1968
DOI: 10.1093/jaoac/51.5.1039
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Precise Fluor ometric Micro determination of Selenium in Agricultural Materials

Abstract: A precise, accurate, and comparatively rapid fluorometric method for the determination of selenium in the nanogram range is described and results are presented for Youden's uniformity and ruggedness tests. The sample is digested in a nitric-sulfuricperchloric acid mixture, potential interferences are masked with disodium EDTA, and selenium is complexed with freshly-prepared 2,3-diaminonaphthalene solution and estimated fluorometrically after extraction into cyclohexane. On the basis of its performance, collabo… Show more

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Cited by 45 publications
(14 citation statements)
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“…Selenium concentrations were determined by a fluorometric method using 2,3-diaminonaphtalene (Hoffman et al, 1968). Samples were digested with nitric acid and perchloric acid.…”
Section: Methodsmentioning
confidence: 99%
“…Selenium concentrations were determined by a fluorometric method using 2,3-diaminonaphtalene (Hoffman et al, 1968). Samples were digested with nitric acid and perchloric acid.…”
Section: Methodsmentioning
confidence: 99%
“…Selenium assay was conducted using the fluorometric procedure of Hoffman et al (1968). Diet ~-tocopherol was determined using the procedure of Sharp et al (1972b), and serum ~-tocopherol by the procedure of Fabianek et al (1968) as modified by Young et al (1976).…”
Section: Methodsmentioning
confidence: 99%
“…Indicators of iron status were whole blood haematocrit and serum ferritin. Plasma selenium levels were determined by a fluorometric method (Hoffman, Westerby & Hidiroglou, 1968) and the whole blood haematocrit by the microhaematocrit centrifugation. Serum ferritin levels were measured by a radioimmuno-assay using an Amersham kit (IM 1051, Amersham International plc, UK).…”
Section: Methodsmentioning
confidence: 99%