Pre-Implantation Conceptus and Maternal Uterine Communications: Molecular Events Leading to Successful Implantation

Imakawa, Kazuhiko; Chang, Kyu‐Tae; Christenson, R. K.

doi:10.1262/jrd.50.155

Cited by 39 publications

(30 citation statements)

References 129 publications

(119 reference statements)

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“…Although numerous molecular studies have been conducted on the endometrium at the implantation stage, only a few have performed a morphological evaluation and have established a relationship between endometrial insufficiency and its morphology in RIF patients (15)(16)(17). Therefore, this study was designed to examine the morphological features of the endometrium at the implantation stage by TEM in RIF patients compared to fertile controls.…”

Section: Discussionmentioning

confidence: 99%

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Bahar¹,

Kahraman²,

Eras³

et al. 2015

Turk J Med Sci

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The aim of this study was to compare the cellular properties of endometrial tissues from fertile patients and patients having at least 3 previous in vitro fertilization failures, during the implantation window. The ultrastructural evaluation of the endometrium in the implantation window may shed light on the complexity of the implantation failure paradigm. Materials and methods:The study involved 23 women, 14 infertile with a clinical diagnosis of repeated implantation failure (RIF) and 9 fertile, defined as the control group. Endometrial samples were examined by transmission electron microscopy (TEM).Results: In the control group, secretory vacuoles and cytoplasmic projections filled with secretory material, called pinopodes, were noted; microvilli were observed on some apical surfaces; and ciliated cells were absent. In the RIF group, the number of pinopodes was remarkably lower, with some of them being immature. Moreover, decidualization of stromal cells was not frequent and fewer epithelial cells with poor secretory vacuoles were discerned. Conclusion:TEM analyses of endometrial samples from the RIF group revealed dramatic differences at the ultrastructural level compared to the controls, which may well be an underlying cause of their infertility.

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Section: Discussionmentioning

confidence: 99%

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Bahar¹,

Kahraman²,

Eras³

et al. 2015

Turk J Med Sci

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“…Thus, the lack of direct contact of the myometrium with embryonic secretes and weak myometrial production of estrogens during early pregnancy may result in local "downregulation" of OTR mRNA in the myometrium. Moreover, growth factors and cytokines secreted by embryos during the periimplantation period [59][60][61] indicate a possible influence on OTR gene expression in uterine tissues.…”

Section: Discussionmentioning

confidence: 99%

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Franczak

Bogacki

2009

J. Reprod. Dev.

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Abstract. In the pig, the periimplantation period is critical for successful establishment of pregnancy. We studied the influence of embryos on oxytocin (OT) and progesterone (P4) regulated endometrial and myometrial secretion of 1) luteotrophic prostaglandin E2 (PGE2) and 2) luteolytic prostaglandin F2α and its metabolite (PGFM) on days 12-14 of pregnancy in pigs. We used unilaterally pregnant pigs created by a surgical procedure in which one uterine horn remained intact and the second horn was cut transversely so that part of the horn was detached from the uterine body. The animals were divided into two groups, inseminated gilts (days 12-14 of pregnancy, n=6) and uninseminated cyclic gilts, which were used as controls (days 12-14 of estrous cycle, n=5). Embryos developed only in the patent part of the uterus and not in the occluded horn. The abundance of OTR mRNA was increased in the endometrium and decreased in the myometrium of the gravid uterine horn in the pregnant pigs compared with the non-gravid uterine horn or either uterine horn in the cyclic pigs, indicative of a local effect of the conceptus. The presence of embryos in the uterine horn during the periimplantation period determines endometrial metabolism of PGF2α and the local response of the endometrium to OT and P4. OT stimulates PGF2α secretion and PGFM accumulation in endometrial cultures only from the non-gravid uterine horn and controls PGE2 secretion from the endometrium and myometrium in the pregnant gilts. The results indicate a more systemic affect of pregnancy on the uterine response to OT and a possibly the local effect of the conceptus in promoting progesterone's inhibition of OT-stimulated PGE2 secretion and uterine metabolism of PGF2α. Key words: Embryos, Oxytocin, Pigs, Progesterone, Prostaglandin E2, Prostaglandin F2α and its metabolite, Uterus (J. Reprod. Dev. 55: [262][263][264][265][266][267][268][269][270][271][272] 2009) rostaglandins (PG) produced in the uterus and corpus luteum are necessary for successful reproduction [1,2]. Oxytocin, acting through oxytocin receptors (OTR) in the endometrium and myometrium, is involved in the control of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) secretion in pigs [3][4][5][6][7][8]. Prostaglandin F2α is responsible for luteal regression in non-pregnant pigs [1,9], while prostaglandin E2 acts as a luteotrophic and antiluteolytic factor that promotes maintenance of the corpus luteum during early pregnancy in pigs [10,11].Alteration of PG secretion by OT is implicated in maternal recognition of pregnancy in pigs [12][13][14]. Moreover, OT stimulates myometrial secretion of PGF2α and PGE2 as well as the contractile activity of the porcine myometrium during early pregnancy [8,15,16]. The uterine response to OT depends on expression of OTR mRNA and protein [7,[17][18][19] and on generation of the second messengers inositol triposhate and diacyloglycerol [7,18,20,21]. OT also stimulates PGF2α secretion from the bovine endometrium through a previously discussed signaling pathway [22][2...

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“…Prior to the initial development of the fertilised ovum to the blastocyst stage, the preparation of a receptive endometrium through the actions of progesterone (Rider 2002) and oestradiol ) is a prerequisite for the extraembryonic membranes-endometrial dialogue that supports conceptus attachment, implantation, development and survival (Imakawa et al 2004, Spencer et al 2004. In addition to the endometrial changes before implantation, other implantation and post-implantation changes in the endometrium may result from in utero presence of a conceptus.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Al-Gubory¹,

Arianmanesh²,

Garrel³

et al. 2014

Reproduction

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The expression and regulation of endometrial proteins are crucial for conceptus implantation and development. However, little is known about site-specific proteome profiles of the mammalian endometrium during the peri-implantation period. We utilised a two-dimensional gel electrophoresis/mass spectrometry-based proteomics approach to compare and identify differentially expressed proteins in sheep endometrium. Caruncular and intercaruncular endometrium were collected on days 12 (C12) and 16 (C16) of the oestrous cycle and at three stages of pregnancy corresponding to conceptus pre-attachment (P12), implantation (P16) and post-implantation (P20). Abundance and localisation changes in differentially expressed proteins were determined by western blot and immunohistochemistry. In caruncular endometrium, 45 protein spots (5% of total spots) altered between day 12 of pregnancy (P12) and P16 while 85 protein spots (10% of total spots) were differentially expressed between P16 and C16. In intercaruncular endometrium, 31 protein spots (2% of total spots) were different between P12 and P16 while 44 protein spots (4% of total spots) showed differential expression between C12 and C16. The pattern of protein changes between caruncle and intercaruncle sites was markedly different. Among the protein spots with implantation-related changes in volume, 11 proteins in the caruncular endometrium and six proteins in the intercaruncular endometrium, with different functions such as protein synthesis and degradation, antioxidant defence, cell structural integrity, adhesion and signal transduction, were identified. Our findings highlight the different but important roles of the caruncular and intercaruncular proteins during early pregnancy.

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Pre-Implantation Conceptus and Maternal Uterine Communications: Molecular Events Leading to Successful Implantation

Cited by 39 publications

References 129 publications

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Local and Systemic Effects of Embryos on Uterine Tissues During Early Pregnancy in Pigs

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

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