protected by a broad array of biologically active chemistry. In vitro formation of storage organs requires months in the lab instead of years in the field, when water and nutrients are correctly supplied. Liquid and agar systems in large and small vessels were compared for sugar and water use with turmeric (Curcuma longa) as a model plant. Small jars on a shaker were compared with large, flat-bottomed vessels containing thin films of liquid media, intermittently tilted at slight inclines that allow the advantages of liquid phase transfer with gentle agitation. Liquid culture in small vessels on a shaker yielded the most plants and liquid culture on a thin-film rocker in a large vessel yielded the largest plants. Increased and improved biomass (fresh and dry) in liquid culture compared to agar was based on greater sugar use. When large vessels of liquid media were grown for 5 and 6 months on a rocker, 400 mL of media yielded 150 to 200 g (fresh weight) of plants. Similarly, 13 to 16 g (dry weight) of plant tissue was derived from 24 g of sugar. Plants were about one-third rhizome by fresh mass. Rhizomes had greater dry and fresh weight than leaves or roots, indicating solute actively accumulated in the rhizome. The rhizomes had normal morphology, characteristic pigments and fragrance, and rhizome extracts had strong antioxidant potential. The gentle rocking action of plantlets in sugar-containing liquid medium was demonstrated to produce functional storage organs.

show abstract

mentioning

confidence: 99%

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Adelberg¹,

Cousins²

2006

HortSci

View full text Add to dashboard Cite

show abstract

“…The use of simple Pyrex ® vessels (Hempfling & Preil, 2005;Takayama & Akita, 2005) and also plastic bags (Savangikar, Savangikar, Daga, & Pathak, 2005) was very effective. The use of simple Pyrex ® vessels (Hempfling & Preil, 2005;Takayama & Akita, 2005) and also plastic bags (Savangikar, Savangikar, Daga, & Pathak, 2005) was very effective.…”

Section: Process Engineering and Low-cost 'Bioreactor' Designmentioning

confidence: 99%

From Bioreactor to Entire Plants

Nguyen¹,

Dauwe²,

Bourgaud³

et al. 2013

New Light on Alkaloid Biosynthesis and Future Prospects

View full text Add to dashboard Cite

“…The induction of somatic embryogenesis has been recognized as a technique for producing large numbers of individuals and consequently, there is great interest in its use for mass propagation (von Arnold et al 2005;Preil 2005;Takayama and Akita 2005). However, before the implementation of this new methodology of propagation by somatic embryogenesis, the optimization and control of this process is required.…”

Section: Introductionmentioning

confidence: 98%

Influence of in vitro environment on somatic embryogenesis of Coffea arabica L. cv. Caturra rojo: the effects of carbon dioxide on embryogenic cell suspensions

Barbón

Jiménez

Preil

2008

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The influence of environment in the culture vessel is a factor that has very little study in the process of somatic embryogenesis. The present research was carried out with the objective to determine the effects of carbon dioxide on somatic embryogenesis of Coffea arabica cv. Caturra rojo. Embryogenic cell suspensions were cultured under different carbon dioxide concentrations (2.5%, 5.0%, and 10.0%) in the gases mixture and two control treatments, one with passive exchange and the other with forced ventilation. The results demonstrated that there were a larger number of somatic embryos formed with a concentration of 2.5% CO 2. The differentiation of these somatic embryos of coffee in embryogenic cell suspensions (130 9 10 3 SE l -1 ) was also stimulated. The effects of CO 2 on somatic embryogenesis were demonstrated when the control with passive exchange was compared with forced ventilation control, because in the former, where there was an accumulation of CO 2 , the production of somatic embryos was greater. CO 2 could stimulate the formation and differentiation of somatic embryos directly, which led to a modification of the pH patterns of the culture medium or indirectly when producing changes in the pH that favored the somatic embryogenesis process.

show abstract

Practical aspects of bioreactor application in mass propagation of plants

Cited by 22 publications

References 74 publications

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

From Bioreactor to Entire Plants

Influence of in vitro environment on somatic embryogenesis of Coffea arabica L. cv. Caturra rojo: the effects of carbon dioxide on embryogenic cell suspensions

Contact Info

Product

Resources

About