Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy

Abstract

Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30-70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas.

A feasibility study of the Nativis Voyager ® device in patients with recurrent glioblastoma in Australia

“…Voyager therapy is based on the ability of a time-varying magnetic field to exert force on point charge through a process known as magnetic induction, which is a fundamental principle of modern physics with application in biology and medicine [10]. The Voyager induces a biologic response in vitro and in vivo [11].…”

How far will the Voyager ® take us?

“…For people with cancer, Nativis developed the Voyager to deliver the recorded ulRFE cognate locally to the brain to induce a magnetic field that readily penetrates tissues. Preclinical studies suggest that some ulRFE cognates can regulate biological pathways and slow tumor development in xenograft models [12]. In that publication, it was shown the EGFR expression was reduced by a cognate to siRNA and that this led to decreased cell viability and survival of cultured cells and increased survival of mice with U87 tumors.…”

Evaluation of the EMulate Therapeutics Voyager’s ultra-low Radiofrequency Energy (ulRFE) in Murine Model of Glioblastoma (GBM)

“…At the heart of this technology are unique ulRFE signals, termed cognates. These cognates owe their genesis to a sophisticated, ultrasensitive magnetometer, designed to detect and record the subtle alterations in magnetic elds generated by solvated molecules (7)(8)(9)(10). Diving deeper into the spectrum of cognates, the ulRFE cognate A1A, inspired by the dynamics of paclitaxel, is hypothesized to hinder cell division and proliferation (10).…”