Prediction of anti-tumour effect of thermochemotherapy with<b><i>in vitro</i></b>thermochemosensitivity testing for non-small cell lung cancer

Abiko, Tomohiro; Kawamura, M.; Izumi, Yuichi; Oyama, Takahiko; Saito, Yoshimasa; Kobayashi, K

doi:10.1080/02656730701286333

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…The T and C values were calculated as an average of triplicate droplets. Primary tumor cells were collected using Collagen Gel Culture kit Primaster (Nitta Gelatin Inc) [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

Aquaporin 1 promotes sensitivity of anthracycline chemotherapy in breast cancer by inhibiting β-catenin degradation to enhance TopoIIα activity

et al. 2020

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Anthracyclines are a class of conventional and commonly used frontline chemotherapy drugs to treat breast cancer. However, the anthracycline-based regimens can only reduce breast cancer mortality by 20–30%. Furthermore, there is no appropriate biomarker for predicting responses to this kind of chemotherapy currently. Here we report our findings that may fill this gap by showing the AQP1 (Aquaporin1) protein as a potential response predictor in the anthracycline chemotherapy. We showed that breast cancer patients with a high level of AQP1 expression who underwent the anthracycline treatment had a better clinical outcome relative to those with a low level of AQP1 expression. In the exploration of the underlying mechanisms, we found that the AQP1 and glycogen synthase kinase-3β (GSK3β) competitively interacted with the 12 armadillo repeats of β-catenin, followed by the inhibition of the β-catenin degradation that led to β-catenin’s accumulation in the cytoplasm and nuclear translocation. The nuclear β-catenin interacted with TopoIIα and enhanced TopoIIα’s activity, which resulted in a high sensitivity of breast cancer cells to anthracyclines. We also found, the miR-320a-3p can attenuate the anthracycline’s chemosensitivity by inhibiting the AQP1 expression. Taken together, our findings suggest the efficacy of AQP1 as a response predictor in the anthracycline chemotherapy. The application of our study includes, but is not limited to, facilitating screening of the most appropriate breast cancer patients (who have a high AQP1 expression) for better anthracycline chemotherapy and improved prognosis purposes.

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“…The T and C values were calculated as an average of triplicate droplets. Primary tumor cells were collected using Collagen Gel Culture kit Primaster (Nitta Gelatin Inc) [ 10 , 11 ].…”

Section: Methodsmentioning

confidence: 99%

Aquaporin 1 promotes sensitivity of anthracycline chemotherapy in breast cancer by inhibiting β-catenin degradation to enhance TopoIIα activity

et al. 2020

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“…In addition, thermotherapy may enhance the cytotoxic effect of irradiation on S-phase cells and alleviate the toxicity reaction of normal tissue in selected cases (11). As a result, through increasing the blood supply of the surrounding normal tissue, thermotherapy enhances the biological effect of radiation (12).…”

Section: Discussionmentioning

confidence: 99%

Optimized treatment with RF thermotherapy and immunotherapy combined with CyberKnife for advanced high-risk tumors: A clinical trial report

et al. 2014

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This study was conducted to evaluate the application value of optimized treatment with radiofrequency (RF) thermotherapy and immunotherapy combined with CyberKnife for advanced high-risk tumors. The database of 1,013 patients with 2,136 tumor lesions and 1,237 target areas who underwent treatment with CyberKnife between November, 2010 and November, 2012, was retrospectively reviewed. We randomly assigned 505 eligible patients (observation group) to RF thermotherapy and adoptive immunotherapy with cytokine-induced killer cells and the remaining 508 patients (control group) to no adjuvant treatment. The patients in the two groups were recorded on efficacy assessment according to imageological examination, World Health Organization criteria, Karnofsky performance status, or radioimmunoassay (RIA) detection. The effective rate of the observation group was 75.05%, whereas that of the control group was 58.06% (P<0.05). The results revealed that CyberKnife combined with hyperthermia and biological therapy are highly effective in improving the local tumor control rate. Further analysis of the Karnofsky score and RIA detection confirmed that this type of combination therapy significantly improved the quality of life. The optimized treatment of RF thermotherapy and immunotherapy combined with CyberKnife may act synergistically in eliminating tumor cells, confirming the efficacy of this type of treatment for patients with advanced malignant tumors.

