Practical strategy to construct anti-osteosarcoma bone substitutes by loading cisplatin into 3D-printed titanium alloy implants using a thermosensitive hydrogel

Jing, Zehao; Ni, Renhua; Wang, Jiedong; Lin, Xinhong; Fan, Daoyang; Wei, Qingguang; Zhang, Teng; Zheng, Yufeng; Cai, Hong; Liu, Zhongjun

doi:10.1016/j.bioactmat.2021.05.007

Cited by 45 publications

(29 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The porous structure was designed based on a bionic trabecular structure unit cell using CAD software (10). The porous Ti 6 Al 4 V scaffold was printed with a pore size of 640 μm, filament diameter of 400 μm, and porosity of 73%, as previously described (25).…”

Section: Sample Fabricationmentioning

confidence: 99%

Effect of micro-arc oxidation surface modification of 3D-printed porous titanium alloys on biological properties

Ni¹,

Jing²,

Xiong³

et al. 2022

Ann Transl Med

Self Cite

View full text Add to dashboard Cite

Background: Three-dimensional (3D) printing technology has been widely used in orthopedics; however, it is still limited to the change of macroscopic structures. In order to further improve the biological properties of 3D-printed porous titanium scaffolds, this study introduced micro-arc oxidation (MAO) technology to modify the surface of porous titanium scaffolds and construct bioactive coatings on the surface of porous titanium scaffolds to improve the biocompatibility and osseointegration ability of the material.Methods: For in vitro experiments, human bone marrow stem cells (hBMSCs) were seeded onto untreated scaffolds (control group) and MAO-treated scaffolds (experimental group). After 24 h of co-culture, cytotoxicity was observed using live/dead staining, and cell/scaffold constructs were retrieved and processed for the assessment of cell morphology by using scanning electron microscopy (SEM). Cell proliferation was detected using the Cell Counting Kit-8 (CCK-8) assay after 3, 7, and 14 days of co-culture. The levels of alkaline phosphatase (ALP) in the cell supernatant were detected after 7 and 14 days of co-culture. For in vivo experiments, micro-computed tomography (micro-CT) and Masson Goldner's staining were used to evaluate bone ingrowth and osseointegration at 4 and 8 weeks postoperatively.Results: In vitro experiment results confirmed that the two groups of scaffolds were non-cytotoxic and the cell adhesion status on the MAO-treated scaffolds was better. Over time, cell proliferation and ALP levels were higher in the MAO-treated group than in the untreated scaffolds. In the in vivo experiments, the MAOtreated scaffolds showed better bone ingrowth and osseointegration than the untreated group at different time points. Conclusions:The MAO-treated porous titanium scaffold formed a uniform and dense bioactive coating on the surface, which was more conducive to cell adhesion, proliferation, and differentiation and showed better osseointegration and bone ingrowth in vivo.

show abstract

Section: Sample Fabricationmentioning

confidence: 99%

Effect of micro-arc oxidation surface modification of 3D-printed porous titanium alloys on biological properties

Ni¹,

Jing²,

Xiong³

et al. 2022

Ann Transl Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the study of Jing et al, a PLGA-polyethylene glycol-PLGA temperature-sensitive hydrogel was used for the stable storage of cisplatin. The local temperature of the implant can be used as a “switch” for the release of cisplatin, as schematically represented in Figure 4 D. The results of experiments in a rat tumor model show that the implants of the experimental group had better anti-osteosarcoma effects and fewer side effects, and also had a positive effect on osteogenesis [ 76 ]. However, the simple hydrogel covering the surface of the titanium implants may have the problem of unstable bonding and easily slipping off after being exposed to external force.…”

Section: Antitumor Effectsmentioning

confidence: 99%

“…( C ) The experimental scheme of Zhang et al; reprinted with permission from [ 74 ]; copyright 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. ( D ) The experimental scheme of Jing et al; reprinted with permission from [ 76 ]; copyright 2021 Zehao Jing et al ( E ) Images of tumors collected from tumor-bearing mice after various treatments; reprinted with permission from [ 74 ]; copyright 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. ( F ) The experimental scheme of Sarkar et al; reprinted with permission from [ 77 ]; copyright 2020 American Chemical Society.…”

Section: Figurementioning

confidence: 99%

Titanium Implants and Local Drug Delivery Systems Become Mutual Promoters in Orthopedic Clinics

Gao

Zhao

et al. 2021

Nanomaterials

View full text Add to dashboard Cite

Titanium implants have always been regarded as one of the gold standard treatments for orthopedic applications, but they still face challenges such as pain, bacterial infections, insufficient osseointegration, immune rejection, and difficulty in personalizing treatment in the clinic. These challenges may lead to the patients having to undergo a painful second operation, along with increased economic burden, but the use of drugs is actively solving these problems. The use of systemic drug delivery systems through oral, intravenous, and intramuscular injection of various drugs with different pharmacological properties has effectively reduced the levels of inflammation, lowered the risk of endophytic bacterial infection, and regulated the progress of bone tumor cells, processing and regulating the balance of bone metabolism around the titanium implants. However, due to the limitations of systemic drug delivery systems—such as pharmacokinetics, and the characteristics of bone tissue in the event of different forms of trauma or disease—sometimes the expected effect cannot be achieved. Meanwhile, titanium implants loaded with drugs for local administration have gradually attracted the attention of many researchers. This article reviews the latest developments in local drug delivery systems in recent years, detailing how various types of drugs cooperate with titanium implants to enhance antibacterial, antitumor, and osseointegration effects. Additionally, we summarize the improved technology of titanium implants for drug loading and the control of drug release, along with molecular mechanisms of bone regeneration and vascularization. Finally, we lay out some future prospects in this field.

show abstract

“…A total of 24 articles [ [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] ] are included in this special issue. These articles can be categorized in terms of authors, biomaterials and targeted clinical areas, and article types.…”

mentioning

confidence: 99%

“…Several articles in this special issue are on regulatory science and discuss the new standards, tools, and approaches to evaluate the safety, efficacy, quality, and performance of medical products [ 10 , 13 , 17 , 19 ]. This special issue also covers a wide range of article types including research article [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , 11 , 12 , 15 , 16 , [18] , [19] , [20] , [23] , [24] , [25] , [26] ], reviews [ 14 , 17 , 21 , 22 , 27 ], perspectives [ 10 , 13 ] and opinion paper [ 18 ].…”

mentioning

confidence: 99%

Translation of biomaterials from bench to clinic

Zhang

Mikos

Reis

et al. 2022

Bioactive Materials

View full text Add to dashboard Cite

Practical strategy to construct anti-osteosarcoma bone substitutes by loading cisplatin into 3D-printed titanium alloy implants using a thermosensitive hydrogel

Cited by 45 publications

References 61 publications

Effect of micro-arc oxidation surface modification of 3D-printed porous titanium alloys on biological properties

Effect of micro-arc oxidation surface modification of 3D-printed porous titanium alloys on biological properties

Titanium Implants and Local Drug Delivery Systems Become Mutual Promoters in Orthopedic Clinics

Translation of biomaterials from bench to clinic

Contact Info

Product

Resources

About