The purpose of this study is to analyse biotechnology topics for the STEM based-science practicum book. Preliminary study is need to be done before further research, especially for research and development or quasi experiment research. Biotechlogy is one popular topic from secondary school. Biotechnology constitutes a scientific study in science and technology which experiences rapid growth in recent years. The result shows that biotechology topics contain four basic elements to learn science such as fact, concept, principle and procedural. Biotechnology topic also had A1 until A5 level of affective skill and C1 until C4 level of knowledge skill and also P1 until P4 level of psychomotor skill. Every skill level on biotechlogy topic shows compability with the STEM framework. Biotechnology topics also led to a lot of integrated practicum activity especially for student in secondary school. Therefore, biotechnology topics is a compability topic for STEM Based-Science Practicum Book….