Clonal Propagation of Mature Trees: Problems and Possible Solutions

Bonga, J. M.

doi:10.1007/978-94-017-0994-1_15

Cited by 107 publications

(48 citation statements)

References 36 publications

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“…The media contained various concentrations (0.2-2.0 mg·l -1 ) of the cytokinin (6-benzylaminopurine (BAP) or (N-benzyl-9-(2-tetrahydropyranyl)adenine (BPA). For (Bonga, 1982(Bonga, , 1987Durzan, 1984Durzan, , 1990 (Schaffalitzky de Muckadell, 1954, 1959. Experiments with various tree species (Bonga, 1982(Bonga, , 1987Hartmann and Kester, 1983;Franclet et al, 1987) (Chalupa, 1979(Chalupa, , 1981(Chalupa, , 1983(Chalupa, , 1984Bellarosa, 1981;Pardos, 1981;Vieitez et al, 1985) and proved to be effective.…”

Section: Introductionmentioning

confidence: 99%

“…For (Bonga, 1982(Bonga, , 1987Durzan, 1984Durzan, , 1990 (Schaffalitzky de Muckadell, 1954, 1959. Experiments with various tree species (Bonga, 1982(Bonga, , 1987Hartmann and Kester, 1983;Franclet et al, 1987) (Chalupa, 1979(Chalupa, , 1981(Chalupa, , 1983(Chalupa, , 1984Bellarosa, 1981;Pardos, 1981;Vieitez et al, 1985) and proved to be effective. Recently the system has been refined (Bennett and Davies, 1986;Meier-Dinkel, 1987;Chalupa, 1988Chalupa, , 1990bSan-José et al, 1988, 1990 (Chalupa, 1987a(Chalupa, , 1990cSasaki et al, 1988;Gingas and Lineberger, 1989 …”

Section: Introductionmentioning

confidence: 99%

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Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

Chalupa

1993

Ann. For. Sci.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

Chalupa

1993

Ann. For. Sci.

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“…(Melchior, 1976). Eor trees, however, changes ohscr\'ed at frequent age inter\'als ha\'e never been measured or described mathematically, and this limits our ability to understand developmental cbanges (Bonga, 1987). If a set of measured cbaracteristics were available, it would tbeti be possible to construct tbe type of grapb envisaged by Borcbert (1976) to sbow curves wbicb describe tbe cbanges wbicb take place as a tree ages.…”

Section: Introductionmentioning

confidence: 99%

Developmental changes in Sitka spruce as indices of physiological age I. Changes in needle morphology

1989

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There is a paucity of data regarding developmental changes in trees. Measurements of length, width, length/width ratio, perimeter/width and width/height ratios of tranverse sections, projected and total surface areas, dry weight, specific leaf area and the weight of epicuticular waxes per unit leaf area were made on needles sampled from the uppermost whorl of Sitka spruce trees aged 3, 5, 10, 15, 20 and 38 years. All characteristics, except needle length and the weight of epicuticular waxes, showed asymptotic changes with age that could adequately be described by the Gompertz growth function, and offer potential as indices of physiological age. Asymptotes were reached at different ages (width and the length/width ratio having the slowest rates of change and the perimeter/width ratio, specific leaf area, and the width/height ratio having the fastest) suggesting a predictable sequence of changes. The observed changes in needle morphology are interpreted as a transition from shade to sun leaves.

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“…Os resultados obtidos no presente trabalho estão de acordo com as recomendações feitas por Bonga (1987), que a retirada de explantes deve ser feita preferencialmente partindo de brotações novas que são formadas durante a fase ativa de crescimento da planta, após o final da fase de dormência, durante a primavera e verão.…”

Section: Tabela 5: Influência Da Estação Do Ano Na Contaminação Fúngiunclassified

Estabelecimento de culturas assépticas de <i>Aspidosperma polyneuron</i>.

Ribas

Zanette²,

Kulchetscki³

et al. 2005

Ciênc. Florest.

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O presente trabalho objetivou estabelecer um protocolo para obtenção de culturas assépticas de Aspidosperma polyneuron visando à regeneração in vitro de mudas dessa espécie. Brotações apicais de mudas de dois anos de idade foram coletadas em casa de vegetação e desinfestadas com hipoclorito de sódio (NaOCl) ou cloreto de mercúrio (HgCl2) visando ao estabelecimento de culturas assépticas. Os tratamentos testados com NaOCl foram: solução 0,125 ou 0,25%, durante cinco ou dez minutos e com HgCl2: 0,025; 0,05 ou 0,1%, durante cinco ou dez minutos e em todas as estações do ano. As avaliações das percentagens de necrose, contaminação fúngica, contaminação bacteriana e de sobrevivência foram feitas após três semanas. Os resultados revelaram que o tratamento com solução de NaOCl a 0,25%, durante 10 minutos, resultou em 70% de sobrevivência para brotações apicais, independente da época do ano (verão e outono). O HgCl2 foi mais eficiente que o NaOCl na desinfestação de brotações apicais de peroba-rosa, sendo recomendado o tratamento de 0,05% de HgCl2, durante 10 minutos (84,10% de sobrevivência). Em todas as estações do ano, foram estabelecidas culturas assépticas, contudo, os melhores resultados foram obtidos na primavera e verão.

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Clonal Propagation of Mature Trees: Problems and Possible Solutions

Cited by 107 publications

References 36 publications

Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

Developmental changes in Sitka spruce as indices of physiological age I. Changes in needle morphology

Estabelecimento de culturas assépticas de <i>Aspidosperma polyneuron</i>.

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