Preclinical study of experimental burns treated with photobiomodulation and Human Amniotic Membrane, both isolated and associated

Abstract

Objective: to evaluate the effect of photobiomodulation with low-level 660 nm laser alone or associated with Human Amniotic Membrane in the repair of partial-thickness burns in rats. Method: an experimental study conducted with 48 male Wistar rats, randomized into four groups: Control, Human Amniotic Membrane, Low-Level Laser Therapy, and Low-Level Laser Therapy associated with Human Amniotic Membrane. The histopathological characteristics of the skin samples were analyzed 7 and 14 days after the burn. The data obtained were submitted to the Kolmogorov-Smirnov and Mann-Whitney tests. Results: the histological analysis of the burn injuries showed a decrease in inflammation (p<0.0001) and an increase in proliferation of fibroblasts (p<0.0001) mainly at 7 days in all treatments related to the control group. At 14 days, the greater effectiveness in accelerating the healing process was significant (p<0.0001) in the Low-Level Laser Therapy group associated with the Human Amniotic Membrane. Conclusion: the association of photobiomodulation therapies with the Human Amniotic Membrane allowed verifying a reduction in the healing process time of the experimental lesions, stimulating its proposal as a treatment protocol in partial-thickness burns.

Fotomodulação no processo de cicatrização de queimaduras: Fotomodulação no processo de cicatrização de queimaduras: uma revisão integrativa

“…Considerou-se que os estudos incluídos, respondem como tem sido abordado na literatura científica, nacional e internacional, a utilização na fotobiomodulação no tratamento de queimaduras. Dentre eles, quatro são estudos experimentais (AMORIM et al;2023;GOMES et al,2017;KHAN et al, 2021;RANJBAR;TAKHFOOLADI, 2016) e três ensaios clínicos randomizados (KAZEMIKHOO et al, 2018;FREITAS et al, 2013;FREITAS et al, 2014). Os estudos foram publicados entre 2013 e 2023.…”

Low-Level Laser Therapy Promotes Wound Healing in Fibroblasts Isolated from Human Chronic Wounds

“…Recent studies have focused on exploring the potential of LED and laser technologies to enhance fibroblast activity and collagen synthesis, aiming to develop a cost-effective and practical approach for wound repair that does not rely on expensive and stationary light systems currently used in therapy. Since fibroblasts play a critical role in wound healing, many published studies on LLLT have investigated its impact on fibroblast cell growth, migration, and collagen production [6][7][8]. Experimental findings have shown that near-infrared (NIR) LED light treatment enhances mitochondrial oxidative metabolism in vitro and accelerates cell and tissue repair in vivo [9][10][11][12][13].…”