Predictive value for lymph node metastasis of epithelial-mesenchymal transition and cancer stem cell marker expression in early gastric cancer

Nam, Kyung Han; Yoon, Hyuk; Lee, Kyungbun; Park, Do Joong; Kim, Hyung‐Ho; Lee, Hye Seung; Shin, Eun Hee

doi:10.1016/j.prp.2017.03.010

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…We suppose that high CD28 expression may be due to CD4 + T-cell activation related to micrometastases in LNs in lung cancer patients and may control the metastatic cells in these compartments. A low frequency of stemness markers was described in LNs in gastric cancer patients and was an independent predictive factor for LNs metastasis [22].…”

Section: Discussionmentioning

confidence: 95%

PD-L1+ lung cancer stem cells modify the metastatic lymph-node immunomicroenvironment in nsclc patients

Raniszewska

Vroman

Dumoulin

et al. 2020

Cancer Immunol Immunother

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Introduction Cancer stem cells (CSCs) are implicated in tumor initiation and development of metastasis. However, whether CSCs also affect the immune system is not fully understood. We investigated correlations between the PD-L1 + CSCs, changes in T-cell phenotype in metastatic and non-metastatic lymph nodes (LNs) and response to treatment. Methods LNs' aspirates were obtained during the EBUS/TBNA procedure of 20 NSCLC patients at different stages of the disease. CSCs and T-cell characteristics were determined by flow cytometry. Results PD-L1 + CSCs positively correlated with the percentage of Tregs, PD-1 + CD4 T cells and Tim3 + CD4 + T cells, whereas PD-L1 + CSCs were negatively correlated with CD4 + T cells and CD28 + CD4 + T cells. The percentage of PD-L1 + CSCs was higher in patients with progressive disease (PD) as compared to patients with stable disease (SD) or partial response (PR). Among T cells, only PD-1 + CD4 + T cells and Tim3 + CD4 + T-cell frequencies were higher in patients with PD as compared to patients with SD or PR. Conclusion The frequency of PD-L1 + CSCs associates with an altered T-cell frequency and phenotype indicating that CSCs can affect the immune system. The higher percentage of PD-L1 + CSCs in patients with PD may confirm their resistance to conventional therapy, suggesting that CSCs may be an interesting target for immunotherapy.

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Section: Discussionmentioning

confidence: 95%

PD-L1+ lung cancer stem cells modify the metastatic lymph-node immunomicroenvironment in nsclc patients

Raniszewska

Vroman

Dumoulin

et al. 2020

Cancer Immunol Immunother

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“…For example, Ouban et al (12) found through analysis on the carcinoma and adjacent normal tissues of 50 patients with breast cancer that the expression of Claudin-1 significantly declined in carcinoma tissues, and it had significant negative correlations with tumor size, lymph node metastasis and tumor stage. Moldvay (4) and Nam et al (13) found that the high expression of Claudin-1 indicated a good prognosis of non-small cell lung cancer and colorectal cancer. Liu et al (5) confirmed that the expression of Claudin-1 was inhibited by ZIP4 in pancreatic cancer, thus facilitating invasion and metastasis of pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration

