To improve the prediction of nitrogen (N) uptake by sugar beet for the effective N fertilization, field experiments were carried out in 55 arable fields under various N management regimes throughout Hokkaido, Japan, from 2003 to 2006. The fields comprised nine soil types. Firstly, N uptake by sugar beet at different soil depths (0-20, 20-40, 40-60, 60-80, 80-100 cm) was examined using 15 N-labeled fertilizer in Andosol fields in 2005. Secondary, soil nitrate N at a depth of 0 to 60 cm in the fields was determined to analyze the relationship between soil nitrate N and N uptake by sugar beet. Thirdly, the effects of soil type, the quantity of organic materials and fertilizers applied, and the history of land use on N uptake by sugar beet were investigated. Although sugar beet took up nitrate N mainly at the soil depth of 0 to 60 cm, N uptake at the depth below 60 cm was not negligible. Moreover, soil nitrate content at the depth of 0 to 60 cm could not evaluate N released by decomposition of applied organic matter during the cropping season. Therefore, the evaluation of soil nitrate content at the depth of 0 to 60 cm was not practical for predicting N uptake by sugar beet. However, sugar beet N uptake could be predicted by the sum of N-score and applied fertilizer N amount using a linear regression analysis (N-score is the estimated value of N available to the next crop from the applied organic matter, the incorporated crop residue and the other field practices). This predictability may indicate that sugar beet effectively takes up N released from the applied N sources, including fertilizer and organic matters, partly because of the plant's deep root elongation, long cropping period and harvest in autumn when the decomposition of organic matter has passed its peak. As a result of this study, it was concluded that this methodology (N-score) could be useful for farmers to decide the most effective N fertilizer management for sugar beet.