Prediction of retention for coupled column gradient elution high-performance liquid chromatography

Rate parameters and gradient correlations for gradient‐elution chromatography

“…While the use of gradient elution for analytical-scale chromatographic separation is well established (Freiling, 1955(Freiling, , 1957Jandera and Churacek, 1974Jandera, 1989;Snyder, 1961Snyder, , 1964Snyder et al, 1979Snyder et al, , 1989aSnyder and Glajch, 1990;Tomellini and Hartuich, 1985;Tomellini et al, 1986), few studies have addressed the basic features of adsorption kinetics and mass-transfer characteristics of industrial uses of gradient-elution chromatography.…”

Estimation of chromatographic parameters on two silica‐based columns connected in series under nonaqueous reversed phase liquid chromatographic conditions

“…It was expected from the basic chromatographic theory [25] that the retention time t R of each compound passing through the two serially linked columns would be equal to the sum of retention times t R1 + t R2 of the same compound passing through the individual columns.…”

“…Although the theory and the basic concepts of coupled columns in HPLC is well established [23,24], the practical consequences of using serially linked HPLC columns have not been pointed out explicitly [22,25,26]. Thus, several workers have used serially linked HPLC columns to achieve adequate separation without any hint on how they were led to such a choice [14 -22, 27 -30].…”