Prediction of possible sites for posttranslational modifications in human gamma crystallins: Effect of glycation on the structure of human gamma‐b‐crystallin as analyzed by molecular modeling

Salim, Asmat; Bano, Asifa; Zaidi, Zafar H.

doi:10.1002/prot.10493

Cited by 10 publications

(3 citation statements)

References 49 publications

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“…Transmembrane domain prediction was done using TMHMM (v2.0) [38]. Finally, the prediction of putative N-glycosylation sites was done using NetNGlyc (v1.0) [39]. The gene organisation of the SaCD79α gene was determined by aligning the genomic sequence and cDNA sequence using the online Spidey program (http://www.ncbi.nlm.nih.gov/IEB/Research/Ostell/Spidey/) at NCBI.…”

Section: Methodsmentioning

confidence: 99%

B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)

Wang

Bird

et al. 2013

Fish & Shellfish Immunology

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CD79α (also known as Igα) is a component of the B cell antigen receptor complex and plays an important role in B cell signalling. The CD79α protein is present on the surface of B cells throughout their life cycle, and is absent on all other healthy cells, making it a highly reliable marker for B cells in mammals. In this study the spiny dogfish (Squalus acanthias) CD79α (SaCD79α) is described and its expression studied under constitutive and stimulated conditions. The spiny dogfish CD79α cDNA contains an open reading frame of 618 bp, encoding a protein of 205 amino acids. Comparison of the SaCD79α gene with that of other species shows that the gross structure (number of exons, exon/intron boundaries, etc.) is highly conserved across phylogeny. Additionally, analysis of the 5′ flanking region shows SaCD79α lacks a TATA box and possesses binding sites for multiple transcription factors implicated in its B cell-specific gene transcription in other species. Spiny dogfish CD79α is most highly expressed in immune tissues, such as spleen, epigonal and Leydig organ, and its transcript level significantly correlates with those of spiny dogfish immunoglobulin heavy chains. Additionally, CD79α transcription is up-regulated, to a small but significant degree, in peripheral blood cells following stimulation with pokeweed mitogen. These results strongly indicate that, as in mammals, spiny dogfish CD79α is expressed by shark B cells where it associates with surface-bound immunoglobulin to form a fully functional BCR, and thus may serve as a pan-B cell marker in future shark immunological studies.

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Section: Methodsmentioning

confidence: 99%

B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)

Wang

Bird

et al. 2013

Fish & Shellfish Immunology

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“…were predicted using NetNGlyc 1.0 Server [30] and the furin cleavage site predicted using ProP v1.0 Server [31]. Multiple sequence alignments (MSA) were generated using ClustalΩ (http://www.ebi.ac.uk/Tools/msa/clustalo/) [32].…”

Section: Sequence Analysis Of Sccd40l and Scbaffmentioning

confidence: 99%

Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark ( Scyliorhinus canicula )

Redmond

Wang

et al. 2015

Fish & Shellfish Immunology

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The tumour necrosis factor superfamily (TNFSF) members CD40L and BAFF play critical roles in mammalian B cell survival, proliferation and maturation, however little is known about these key cytokines in the oldest jawed vertebrates, the cartilaginous fishes. Here we report the cloning of CD40L and BAFF orthologues (designated ScCD40L and ScBAFF) in the small-spotted catshark (Scyliorhinus canicula). As predicted both proteins are type II membrane-bound proteins with a TNF homology domain in their extracellular region and both are highly expressed in shark immune tissues. ScCD40L transcript levels correlate with those of TCRα and transcription of both genes is modulated in peripheral blood leukocytes following in vitro stimulation. Although a putative CD40L orthologue was identified in the elephant shark genome the work herein is the first molecular characterisation and transcriptional analysis of CD40L in a cartilaginous fish. ScBAFF was also cloned and its transcription characterised in an attempt to resolve the discrepancies observed between spiny dogfish BAFF and bamboo shark BAFF in previously published studies.

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“…It has N-glycosylation sites (Salim et al 2003) which were detected in drusen in all pathological groups in this study.…”

Section: Discussionmentioning

confidence: 90%

Glycoproteins of drusen and drusen-like lesions

2007

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Drusen are a marker of age-related macular degeneration (AMD). Lesions similar to drusen, both in histology and their clinical appearance, are also seen in choroidal tumours, chronic inflammatory and degenerative conditions of the eye, and in mesangiocapillary glomerulonephritis type II (MCGN-II). This study aims to compare the saccharide composition of these drusen-like lesions in the various ocular pathological groups and in MCGN-II. Formalin fixed and paraffin wax embedded tissue from 21 eyes was studied. The histological diagnoses included AMD, retinal detachment, phthisis bulbi following failed retinal detachment surgery, malignant melanoma, long-standing uveitis, glaucoma and MCGN II. Glycosylation was examined using a panel of twenty biotinylated lectins and an avidin-peroxidase DAB-cobalt revealing system, with and without neuraminidase pre-treatment. High mannose, bi/tri-nonbisected and bisected complex N-glycan, N-acetyl glucosaminyl, galactosyl and sialyl residues were found to be expressed by drusen, while treatment with neuraminidase exposed subterminal N-acetyl galactosamine and galactosyl residues. Similar binding patterns were found in the various pathological groups studied. As there was no significant difference in the lectin-binding pattern in drusen in different pathologies, a common pathogenesis or at least a final common pathway for the elaboration of carbohydrate components of drusen is suggested.

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Prediction of possible sites for posttranslational modifications in human gamma crystallins: Effect of glycation on the structure of human gamma‐b‐crystallin as analyzed by molecular modeling

Cited by 10 publications

References 49 publications

B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)

B cell receptor accessory molecule CD79α: Characterisation and expression analysis in a cartilaginous fish, the spiny dogfish (Squalus acanthias)

Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark ( Scyliorhinus canicula )

Glycoproteins of drusen and drusen-like lesions

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