Pre-validation of a MALDI MS proteomics-based method for the reliable detection of blood and blood provenance

Kennedy, Katie; Heaton, Cameron; Langenburg, Glenn; Cole, Laura; Clark, Tom; Clench, Malcolm R.; Sears, Vaughn G.; Sealey, Mark; McColm, Richard; Francese, Simona

doi:10.1038/s41598-020-74253-z

Cited by 10 publications

(22 citation statements)

References 19 publications

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“…Kamanna, Henry, Voelcker, Linacre, and Kirkbride (2017) also used a proteomic approach concentrating on hemoglobins only to differentiate and map blood signatures from human and a range of Australian native mammals. Kennedy et al (2020) have subsequently validated the proteomic approach developed by Patel et al (2015) by MALDI MS on a total of 71 blind samples (stains and fingermarks) demonstrating that blood can be detected and discriminated from other biofluids and biofluid unrelated matrices. Semen markers were also detected in stains and in fingermarks, during this study.…”

Section: Bloodmentioning

confidence: 99%

The forensic exploitation of fingermark chemistry: A review

Bleay

Bailey

Croxton

et al. 2020

WIREs Forensic Science

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The substances deposited from the fingertip onto a surface during contact between them represent a highly complex range of chemicals that can be exploited in a variety of ways in a forensic investigation. An overview is given of the multitude of chemicals that have been detected in fingermarks, including those occurring in endogenous sweat, metabolites of ingested substances, and exogenous substances picked up on the fingertip. Changes in chemistry that may occur between deposition of the fingermark and its subsequent forensic analysis are discussed, with particular reference to the ways in which these changes have been considered as a means of dating fingermarks. The ways in which fingermark enhancement reagents utilize the different chemicals present to reveal ridge is reviewed, together with how different classes of chemical can be sequentially targeted to optimize the number of fingermarks recovered.A field of increasing interest is the use of advanced analytical techniques incorporating mass spectrometry and imaging capability to simultaneously obtain additional contextual information about the donor of the mark while visualizing the fingermark ridge pattern. Examples are given of how such information can be applied in forensic investigations. It is concluded that an extensive "tool kit" of fingermark enhancement processes is already available to utilize the different chemicals present, and the advances that can be made in this field using conventional approaches are limited. There is, instead, significant potential to utilize analytical techniques to forensically exploit the chemical information within fingermarks but there are also significant barriers to their implementation in this way.

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Section: Bloodmentioning

confidence: 99%

The forensic exploitation of fingermark chemistry: A review

Bleay

Bailey

Croxton

et al. 2020

WIREs Forensic Science

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“…Seminal fluid peptide biomarkers were also determined, which correspond to well-known seminal protein biomarkers, such as semenogelin I and II, prostate-specific antigen, and prostatic acid phosphatase. Kennedy et al devised a MALDI MS-based proteomic strategy that could reliably detect blood, discriminate human and animal blood, determine animal blood species, and identify semen (Figure 5) [44]. This approach can be used for the detection and provenance of blood with easy data acquisition, user-friendly data interpretation, and short analysis time.…”

Section: Identification Of Body Fluids and Tissuesmentioning

confidence: 99%

“…Identification (ID) levels I-III were for animal provenance (down to species) and ID levels IV-V were for determination of semen. Reprinted from [44], under the terms of the Creative Commons Attribution License (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ (accessed on 3 March 2021).…”

Section: Identification Of Body Fluids and Tissuesmentioning

confidence: 99%

Proteomics in Forensic Analysis: Applications for Human Samples

et al. 2021

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Proteomics, the large-scale study of all proteins of an organism or system, is a powerful tool for studying biological systems. It can provide a holistic view of the physiological and biochemical states of given samples through identification and quantification of large numbers of peptides and proteins. In forensic science, proteomics can be used as a confirmatory and orthogonal technique for well-built genomic analyses. Proteomics is highly valuable in cases where nucleic acids are absent or degraded, such as hair and bone samples. It can be used to identify body fluids, ethnic group, gender, individual, and estimate post-mortem interval using bone, muscle, and decomposition fluid samples. Compared to genomic analysis, proteomics can provide a better global picture of a sample. It has been used in forensic science for a wide range of sample types and applications. In this review, we briefly introduce proteomic methods, including sample preparation techniques, data acquisition using liquid chromatography-tandem mass spectrometry, and data analysis using database search, spectral library search, and de novo sequencing. We also summarize recent applications in the past decade of proteomics in forensic science with a special focus on human samples, including hair, bone, body fluids, fingernail, muscle, brain, and fingermark, and address the challenges, considerations, and future developments of forensic proteomics.

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“…[1][2][3][4][5][6] The compatibility with a range of blood enhancement techniques as well as its feasibility of application in aged blood (as old as 37 years 5 ) confer the technique operational significance. Recently, it has been possible to provide additional information from this evidence by establishing its human or animal provenance (down to animal species) as well as discriminating this from other biological matrices 4,[6][7][8][9] and in a pre-validation study. 6 Whilst reliable detection of blood and the additional information on blood provenance address the first lines of a forensic inquiry, other intelligence may also be useful and, in some cases, crucial in informing investigations.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, it has been possible to provide additional information from this evidence by establishing its human or animal provenance (down to animal species) as well as discriminating this from other biological matrices 4,[6][7][8][9] and in a pre-validation study. 6 Whilst reliable detection of blood and the additional information on blood provenance address the first lines of a forensic inquiry, other intelligence may also be useful and, in some cases, crucial in informing investigations. A prime example of vital intelligence is offered by DNA profiling, used to identify the perpetrator or to establish foul play in a murder inquiry without a body.…”

Section: Introductionmentioning

confidence: 99%