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“…Hyperthermia has emerged as a promising optional therapeutic modality for cancer treatment, and it functions by heating the regional tumor up to 42～43°C with a pair of electromagnetic plates. Although some antitumor effect is seen for thermotherapy used as monotherapy, it is augmented in combination with anticancer agents in clinical practice as reflected by the prolonged disease‐free survival (Abiko et al, 2007). Therefore, interest in combining chemotherapy with hyperthermia, also termed thermochemotherapy, is growing.…”

Section: Discussionmentioning

confidence: 99%

“…Although some antitumor effect is seen for thermotherapy used as monotherapy, it is augmented in combination with anticancer agents in clinical practice as reflected by the prolonged disease-free survival (Abiko et al, 2007). Therefore, interest in combining chemotherapy with hyperthermia, also termed thermochemotherapy, is growing.…”

Section: Thermochemotherapy Suppresses Sp1 and Its Downstream Target ...mentioning

confidence: 99%

ROS‐Sp1 axis is involved in thermochemotherapy‐enhanced sensitivity of pancreatic cancer cells to gemcitabine

Xi,

Ma,

et al. 2023

Cell Biology International

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Gemcitabine (GEM)‐based chemotherapy represents the first choice for locally unresectable advanced pancreatic cancer, while the benefit is limited due to acquired chemoresistance or drug delivery insufficiency. Hyperthermia treatment potentially improves the clinical efficacy of GEM. However, the underlying mechanism is incompletely revealed. Our study aims to investigate the effect and involved mechanism of thermochemotherapy on cell survival. Pancreatic cancer cells PANC‐1 and ASPC‐1 were either treated with GEM or thermochemotherapy, then cell viability, apoptosis, migration, invasion, reactive oxygen species (ROS) production, and Sp1 expression were evaluated. The results showed that GEM dose and time‐dependently affected cell viability, and 30 μM GEM achieved favorable effect in suppressing cancer cell growth. Meanwhile, hyperthermia preconditioning (43°C for 60 min) 24 h before GEM treatment yielded superior effect than other treatment sequence. GEM caused significant cell apoptosis and proapoptotic genes of both cancer cells, and thermochemotherapy further promoted apoptosis and genes transcription, accompanied by excessive ROS production. Thermochemotherapy was superior to GEM in suppressing cell migration and invasion of pancreatic cancer cells. Meanwhile, GEM significantly reduced Sp1 mRNA and protein and its downstream gene Cox‐2, and thermochemotherapy further suppressed their expressions. ROS elimination with N‐acetyl‐l‐cysteine notably neutralizes the suppressive effect of GEM and thermochemotherapy on cell growth and expressions of Sp1 and Cox‐2. In conclusion, thermochemotherapy inhibited pancreatic cell viability, migration and invasion, and promoted cell apoptosis by inducing excessive ROS production, which subsequently suppressed Sp1 expression and the downstream Cox‐2.

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Prediction of anti-tumour effect of thermochemotherapy within vitrothermochemosensitivity testing for non-small cell lung cancer

Abstract: A modified CD-DST using an exposure temperature of 42 degrees C can be used to predict the antitumour effect of thermochemotherapy.

Cited by 7 publications

References 22 publications

Aquaporin 1 promotes sensitivity of anthracycline chemotherapy in breast cancer by inhibiting β-catenin degradation to enhance TopoIIα activity

Aquaporin 1 promotes sensitivity of anthracycline chemotherapy in breast cancer by inhibiting β-catenin degradation to enhance TopoIIα activity

Optimized treatment with RF thermotherapy and immunotherapy combined with CyberKnife for advanced high-risk tumors: A clinical trial report

ROS‐Sp1 axis is involved in thermochemotherapy‐enhanced sensitivity of pancreatic cancer cells to gemcitabine

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