“…Prebiotics are substrates that are selectively used by the host microbiota, promoting the growth and metabolic activity of different microorganism groups, conferring a health benefit . Prebiotics are generally non-digestible oligosaccharides with a degree of polymerization between 4 and 30 that can be classified on the basis of their structure on fructooligosaccharides (FOS, fructose linked with a terminal glucose by β–2–1 bonds, with repetition of inulobiose, levanobiose, and sucrose), galactooligosaccharides (GOS, galactose linked by glycosidic bonds β–1–2, β–1–3, β–1–4, and β–1–6 with a terminal glucose), human milk oligosaccharides (HMO, composed of glucose, galactose, acetylglucosamine, fructose, or sialic acid with lactose at their reducing end), mannanoligosaccharide (MOS, obtained from partial hydrolysis of mannan polysaccharides; they can present an α–1–6 or β–1–4 bond), xylooligosaccharide (XOS, xylose residues linked through β–1–4 bonds), and arabinoxylooligosaccharide (AXOS, xylose backbone linked through β–1–4 bonds with arabinose substitution), to name a few, but other non-carbohydrate substrates are also recognized as prebiotics, such as conjugated linoleic acid (CLA), PUFAs, and polyphenols. − Byproducts are a great source of prebiotics (Table ), with different extraction techniques, such as thermal treatments, enzymatic extraction, and alkaline extraction, among others, and also throughout solid fermentation with fungi. The extraction technique and source have a great influence on the biological activity of these prebiotics.…”