Predominance of Candida Glabrata among Non-albicans Candida Species in a 16-Year Study of Candidemia at a Tertiary Care Center in Lebanon

Zakhem, Aline El; Istambouli, Rachid; Alkozah, Maria; Gharamti, Amal; Tfaily, Mohamad Ali; Jabbour, Jean-Francois; Araj, George F.; Tamim, Hani; Kanj, Souha S.

doi:10.3390/pathogens10010082

Cited by 20 publications

(26 citation statements)

References 35 publications

(53 reference statements)

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“…These results were in agreement with another study showed that C. glabrata was the most prevalent Candida species etiology of candiduria (50%) in diabetic patients, followed by C. albicans (31.6%) and C. krusei (10.5%) (Falahati et al, 2016). Furthermore, the study of Zakhem et al (2021) showed C. glabrata as the predominant species among non-albicans Candida in candidemia cases. Recent findings (Sahai and Kumar, 2018;Pramodhini et al, 2021) have also been detected that non-albicans Candida species were predominant in urinary tract infections.…”

Section: Discussionsupporting

confidence: 91%

Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women

Ibrahim¹,

Hamzah²

2021

JLBSR

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Biofilms are multicellular communities where microorganisms are grown and form an extracellular matrix that protects the pathogenic microorganisms from the immunity system and antimicrobial agents. This study is aimed to identify Candida spp. isolated from urine specimens by using traditional techniques, germ tube, growth on corn meal agar medium and chromogenic medium then determine the ability of isolates to producing biofilm by tube method (TM) and congo red agar method (CRA). In our study urine specimens were obtained from 174 diabetic females in the period of six months at the Al-Wafa Specialized Center for Diabetes and Endocrinology, Mosul city, Iraq. Out of the total 174 specimens, yeast species were isolated from 56 (32.2 %) specimens. Out of the 56 isolates, 50 isolates were positive for Candida spp., especially C. glabrata which appeared maximum in 30 isolates (60 %) and followed by C. albicans 18 isolates (36%) and C. krusei 2 isolates (4 %). In the TM method for biofilm detection, C. albicans showed 16 isolates (88.9%) positive for biofilm formation followed by 29 isolates (96.7%) of C. glabrata. Furthermore, in the CRA method, all isolates (100%) of C. albicans were negative followed by 27 isolates (90%) of C. glabrata, whereas only 3 isolates (10%) of C. glabrata were positive. We can conclude that TM is the best conventional method and is sensitive to detect biofilm-forming yeast when compared with the CRA method.

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Section: Discussionsupporting

confidence: 91%

Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women

Ibrahim¹,

Hamzah²

2021

JLBSR

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“…Regarding genus, there was no difference between those that were more frequent indoor and outdoor, and in both samples, Penicillium, Cladosporium, and Aspergillus were the most frequent. These findings align with Ziaee et al (2018), who also verified this genus as the most frequent in their samples. Regarding fungal species, few species were identified only in outdoor air.…”

supporting

confidence: 88%

“…The occurrence of nonalbicans Candida in critical areas is worrying when we consider the compromised immunological condition of patients and the fact that some species are resistant to the antifungal agents currently used in therapy. In line with this, Gong et al (2016) and Zakhem et al (2021) reported non-albicans Candida as the causal agent of invasive candidiasis and candidemia, respectively. In their papers, the authors described C. tropicalis and C. parapsilosis as the causative agent of the infections, and we verified these yeasts in our samples.…”

Section: Discussionsupporting

confidence: 64%

Airborne Fungi in a Neonatal Intensive Care Unit and Operating Theater in a University Hospital

