Aqueous solutions are increasingly introduced as the working fluids of pulsating heat pipe (PHP), to balance and optimise certain thermo-physical properties of pure water. In this paper, a first attempt is conducted to predict the heat transfer performance of closed PHP with pure water and available aqueous solutions based on a fully connected feed-forward artificial neural network (ANN). The notable dimensionless numbers, Kutateladze number (Ku), Bond number (Bo), Morton number (Mo), Jackob number (Ja), Prandtl number (Pr), Laplace number (La), d/L e and number of turns (N) were selected as the inputs of the ANN model. It was found that the prediction of proposed ANN model posed a good agreement with experimental data with the MSE and correlation coefficient of 0.026 and 0.979, respectively. For 81.02% of the collected data, the absolute deviation of prediction for thermal resistance was within 25%. For 95% of collected data, the prediction of thermal resistance and temperature difference between the evaporation and condensation section fell within ±0.37 K/W, and ±17.1 K, respectively. The evaluation of evaporation and condensation section temperatures was also presented based on the proposed ANN model.