Pre-challenge Evaluation of Immune Response in Serum Cytokines (Th1 and Th2) and T-cell Markers (CD4, CD8, CD3, and CD25) Following Administration of New Formulated Leishmania Vaccine in Balb/c Mice

Evaluation of New Leishmania Major Vaccine Against Immune Responses (Serum’s IL-17 and IL-23 and Spleen White Pulp Changes) Post Challenging with Leishmania Amastigotes in Balb/c Mice

“…It can be argued that this adjuvant is better than BCG and causes the mice to exhibit stronger immune responses that are likely go toward Th1 immune response. Our results were satisfactory and confirm our previous results [7][8][9][10][11][12][13][14][15]. In this experiment all of mice in all groups were alive and killed before just the end of experimental step of study.…”

“…Then the collected parasites reached the concentration of 5.92 × 10 ¹˚, and were divided into five equal volume tubes. They were ready to prepare antigen for vaccine preparation like before [7][8][9][10][11][12][13][14][15].…”

“…Detailed procedures have already been described [7][8][9][10][11][12][13][14][15]. In short, just before the injection, the BCG vaccine "SSI" was suspended in a suspension of SST and 0.1 mg BCG (first dose) or 0.01 mg BCG (consecutive dose) was added to each bottle containing promastigote.…”

“…BCG adjuvant for each injection dose is included 2 × 10 CFU/0.1 ml. To prepare Teucrium polium adjuvant : 400 mg of Teucrium polium alcoholic extract in 1 ml distilled water without endotoxin deionized, 205 mg/0.1 ml was used for each of the doses of the leishmania antigens given above, and two injection doses containing 100 μg/0.1 ml antigens or 200 μg/0.1 ml containing adjuvants [7][8][9][10][11][12][13][14][15].…”

“…For detailed procedures please refer to Latifynia and collaborations [7][8][9][10][11][12][13][14][15]. In brief, Balb/c and traditional white laboratory mice (n=48) were obtained at three months old from the Pasteur Institute.…”