Practice and Promise of Micropropagation of Woody Species

Anatomical responses to cytokinins of abscised grapevine shoot apices cultured in vitro

“…Upon release from apical dominance, these axillary meristems were capable of producing their own axillary meristems. The potential of cytokinins to stimulate development and elongation of axillary meristems, thus inducing in vitro shoot multiplication, is in agreement with reports for other woody species (De Fossard, 1976;Abbott, 1978;Cheng, 1978;Anderson, 1980;Hussey, 1980;Wang & Hu, 1980). Shoots derived from fragmented grapevine shoot apices were adventitious in origin (Barlass & Skene, 1980;Bar lass et al, 1981).…”

“…1978;Chee & Pool, 1982;Goussard, 1981Goussard, , 1982Harris & Stevenson, 1982). Most authors reporting on rapid in vitro propagation of woody plant species stressed the importance that proliferated shoots derived from detached apices should lead to the production of genetically uniform plants (De Fossard, 1976;Abbott, 1978;Cheng, 1978;Anderson. 1980).…”

“…1980). It is generally accepted (Murashige, 1974;Abbott, 1978;Hussey, 1980) that multicellular organized shoot meristems are genetically highly stable. Although adventitious meristems may enable a substantially faster cloning rate than axillary shoot multiplication, wholly mutant propagules may arise.…”

In vitro micropropagation of the tropical fruit tree Syzygium cuminii L.

“…Similarly, Hutchinson [5] observed that shoot tips, though commonly used, were not the best type of explant for initiating apple cultures because the majority produced relatively few shoots. Pistacia vera responded in a similar manner [3]. Shoot growth and proliferation was best on a medium containing 0.25/~M NAA ÷ 1.12 #M BA.…”

“…In vitro propagation of some woody plants has been achieved [1,2,4,8], but difficulties in establishing aseptic cultures from mature explants along with browning of explants have limited progress towards development of in vitro propagation of some species [3]. In the present study, aseptic seedlings were used as explant donors, and plantlets were obtained from uncontaminated material.…”