She

Cai

2022

Revista Romana De Medicina De Laborator

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Background: Claudin-1 is involved in various cancers, but its expression and role in gastric carcinoma remain unclear. Materials and Methods: Gastric carcinoma and adjacent normal tissues were harvested from 60 patients. Claudin-1 expression was detected by RT-qPCR. The expressions in human gastric carcinoma MKN45, SGC7901 and MKN28 cells and immortalized human gastric epithelium GES-1 cells were determined by RT-qPCR and Western blotting. Claudin-1 was overexpressed in SGC7901 cells by lentiviral transfection, and they were divided into Control (untransfected), normal control (NC) (transfected with lentiviral vector) and Claudin-1 (transfected with Claudin-1 overexpression lentivirus) groups. The proliferation, invasion and migration of gastric carcinoma cells were detected through cell counting kit-8, Transwell and wound healing assays, respectively. The effects of Claudin-1 on the expressions of epithelial-mesenchymal transition (EMT) marker proteins E-cadherin and N-cadherin were detected by Western blotting. Ten 4-week-old male BALB/c nude mice were subcutaneously injected with lentivirus-treated SGC7901 cells to establish the transplanted tumor model, and the effect of overexpression of Claudin-1 was explored. Results: The expression of Claudin-1 in gastric carcinoma tissues was significantly lower than that in adjacent tissues (P<0.05). Overexpression of Claudin-1 significantly inhibited the proliferation, invasion and migration of SGC7901 cells, increased the expression of E-cadherin, and decreased that of N-cadherin (P<0.05). Overexpression of Claudin-1 in the mouse model significantly inhibited the growth of subcutaneous transplanted tumors (P<0.05). Conclusion: Claudin-1 has low expression in gastric carcinoma tissues. Overexpression of Claudin-1 inhibits the proliferation, invasion, migration, and EMT of gastric carcinoma cells, and subcutaneous tumorigenesis in nude mice.

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“…E-cadherin and P-cadherin are transmembrane glycoproteins localized in the adherent junctions of epithelial cells ( 14 ). The membrane staining of E-cadherin correlates with lymph node metastasis ( 15 , 16 , 30 ). EpCAM is also a transmembrane glycoprotein that mediates Ca 2+ -independent homotypic cell-cell adhesion ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Predictive value of E-cadherin and EpCAM for detection of metastatic lymph node in early gastric cancer

Eom

Ryu

Yoon

et al. 2020

Chinese Journal of Cancer Research

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Objective There has been a demand for a tumor-specific marker for metastatic lymph nodes in sentinel navigation surgery for gastric cancer. The aim of this study is to analyze protein expression in both primary tumors and metastatic lymph nodes in early gastric cancer patients. Methods We collected primary tumors and metastatic lymph nodes from 71 patients who underwent curative gastrectomy and pathologically diagnosed with T1N1 or T1N2 (8th Union for International Cancer Control 8th edition/American Joint Committee on Cancer staging system) gastric cancer. Immunohistochemistry was used to determine the expression of six cell membrane proteins, including carcinoembryonic antigen (CEA), E-cadherin, epithelial cell adhesion molecule (EpCAM), P-cadherin, CD44v6, and c-erbB2 in the patient samples. Results The expression of CEA, E-cadherin, EpCAM, P-cadherin, CD44v6 and c-erbB2 in the evaluable primary tumor samples was 75.4%, 97.1%, 100%, 89.9%, 11.1% and 7.2%, respectively. Among cases wherein both the primary tumor and metastatic lymph nodes were evaluable, double positivity (expression in both primary tumor and metastatic lymph nodes) was observed for CEA, E-cadherin, EpCAM, P-cadherin, CD44v6 and c-erbB2 in 53.2%, 97.9%, 98.1%, 76.6%, 0 and 6.8% of the cases, respectively. The proportion of metastatic lymph nodes positive for CEA, E-cadherin, EpCAM, P-cadherin, CD44v6 and c-erbB2 was 71.4%, 100%, 98.1%, 83.7%, 0, and 75%, respectively in primary tumors positive for the same markers. Conclusions E-cadherin and EpCAM had an overlap of 100% and 98.1% between the primary tumor and metastatic lymph nodes, respectively. Thus, E-cadherin and EpCAM are potential molecular markers to detect metastatic lymph nodes in patients with early gastric cancer.

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Predictive value for lymph node metastasis of epithelial-mesenchymal transition and cancer stem cell marker expression in early gastric cancer

Cited by 9 publications

References 27 publications

PD-L1+ lung cancer stem cells modify the metastatic lymph-node immunomicroenvironment in nsclc patients

PD-L1+ lung cancer stem cells modify the metastatic lymph-node immunomicroenvironment in nsclc patients

Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration

Predictive value of E-cadherin and EpCAM for detection of metastatic lymph node in early gastric cancer

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