Pedrosa

Nascimento

Araújo³

et al. 2022

Int J Innov Educ Res

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Monitoring the microbiological quality of indoor air in hospital environments is a matter of comprehensive discussion due to its influence on the transmission and spread of pathogenic microorganisms. Among the artificially air-conditioned environments, hospitals are noteworthy for being specific places for the treatment and recovery of patients. In addition to problems related to patients health and professionals health, immunocompromised patients are more exposed to microorganisms present in the air currents of the refrigeration system in these environments, which can lead to consequences such as the occurrence of outbreaks. The objective of this work was to evaluate the indoor air quality in critical hospital environments of a teaching hospital in the city of Maceió, the state of Alagoas. In addition, we sought to identify the anemophilous fungal microbiota present. Air collections were taken in the rainy season, totaling, following recommendations indicated by Resolution No. 9 of the Brazilian National Health Surveillance Agency. The study was based on determining the concentration parameter of bioaerosols in indoor and outdoor air. The fungal microbiota identification was carried out by analyzing macro and microscopic characteristics for filamentous fungi and the use of molecular tools for yeasts. The most frequent species in hospital critical environments were Cladosporium cladosporioides, Penicillium piceum, Penicillium aurantiogriseum, Cladosporium herbarum and Aspergillus oryzae. In outdoor air, the most frequently found fungi were Penicillium sp., Aspergillus sp., and Cladosporium species. Candida tropicalis, C. krusei, and C. parapsilosis were identified among the yeasts in indoor and outdoor air samples. Identifying potentially pathogenic fungi in the evaluated environments points to the need for continuous monitoring of indoor air quality. Furthermore, to avoid the widespread fungal pathogens and the consequent occurrence of outbreaks, the adoption of indoor air microbiological quality analysis programs is suggested as an essential tool in developing infection control standards. In our study, airborne fungi are reported as indoor air contaminants in critical hospital environments. This finding is noteworthy because, in general, individuals present in these environments have an immunological impairment.

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“…Candida albicans is the most frequently isolated species associated with oral candidosis, accounting for between 60% and 75% [2,3] of cases and involving between 41% and 30% of candidemia episodes. However, over the last few years, non-albicans Candida strains, responsible for oral candidosis, have tended to increase, and together they cause the majority of Candida bloodstream infections, even though with important differences across countries and continents [4][5][6][7]. Recent epidemiological studies have reported an increased incidence of 2 of 12 non-albicans candidemia, especially in the USA, Northern Europe, and Australia, due to Candida glabrata and Candida tropicalis, which are less susceptible to antifungal drugs and could be associated with high morbidity and high mortality [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis

Mancino

Kharouf

Scavello

et al. 2022

IJMS

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Resistance to antifungal therapy of Candida albicans and non-albicans Candida strains, frequently associated with oral candidosis, is on the rise. In this context, host-defense peptides have emerged as new promising candidates to overcome antifungal resistance. Thus, the aim of this study was to assess the effectiveness against Candida species of different Catestatin-derived peptides, as well as the combined effect with serum albumin. Among Catestatin-derived peptides, the most active against sensitive and resistant strains of C. albicans, C. tropicalis and C. glabrata was the D-isomer of Cateslytin (D-bCtl) whereas the efficiency of the L-isomer (L-bCtl) significantly decreases against C. glabrata strains. Images obtained by transmission electron microscopy clearly demonstrated fungal membrane lysis and the leakage of the intracellular material induced by the L-bCtl and D-bCtl peptides. The possible synergistic effect of albumin on Catestatin-derived peptides activity was investigated too. Our finding showed that bovine serum albumin (BSA) when combined with the L- isomer of Catestatin (L-bCts) had a synergistic effect against Candida albicans especially at low concentrations of BSA; however, no synergistic effect was detected when BSA interacted with L-bCtl, suggesting the importance of the C-terminal end of L-bCts (GPGLQL) for the interaction with BSA. In this context in vitro D-bCtl, as well as the combination of BSA with L-bCts are potential candidates for the development of new antifungal drugs for the treatment of oral candidosis due to Candida and non-Candida albicans, without detrimental side effects.

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Predominance of Candida Glabrata among Non-albicans Candida Species in a 16-Year Study of Candidemia at a Tertiary Care Center in Lebanon

Cited by 20 publications

References 35 publications

Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women

Biofilm formation by Candida albicans and Candida glabrata isolated from urine specimens of diabetic Iraqi women

Airborne Fungi in a Neonatal Intensive Care Unit and Operating Theater in a University Hospital

The Catestatin-Derived Peptides Are New Actors to Fight the Development of Oral Candidosis

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