Predictive models for nanotoxicology: in vitro, in vivo, and computational models

Pimtong, Wittaya; Samutrtai, Pawitrabhorn; Wongwanakul, Ratjika; Aueviriyavit, Sasitorn

doi:10.1016/b978-0-12-821506-7.00026-0

Cited by 5 publications

(8 citation statements)

References 122 publications

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“…While the costs increase with the complexity of in vitro systems, the results obtained using these systems are also more informative. 60 Considering that in vitro tests can be both high-content and high-throughput, their application offers the possibility to test many more nanomaterials and Environmental Science: Nano Perspective also react faster to the development of new materials than what would be possible using only animals. 61,62 Being able to do more tests in less time also means, with respect to the toxicity effect factor calculation, that different cell lines could be used to test both the inhalation and the ingestion exposure routes, avoiding the need for route-toroute extrapolation.…”

Section: Existing Toxicity Effect Factors For Nanomaterialsmentioning

confidence: 99%

“…72 By combining living cell tissues (that can go from simple 2D cultures to complex organoids) with a microfluidic system, the organ-on-a-chip creates a physiological microenvironment where the complex responses to stimuli or substances can be monitored. 73,74 There is therefore great potential for in vitro tests to better mimic human responses, even though the current costs and complexity of these advanced systems limit their systematic application, 60,74 making the use of these data difficult in the LCIA context. Moreover, additional work is needed to verify the predictivity and reliability of these technologies, 63 and until then LCIA should prefer to extrapolate human toxicity from animal data (if available).…”

Section: Existing Toxicity Effect Factors For Nanomaterialsmentioning

confidence: 99%

“…More and more used, organoids are 3D multicellular in vitro systems in which stem cells organize and differentiate into complex tissue structures, thus mimicking specific organs. 60,70,71 Recognized by the World Economic Forum as a top emerging technology in 2016, organs-on-a-chip allow a level of emulation of biological systems never seen before. 72 By combining living cell tissues (that can go from simple 2D cultures to complex organoids) with a microfluidic system, the organ-on-a-chip creates a physiological microenvironment where the complex responses to stimuli or substances can be monitored.…”

Section: Challenges and Advantages Of The Use Of In Vitro Datamentioning

confidence: 99%

Section: Challenges and Advantages Of The Use Of In Vitro Datamentioning

confidence: 99%

See 3 more Smart Citations

In vitro-based human toxicity effect factors: challenges and opportunities for nanomaterial impact assessment

Romeo

Hischier

Nowack

et al. 2022

Environ. Sci.: Nano

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Section: Existing Toxicity Effect Factors For Nanomaterialsmentioning

confidence: 99%

Section: Existing Toxicity Effect Factors For Nanomaterialsmentioning

confidence: 99%

Section: Challenges and Advantages Of The Use Of In Vitro Datamentioning

confidence: 99%

Section: Challenges and Advantages Of The Use Of In Vitro Datamentioning

confidence: 99%

See 2 more Smart Citations

In vitro-based human toxicity effect factors: challenges and opportunities for nanomaterial impact assessment

Romeo

Hischier

Nowack

et al. 2022

Environ. Sci.: Nano

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show abstract

“…On the other hand, many studies have revealed the presence of toxic effects of nanoparticles in vivo (7) . There is a special modern scientific branch that aims to study the potential negative effects of nanoparticles and related parameters affecting the cytotoxicity of nanomaterial's called nanotoxicology (8,9) . To this day, herbs are mainly used as treatment in many developing countries for primary health care due to their acceptability in the human body as well as their fewer side effects (10,11) .…”

Section: Introductionmentioning

confidence: 99%

Toxicity of AgNPs upon Liver Function and Positive Role of Tinospora Cordifolia: In Vivo

