Pre–B cell colony–enhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis

Jia, Song; Li, Yue; Parodo, Jean; Kapùs, András; Fan, Lingzhi; Rotstein, Ori D.; Marshall, John C.

doi:10.1172/jci19930

Cited by 529 publications

(246 citation statements)

References 65 publications

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“…Pbef1 is upregulated in neutrophils and monocytes by interleukin-1β, and it functions as a novel inhibitor of apoptosis in response to a variety of inflammatory stimuli during experimental inflammation and clinical sepsis [90]. Its expression is also induced in mammals by lipopolysaccharide and pro-inflammatory cytokines during the infection of human foetal membranes [91].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Changes in Spleens and Livers of Tumour-Bearing Mice Suggest Delayed Inflammation and Attenuated Cachexia in Response to Oil Palm Phenolics

et al. 2013

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Background/Aim: Plant phenolics can inhibit, retard or reverse carcinogenesis, and may thus help prevent or treat cancer. Oil palm phenolics (OPP) previously showed anti-tumour activities in vivo via a cytostatic mechanism at 1,500 ppm gallic acid equivalent. Here, we report other possible molecular mechanisms by which this extract attenuates cancer, especially those concerning the immune response. Methods: We subcutaneously injected J558 myeloma cells in BALB/c mice and supplemented OPP orally at 1,500 ppm gallic acid equivalent. We observed the physiology parameters of these animals and harvested their spleens and livers after 18 h, 1 week and 4 weeks for microarray gene expression analysis using Illumina MouseRef-8 BeadChips. Results: Time course microarray analysis on spleens after injecting J558 myeloma cells in mice revealed that the immune response of tumour-bearing mice supplemented with OPP was lower compared to controls, thus suggesting delayed inflammation in response to OPP. In livers, cholesterol biosynthesis genes were upregulated while inflammatory genes were downregulated through time, further suggesting attenuation of systemic inflammation and cachexia. These effects correlated with the delayed in vivo development of syngeneic tumours in mice given OPP. Conclusions: This study suggests the possible utilisation of OPP as an anti-tumour and anti-cachexia agent.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Changes in Spleens and Livers of Tumour-Bearing Mice Suggest Delayed Inflammation and Attenuated Cachexia in Response to Oil Palm Phenolics

et al. 2013

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“…In addition, several studies have reported increased visfatin levels in type 2 diabetes mellitus with or without any correlation with insulin resistance [9,10,11]. Still other studies have reported the effects of visfatin in other conditions, such as metabolic syndrome [12,13,25,26], cardiovascular disease [12,15,26,27,28] and inflammatory status [11,15,16,17,18]. …”

Section: Discussionmentioning

confidence: 99%

“…Several studies have also reported its relationship with type 2 diabetes mellitus [9,10,11] as well as its positive or nonsignificant relationship with insulin resistance [7,12,13,14,15]. Visfatin has also been suggested to play a role in proinflammatory and immunomodulating processes [16,17,18]. …”

Section: Introductionmentioning

confidence: 99%

Differential Significance of Plasma Visfatin Concentrations according to Adiposity in Children and Adolescents

Kim¹,

Park²,

Choi³

et al. 2013

Horm Res Paediatr

2,189

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Background/Aims: Visfatin is an adipocytokine predominantly expressed in visceral adipose tissue. We examined the relationship between plasma visfatin concentrations and anthropometric and metabolic parameters in children and adolescents, who are relatively less influenced by the effects of accompanying disease. Methods: We studied 135 children and adolescents (8-16 years old). Plasma glucose, insulin, lipid profile, visfatin and other adipocytokine levels were measured. CT scans were performed to evaluate the distribution of abdominal fat. We analyzed the relationship between circulating visfatin levels and anthropometric and metabolic parameters according to central adiposity (total abdominal fat by CT scan). Results: In the lower-adiposity group, plasma visfatin concentrations were significantly correlated with total abdominal fat, visceral fat, subcutaneous abdominal fat, plasma triglyceride level and homeostasis model assessment of insulin resistance (HOMA-IR). In a multiple linear regression analysis, visceral fat and triglycerides were independently associated with plasma visfatin levels. In the higher-adiposity group, plasma visfatin concentrations were not correlated with visceral fat or HOMA-IR but were significantly correlated with circulating interleukin-6 levels. Conclusion: These data suggest that the relationship between plasma visfatin concentrations and metabolic parameters differs according to central adiposity and that plasma visfatin concentrations are correlated with visceral fat and triglyceride levels, especially in children and adolescents with lower adiposity.

