Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients

Assem, M; Kamal, Samia; Sabry, Dina; Soliman, Nadia A.; Aly, Riham Mohamed

doi:10.3889/oamjms.2018.076

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…MSC auto-transplantation protocols could be used to dramatically improve the outcomes of serious bony defects and non-unions. However, the limiting factor of any such treatment option is the time needed to expand an individual’s extracted stem-cell population [7]. Human bone marrow mesenchymal stem cells (hBM-MSCs) can be cultured using a variety of different techniques and methodologies, including traditional monolayer culture, biomatrix embedding of cells, and as of recently, self-assembled spheroid culture [8].…”

Section: Introductionmentioning

confidence: 99%

Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

Lawrence

Cottrill

Valluri

et al. 2019

IJMS

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Lack of standardization of clinically compliant culture protocols of mesenchymal stem cells for re-implantation in humans have hindered clinical progress in the field of tissue regeneration to repair maxillofacial and orthopedic defects. The goal of this study was to establish a clinically relevant osteogenic protocol for collection and expansion of autologous stem cells to be used at Marshall University for re-implantation and repair of maxillofacial and orthopedic conditions. Human bone marrow (hBM) samples were collected from patients undergoing intramedullary nail fixation for closed femoral fractures. hBM mesenchymal cells were expanded by growing them first in Petri dishes for two weeks, followed by a week of culture using Perfecta 3D Hanging Drop Plates®. Various scaffold materials were tested and analyzed for cellular integration, vitality, and differentiation capacity of harvested hBM-MSCs including: 60/40 blend of hydroxyapatite biomatrix; Acellular bone composite discs; Allowash®, cancellous bone cubes; PLGA (poly lactic-co-glycolic acid); and Woven chitin derived fiber. We found that the 3D spheroid culture allowed production of hBM mesenchymal cells that retained osteoblast differentiation capacity over a monolayer culture of hBM-MSCs without the need to use chemical or hormonal modulation. We also observed that hydroxyapatite and Allowash cancellous bone scaffolds allowed better cell integration and viability properties as compared to other materials tested in this study. In conclusion, the multimodal culture methodology we developed creates actively differentiating stem-cell spheroids that can then be readily utilized in clinical practices to improve the regeneration of tissues of the head and the body.

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Section: Introductionmentioning

confidence: 99%

Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

Lawrence

Cottrill

Valluri

et al. 2019

IJMS

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“…Moreover, PDL-MSCs had more proliferative potentials compared to G-MSCs in both healthy and diabetic conditions. All isolated cell populations showed positive expression of CD90 and CD105 and negative expression of CD45 without details of significant difference between diabetic and non diabetic cells [ 146 ]. Another study compared PDL-MSCs from teeth with periodontitis from diabetic patients to those extracted from non-diabetics both sound and with periodontitis.…”

Section: Tissue Engineering and Periodontal Regenerationmentioning

confidence: 99%

The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

et al. 2021

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Periodontitis and diabetes mellitus (DM) are two of the most common and challenging health problems worldwide and they affect each other mutually and adversely. Current periodontal therapies have unpredictable outcome in diabetic patients. Periodontal tissue engineering is a challenging but promising approach that aims at restoring periodontal tissues using one or all of the following: stem cells, signalling molecules and scaffolds. Mesenchymal stem cells (MSCs) and insulin-like growth factor (IGF) represent ideal examples of stem cells and signalling molecules. This review outlines the most recent updates in characterizing MSCs isolated from diabetics to fully understand why diabetics are more prone to periodontitis that theoretically reflect the impaired regenerative capabilities of their native stem cells. This characterisation is of utmost importance to enhance autologous stem cells based tissue regeneration in diabetic patients using both MSCs and members of IGF axis.

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“…The physical condition of donors is a key factor to properties of GMSCs. Assem et al revealed that GMSCs exhibited a greater proliferation rate and higher surviving in normal individuals than the diabetic patients [67]. Moreover, GMSCs exosome from diabetic mice showed reduced IL-1RA and decreased Fas expression when compared to WT GMSCs [40].…”

Section: Analysis Of Factors Influencing the Function Of Gmscmentioning

confidence: 99%

GMSC: Updates of Advances on Its Therapy in Immunological Diseases

Hou¹,

Zheng²

2020

Update on Mesenchymal and Induced Pluripotent Stem Cells

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Mesenchymal stem cells (MSCs) derived from various tissues are multi-potency of self-renewal and differentiation into multi-lineages, including chondrocytes, adipocytes and osteoblasts in vitro and in vivo. In addition, these cells also display potent immune regulatory roles that benefit the treatment of inflammatory and autoimmune diseases. We and others have previously identified that human gingival-derived mesenchymal stem cells (GMSCs) not only share similar biological features, but also display some potential advantages compared to other MSC populations. In the chapter, we have discussed the discovery, phenotypic and functional characteristics, as well as updated the advances of these cell therapies in immunological diseases.

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Preclinical Assessment of the Proliferation Capacity of Gingival and Periodontal Ligament Stem Cells from Diabetic Patients

Cited by 10 publications

References 32 publications

Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

Minimally Manipulative Method for the Expansion of Human Bone Marrow Mesenchymal Stem Cells to Treat Osseous Defects

The Effect of Diabetes Mellitus on IGF Axis and Stem Cell Mediated Regeneration of the Periodontium

GMSC: Updates of Advances on Its Therapy in Immunological Diseases

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