Ramadhan¹,

Ghareeb²

2021

PJMHS

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This experiment was premeditated to probe the toxic impact of Silver Nanoparticles (AgNPs) upon liver function parameters in male rats, as well as to demonstrate the protective effect of Tinospora cordifolia (T.C) against liver function disorders caused by AgNPs. Twenty-four rats were classified into four packs, six ones for every pack. Rats were included by control animals without treatment, while the AgNPs group included rats treated with 50μl/kg / day AgNPs. In the AgNPs + T.C group, rats were co-administered of AgNPs at a dose of 50μl/kg with T.C at a dose of 400 mg/kg. Whereas, animals of the T.C pack were treated with 400 mg/kg of T.C. The duration of the experiment was 28 days. After blood samples were taken at the conclusion of the experiment. The levels of biochemical parameters (liver enzymes) such as alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were evaluated to detect liver function abnormalities. Thwe Pw A t e dewoe t ste user e dnoAiwoe se dsnd disnte dnirease in the activity of serum liver enzymes in comparison to the control group (p < 0.05).While T. cordifolia co-treatment significantly reduced the levels of activities of those enzymes. Therefore, it can be considered that T. cordifolia effectively contributes to the protection against liver dysfunction caused by AgNPs in rats. Keywords : Silver Nanoparticles, liver dysfunction, biochemical parameters.

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Toxicity of carbon nanomaterials in Caenorhabditis elegans: Effects of biocorona formation on single and co-exposure testing

Côa

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This thesis aimed to evaluate the effects of biocorona formation on carbon nanomaterials (i.e., graphene oxide -GO and multiwalled carbon nanotubes -MWCNT) toxicity in the nematode Caenorhabditis elegans model. In the first chapter, a literature review introduced this organism as a valuable model for nanotoxicity research; focusing on carbon nanomaterials toxicity, nanobiointerfaces, and discussing experimental gaps towards harmonization of protocols and best laboratory practices. In the second chapter, bovine serum albumin was exploited as a protein model to investigate the implications of biocorona formation on the toxicity of GO and MWCNT. This study demonstrated that both materials were toxic to nematode survival, growth, reproduction, and fertility, as well as enhanced oxidative stress and permeability of intestinal barrier. They crossed the nematode intestine reaching its secondary organs, but albumin corona hindered the MWCNT translocation, while coated GO was translocated affecting the functionality of crucial organs. As consequence, the effects of GO were 50% attenuated by protein coating, while damages of MWCNT were 100% mitigated. In the third chapter, the combined toxicity of GO and silver nitrate (Ag + ) in co-exposure testing was analysed, and the concept of E. coli corona formation was addressed for the first time in the literature. Results suggest that GO increased the lethality of Ag + 2.1 times, but it was aggravated 4.6 times when GO was coated by E. coli corona. Bare and coated GO were translocated to secondary organs of C. elegans, where they promoted the enhancement of apoptotic corpses in germline and potential injuries to neurons. Therefore, these results suggest that assessing the implications of E. coli corona formation to nanomaterials toxicity must be a priority in the assays with C. elegans. Finally, this thesis provides a fundamental understanding of biocorona role and reinforces the importance of considering its influence on nanomaterials toxicity towards a safer applications of carbon nanomaterials. Keywords: Nanotoxicology. Protein corona. Combined toxicity. Nanosafety. RESUMOCÔA, F. Toxicidade de nanomateriais de carbono no Caenorhabditis elegans: efeitos da formação da biocorona em ensaios de exposição simples e combinada. 2023. 164 p. Tese (Doutorado em Ciências) -

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Predictive models for nanotoxicology: in vitro, in vivo, and computational models

Cited by 5 publications

References 122 publications

In vitro-based human toxicity effect factors: challenges and opportunities for nanomaterial impact assessment

In vitro-based human toxicity effect factors: challenges and opportunities for nanomaterial impact assessment

Toxicity of AgNPs upon Liver Function and Positive Role of Tinospora Cordifolia: In Vivo

Toxicity of carbon nanomaterials in Caenorhabditis elegans: Effects of biocorona formation on single and co-exposure testing

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