Predictive mathematical modeling for EC50 calculation of antioxidant activity and antibacterial ability of Thai bee products

Abstract

Antioxidant activities of bee products from Thailand (honey, bee pollen and propolis) via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate)(ABTS) assays were determined. The prediction of the EC50 (the half maximal effective concentration) were studied using the logistic, sigmoidal, dose response, and asymmetric 5 parameters (5P) regression models. The antimicrobial ability was tested against Staphylococcus aureus (TISTR 517), Bacillus cereus (TISTR 687), and Escherichia coli(TISTR 1261). Propolis extract with higher total phenolic content (TPC) exhibited more effective antiradical action against the DPPH and ABTS, followed by bee pollen extract and honey. All four regression models could be used to estimate the EC50 of the bee products. However, the dose-respond and 5P provide the better EC50 prediction for the bee products than the others based on the comparability of their results to those of right-angled triangle method. Thai bee products had effective antimicrobial activities on each test microorganism. The antimicrobial potency of the bee products was ranged in the order: propolis> bee pollen >honey. Results revealed that antioxidant activity and antimicrobial ability of the bee products correlated with the TPC values.

Bee pollen as a natural antioxidant source to prevent lipid oxidation in black pudding

“…The EC 50 values of bee pollen was 2.62 ± 0.09 mg/mL. This value indicates a good antioxidant activity and higher that the values reported by some authors (Negri, Barreto, Sper, Carvalho, & Campos, 2018;Suriyatem R., Auras R. A., Intipunya P., 2017).…”

In-vitro bioaccessibility of antioxidant properties of bee pollen in Turkey

“…Previous research showed that bee pollen had a high antioxidant capacity (Almedia et al, 2017;Campos et al, 2003;Suriyatem et al, 2017;Sun et al, 2017;Mărghitaş et al 2009;Carpes et al, 2007) that varies due to the ecological diversity and predominance of the plant species that it was collected from. However, there is no direct correlation available between the total phenolic content (TPC) and the antioxidant capacity (Özkök & Silici, 2017;Dorman et al, 2003;Miliauskas et al, 2004).…”

Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

“…Table 2 shows the half-maximal effective concentration (EC 50 ) of the extracts and fractions of C. nutans leaves. The EC 50 is the concentration of antioxidant that causes a 50% decrease in radical absorbance, which is commonly assessed by measuring antioxidant concentration readings [24]. Trolox, which is a well-known natural antioxidant, was used as the standard.…”

“…However, none of the crude and fraction extracts of C. nutans leaves followed the criteria of the National Cancer Institute, which requires a crude plant extract to have an IC 50 < 30 µg/mL for preliminary assay [44]. The cell viability assay was repeated using the CN-Dcm fraction extract for different incubation times (24,48, and 72 h). The IC 50 values for the three time points were plotted ( Figure 2c) and show that at 24 h of treatment, CN-Dcm inhibited 50% of cell proliferation at a concentration of 76.93 ± 0.21 µg/mL, while at 48 h the IC 50 of the extract was 74.38 ± 0.19 µg/mL and at 72 h it was 65.95 ± 0.14 µg/mL.…”

“…The extract with highest SI CN-Dcm) was selected for another cell viability assay at different incubation times (24,48, and 72 h).…”