Prediction of the molecular mechanism of eucommiae Cortex - Achyranthis bidentatae radix in the treatment of osteoarthritis: network pharmacology and molecular docking

Yang, Jie; Jiao, Dijin; Zhang, Guoguang; Liu, Juntong; Qu, Chao; Chen, Hongxu; Chong-min, Chen; Yu, Sun

doi:10.1080/03639045.2021.1988098

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…There is a study [9] It shows that Radix Dipsaci can accelerate the recovery of the fracture. There are also studies [10] find: Edysterone extract of Radix Achyranthis Bidentatae may play a protective role in chondrocyte damage, β-Ecdysterone may inhibit Osteoporosis and improve bone metabolism, Achyranthes bidentata polysaccharides can significantly increase the bone mineral density, bone minerals, and trabecular bone thickness of ovariectomized rats. With the production of frankincense, myrrh, nux vomica, pseudo-ginseng to activates blood circulation and dredges channels, reduces swelling and promotes muscle growth, has the effect of removing blood stasis and promoting new blood productionIn.…”

Section: Discussionmentioning

confidence: 99%

A Case of Bone Nonunion Treated with Iliac Bone and Allogeneic Bone Grafting Combined with Jiegudan

2023

FMSR

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Fracture non-healing, also known as bone nonunion, is a common complication after fracture, but also one of the clinical problems in orthopedics. At present, there are various treatment methods for bone nonunion, and there is no perfect clinical solution. According to the specific situation of the patient, the individualized treatment plan is formulated. This paper uses the ILIAC crest and bone allograft combined with jiegudan to treat the bone nonunion, which provides a feasible idea for the setting of the clinical treatment plan.

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Section: Discussionmentioning

confidence: 99%

A Case of Bone Nonunion Treated with Iliac Bone and Allogeneic Bone Grafting Combined with Jiegudan

2023

FMSR

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“…Achyranthes bidentata is a commonly used traditional Chinese medicine that is widely used for the clinical treatment of OA. The China Food and Drug Administration has already approved numerous proprietary traditional Chinese medicines containing achyranthes bidentata , such as Mailuoning granules/injection/oral liquid and Danxi granules ( 9 ). An epidemiological survey on the use of traditional Chinese herbal medicine by patients with OA revealed that achyranthes bidentata is one of the most commonly prescribed Chinese herbs included in the Chinese herbal formula ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

“…Achyranthes bidentata has been extensively used for the treatment of OA due to its reported anti-inflammatory and antioxidant properties ( 8 , 9 ). Achyranthes bidentata polysaccharides (ABPS) is a water-soluble form of fructan that can be extracted from the Achyranthes bidentate plant ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Protective role of Achyranthes bidentata polysaccharides against chondrocyte extracellular matrix degeneration through lncRNA GAS5 in osteoarthritis

Qiu

Huang

et al. 2022

Exp Ther Med

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Achyranthes bidentata polysaccharides (ABPS) is an active ingredient of the flowering plant Achyranthes bidentata that has been previously reported to be effective for the treatment of osteoarthritis (OA). However, the underlying molecular mechanism remain to be fully clarified. Emerging studies have shown that the long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) is involved in the pathogenesis of OA. Therefore, the present study aimed to investigate the potential mechanism of ABPS by focusing on its effects on the regulation of chondrocyte extracellular matrix (ECM) homeostasis, with particular emphasis on lncRNA GAS5. In the present study, the modified Hulth method was used to construct OA rats, which were gavaged with 400 mg/kg ABPS for 8 weeks. Histopathological changes in cartilage and subchondral bone were evaluated by hematoxylin-eosin staining and Safranin O-fast green staining. In in vitro experiments, IL-1β-treated chondrocytes were infected with Lenti-lncRNA GAS5. Fluorescence in situ hybridization assay was performed to measure the expression of the lncRNA GAS5 in chondrocytes. Moreover, the relative expression level of lncRNA GAS5 in cartilage tissue and chondrocytes was detected using reverse transcription-quantitative PCR. Western blot analysis was used to detect protein expression levels of MMP-9, MMP-13, TIMP-1, TIMP-3 and type II collagen in cartilage tissue and chondrocytes. The results indicated that ABPS delayed the degradation of the ECM by chondrocytes in addition to reducing lncRNA GAS5 expression both in vivo and in vitro. Furthermore, silencing of lncRNA GAS5 expression in IL-1β-treated chondrocytes downregulated the protein expression of MMP-9 and MMP-13 whilst upregulating the expression of tissue inhibitor matrix metalloproteinase (TIMP)-1, TIMP-3 and type II collagen. To conclude, the present study provides evidence that ABPS can inhibit the expression of lncRNA GAS5 in chondrocytes to regulate the homeostasis of ECM, which in turn may delay the occurrence of cartilage degeneration during OA.

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“…It is often used to treat waist and knee pain and muscle and bone weakness (Wu et al, 2021). Our previous studies have shown that ABR extract can help improve the bone quality and trabecular structure of rats, inhibit the loss of bone mineral density, inhibit bone resorption and promote bone formation (Lu et al, 2021; Wang et al, 2021; Yang & Zeng, 2021; Yang et al, 2021). Polysaccharides, peptides, triterpene saponins, sterols and other components in ABR can regulate the immune system and enhance the reproductive ability of chondrocytes (Yang & Zeng, 2021), which has a therapeutic effect on osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

Yang

Hou

Zhang

et al. 2022

Biomedical Chromatography

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This study aimed to explore the possible mechanism of Achyranthis Bidentatae Radix for the treatment of osteoporosis using tandem mass tag-based proteomics technique combined with mass spectrometry. Proteomics techniques combined with bioinformatics were used to analyze the biological functions of differentially expressed proteins. In addition, western blotting was performed to verify the expression of related proteins. A total of 3,752 proteins were identifiable by proteomic analysis. Furthermore, 93 differentially expressed proteins were identified, of which 61 were upregulated and 32 were downregulated. Differentially expressed proteins were primarily associated with oxidative phosphorylation (p = 4.8 Â 10 À4 ) pathways and involved in transmembrane transport (p = 3.5 Â 10 À3 ), exocytic process (p = 1.2 Â 10 À2 ), cellular developmental process (p = 1.3 Â 10 À2 ), adenosine triphosphate metabolic process (p = 1.0 Â 10 À2 ) and other biological processes. Western blotting analysis showed that MT-CYB and NDUFA9 were differentially expressed in the bone microenvironment of rats with osteoporosis. We speculated that they were potential biomarkers linked to osteoporosis. This study employed proteomics to explore the potential therapeutic targets of Achyranthis Bidentatae Radix to treat osteoporosis. This revealed that mitochondria are a new target for the treatment of glucocorticoid-induced osteoporosis.

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Prediction of the molecular mechanism of eucommiae Cortex - Achyranthis bidentatae radix in the treatment of osteoarthritis: network pharmacology and molecular docking

Cited by 3 publications

References 41 publications

A Case of Bone Nonunion Treated with Iliac Bone and Allogeneic Bone Grafting Combined with Jiegudan

A Case of Bone Nonunion Treated with Iliac Bone and Allogeneic Bone Grafting Combined with Jiegudan

Protective role of Achyranthes bidentata polysaccharides against chondrocyte extracellular matrix degeneration through lncRNA GAS5 in osteoarthritis

TMT‐based proteomics analysis to screen potential biomarkers of Achyranthis Bidentatae Radix for osteoporosis in rats

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