Prediction of in vivo bone forming potency of bone marrow-derived human mesenchymal stem cells

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

“…[1][2][3][4][5][6][7][8][9] Insights into the conductive and inductive biomolecules that control EC ossification have been essential to tissue engineering strategies focusing on cartilage formation, 57,58 osteochondral defects, 59,60 and, more recently, bone regeneration (Tables 2-4). 27,43,57,58,[61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76] While cartilage tissue engineering strategies have focused on inducing and maintaining chondrogenic phenotypes, the induction and modulation of cartilage hypertrophy is critical to the progression of EC ossification in bone regeneration designs. 9,33,34,[37][38][39][40][41][42][43]72,[77][78][79]…”

The Effect of Activating Fibroblast Growth Factor Receptor 3 Mutations on Osteogenic Differentiation and Ectopic Bone Formation by Human Periosteal Derived Cells

“…In the case of N540K, increased levels of these in vitro measures correspond to the elevated in vivo bone forming capacity. Proliferative ability of the cells has previously been determined to strongly correlate to in vivo bone forming capacity of bone marrow stromal cells [29]. Additionally, Coll1 expression was previously correlated to the bone forming capacity of PDCs [12,30].…”

The use of cellular technologies in the treatment of children with congenital cleft palate

“…3,4). Ткань механически измельчали, затем инкубировали при 37 °C в 0,2 % растворе колла-геназ (тип I, IV, Sigma-Aldrich, США) в ФСБ.…”

“…Для индукции остеогенной дифференцировки МСК в культуральную среду добавляют дексаметазон, аскорбиновую кислоту и β-глицерофосфат. Диф-ференцировка МСК в остеобласты подтверж-дается появлением мРНК остеокальцина, cdfa1, повышением активности щелочной фосфатазы, наличием внеклеточных преципитатов солей кальция [2][3][4][5].…”