2017
DOI: 10.1016/j.vaccine.2016.11.080
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Pre-clinical development of a hydrogen peroxide-inactivated West Nile virus vaccine

Abstract: West Nile virus (WNV) is a mosquito-transmitted pathogen with a wide geographical range that can lead to long-term disability and death in some cases. Despite the public health risk posed by WNV, including an estimated 3 million infections in the United States alone, no vaccine is available for use in humans. Here, we present a scaled manufacturing approach for production of a hydrogen peroxide-inactivated whole virion WNV vaccine, termed HydroVax-001 WNV. Vaccination resulted in robust virus-specific neutrali… Show more

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Cited by 19 publications
(54 citation statements)
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“…The most severe lesions were seen in the hippocampus. In contemporary work, the use of rats is primarily related to toxicology studies which assess for the safety of WNV vaccines [188][189][190].…”
Section: Additional Animal Species Used In Wnv Biomedical Researchmentioning
confidence: 99%
“…The most severe lesions were seen in the hippocampus. In contemporary work, the use of rats is primarily related to toxicology studies which assess for the safety of WNV vaccines [188][189][190].…”
Section: Additional Animal Species Used In Wnv Biomedical Researchmentioning
confidence: 99%
“…The HydroVax-001 vaccine is a West Nile virus-Kunjin strain, Vero cell tissue culture-derived, purified whole virion vaccine inactivated with 3% hydrogen peroxide. HydroVax-001 drug product contains 4 mcg purified whole virion WNV formulated in a volume of 0.5 mL/dose with 0.1% aluminum hydroxide and 2% sorbitol in phosphate-buffered saline [27]. Sodium Chloride Injection USP 0.9% as a sterile, nonpyrogenic, isotonic solution was used as the vaccine diluent and the placebo.…”
Section: Methodsmentioning
confidence: 99%
“…As an alternative to traditional formaldehyde-based vaccines, a novel hydrogen peroxide (H 2 O 2 ) inactivation approach has been developed to produce a first-generation whole-virus vaccine against WNV that was tested at concentrations ranging from 1 to 40 mcg/dose [2527]. Preliminary studies explored the use of H 2 O 2 -based inactivation of wild-type NY99 WNV for vaccine development, with vaccine antigen able to elicit robust neutralizing antibody responses in amice and protect against lethal challenge [27]. Further studies using WNV-Kunjin virus (WNV-KV), a naturally attenuated Lineage 1 WNV strain, expanded on these preliminary results [26].…”
Section: Introductionmentioning
confidence: 99%
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“…Another relatively easy target is West Nile Virus. 53 This infection started in Africa, spread to North Africa, Europe and eventually the United States, where it migrated from New York City to virtually the entire country through mosquito-borne infection of birds, from which vector mosquitoes could transmit the virus to humans. 54 Although the incidence of West Nile Virus has decreased recently, presumably because the reservoir has diminished due to death of many infected birds and postinfection immunity in others, there were still over 2,000 cases in the US in 2015.…”
Section: The Short Term: Filoviruses and Chikungunyamentioning
confidence: 99%