The world's expanding human population and rising standard of living result in a rise in energy consumption. Since fossil fuel reserves are mostly exploited to fulfil the expanding energy demand, this makes it more difficult to get energy. Due to the non-renewable nature of fossil fuel resources and the possibility of energy supply issues occurring as a result of globalization, access to energy becomes challenging at this point. However, switching to alternative and environmentally beneficial fuel sources is now a need because of the harm that their widespread usage has caused to the environment and the diminishing resources that are being used up. The loofah seed Luffa Aegyptiaca, which is grown mostly in Turkey's Hatay region, is discussed in this paper. Fatty acids in the oil produced from Luffa aegyptiaca seeds were identified by analysis. Oleic and linoleic acids were found to be the primary fatty acids controlling the combustion characteristics of biodiesel fuel. These acids were found to be, respectively, 97.8318 and 129.1163. Using the Biodiesel Analyzer v1.2, the physicochemical characteristics of biodiesel fuel were identified. It was determined that biodiesel fuel made from Luffa aegyptiaca seed might be used as a substitute for diesel fuel.