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“…Alternatively known as the pre-B cell colony-enhancing factor, studies suggest that visfatin acts as an insulin-mimetic, capable of binding to the insulin receptor and inducing insulin-like effects [1,2]. Associated with obesity and regulated by cytokines known to promote insulin resistance, these observations indicate that visfatin may have a role in the pathogenesis of type II diabetes [3].…”

Section: Introductionmentioning

confidence: 99%

Visfatin Reduces Gap Junction Mediated Cell-to-Cell Communication in Proximal Tubule-Derived Epithelial Cells

Hills

Kerr

Wall

et al. 2013

Cell Physiol Biochem

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Background/Aims: In the current study we examined if the adipocytokine, visfatin, alters connexin-mediated intercellular communication in proximal tubule-derived epithelial cells. Methods: The effects of visfatin (10-200ng/mL) on cell viability and cytotoxicity in HK2-cells were assessed by MTT, crystal violet and lactate dehydrogenase assays. Western blot analysis was used to confirm expression of Cx26, Cx40 and Cx43. The effect of visfatin (10-200ng/mL) on TGF-β1 secretion was confirmed by ELISA, and the effects of both TGF-β1 (2-10ng/mL) and visfatin (10-200ng/mL) on connexin expression were assessed by western blot. Functional intercellular communication was determined using transfer of Lucifer Yellow and paired-whole cell patch clamp electrophysiology. Results: In low glucose (5mM), visfatin (10-200ng/mL) did not affect membrane integrity, cytotoxicity or cell viability at 48hrs, but did evoke a concentration-dependent reduction in Cx26 and Cx43 expression. The expression of Cx40 was unaffected. At 48hrs, visfatin (10-200ng/mL) increased the secretion of TGF-β1 and the visfatin-evoked changes in connexin expression were mimicked by exogenous application of the pro-fibrotic cytokine (2-10ng/ml). Visfatin reduced dye transfer between coupled cells and decreased functional conductance, with levels falling by 63% as compared to control. Although input resistance was increased following visfatin treatment by 166%, the change was not significant as compared to control. The effects of visfatin on Cx-expression and cell-coupling were blocked in the presence of a TGF-β1 specific neutralizing antibody. Conclusions: The adipocytokine visfatin selectively evoked a non-toxic reduction in connexin expression in HK2-cells. The loss in gap-junction associated proteins was mirrored by a loss in functional conductance between coupled cells. Visfatin increased TGF-β secretion and the pattern of change for connexins expression was mimicked by exogenous application of TGF-β1. The effect of visfatin on Cx-expression and dye transfer were negated in the presence of a TGF-β1 neutralising antibody. These data suggest that visfatin reduces connexin-mediated intercellular communication in proximal tubule-derived epithelial cells via a TGF-β dependent pathway.

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Pre–B cell colony–enhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis

Cited by 529 publications

References 65 publications

Gene Expression Changes in Spleens and Livers of Tumour-Bearing Mice Suggest Delayed Inflammation and Attenuated Cachexia in Response to Oil Palm Phenolics

Gene Expression Changes in Spleens and Livers of Tumour-Bearing Mice Suggest Delayed Inflammation and Attenuated Cachexia in Response to Oil Palm Phenolics

Differential Significance of Plasma Visfatin Concentrations according to Adiposity in Children and Adolescents

Visfatin Reduces Gap Junction Mediated Cell-to-Cell Communication in Proximal Tubule-Derived Epithelial Cells

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