Predicting overall viability of cord blood harvests

Pope, Belinda; Mitsakos, Katerina; Bilgin, Ayşe; Hokin, Bevan; Grant, Ross

doi:10.1111/j.1537-2995.2011.03386.x

Cited by 11 publications

(19 citation statements)

References 20 publications

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“…Several studies have shown that the functionality of CD34+ cells can be reduced significantly after cryopreservation . In one of these studies, annexin V–negative CD34+ cells correlated with NOD/SCID mouse hematopoietic repopulating ability, indicating as would be expected that apoptotic CD34+ cells fail to engraft . Our results have extended these studies by showing a significant reduction in UCB CD34+ cell viability with both the 7‐AAD and annexin V methods used after cryopreservation that is associated with time to cryopreservation.…”

Section: Discussionsupporting

confidence: 75%

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Storage time affects umbilical cord blood viability

et al. 2013

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 98%

Storage time affects umbilical cord blood viability

et al. 2013

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“…However, in this study, we found small but significant losses of CFUs, CD34+ cells, and TNCC that occur in CB processed at least 24 hours after delivery compared to those processed less than 10 hours from collection. Our findings are similar to those of Pope and colleagues who recently correlated decreasing viability with increased time from collection to freeze. These findings indicate that delays in processing could impact the yield of bankable CBUs.…”

Section: Discussionmentioning

confidence: 99%

Optimizing donor selection for public cord blood banking: influence of maternal, infant, and collection characteristics on cord blood unit quality

et al. 2013

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Background Banked unrelated donor umbilical cord blood (CB) has improved access to hematopoietic stem cell transplantation for patients without a suitably matched donor. In a resource-limited environment, ensuring that the public inventory is enriched with high-quality cord blood units (CBUs) addressing the needs of a diverse group of patients is a priority. Identification of donor characteristics correlating with higher CBU quality could guide operational strategies to increase the yield of banked high-quality CBUs. Methods Characteristics of 5267 CBUs donated to the Carolinas Cord Blood Bank, a public bank participating in the National Cord Blood Inventory, were retrospectively analyzed. Eligible CBUs, collected by trained personnel, were processed using standard procedures. Routine quality and potency metrics [post-processing total nucleated cell count (post-TNCC), CD34+, colony-forming units (CFUs)] were correlated with maternal, infant, and collection characteristics. Results High-quality CBUs were defined as those with higher post-TNCC (>1.25×109), and CD34+ + CFU in the upper quartile. Factors associated with higher CD34+ or CFU content included a shorter interval from collection to processing (<10 hours), younger gestational age (34–37 weeks; CD34++CFU) Caucasian race, higher birth weight (>3500grams) and larger collection volumes (>80ml). Conclusions We describe characteristics identifying high-quality CBUs, which can be used to inform strategies for CBU collection for public banks. Efforts should be made to prioritize collections from larger babies born before 38 weeks of gestation. CBUs should be rapidly transported to the processing laboratory. The lower quality of CBUs from non-Caucasian donors highlights the challenges of building a racially diverse public CB inventory.

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“…Our conclusion is contradictory to the results of some authors who showed that the broad range of anticoagulant concentration did not affect cell viability, though a gradual loss of cell viability was observed after storage for 25-48 h and particular after 49-80 h [13]. But there are authors that suggest that the ratio of anticoagulant could determine low cell viability for the units with the collected UCB volume of less than 60 ml, even if the amount of anticoagulant in collection bags was lower (29 ml) [29].…”

Section: Tablementioning

confidence: 85%

“…To date, we identified only one study that indicated the collected UCB volume as a significant factor affecting the pre freeze cell viability and showed the combined effects of collected blood volume and time from collection to freezing on cell viability [29].…”

Section: Tablementioning

confidence: 99%