Predominant cause of faster force recovery in females than males after intense eccentric contractions in mouse fast‐twitch muscle

Watanabe, Daiki; Ikegami, Ryo; Kano, Yutaka

doi:10.1113/jp281927

Cited by 5 publications

(2 citation statements)

References 53 publications

(130 reference statements)

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“…Our values of torque as a function of frequency, angular velocity and power are within ranges reported previously for the mouse plantar flexors in vivo (Baltgalvis et al, 2012;Hamm et al, 2021;Song et al, 2019;Watanabe et al, 2021;Wenke et al, 2010). As well, our values of tetanic [Ca 2+ ] i are within ranges reported previously from mouse FDB intact fibres (Chin et al, 2014).…”

Section: Discussionsupporting

confidence: 90%

“…Eight weeks following removal of the ovaries, Watanabe et al. ( 2021 ) observed no differences in the plantar flexor torque–frequency relationship between OVX mice, controls and OVX mice that received 17β‐estradiol. Others have also observed no differences in maximum isometric dorsiflexion torque between control and OVX mice 8 weeks following removal of the ovaries (Kosir et al., 2015 ; Vang et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Hinks,

Dalton,

Mashouri

et al. 2024

Experimental Physiology

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The abrupt cessation of ovarian hormone release is associated with declines in muscle contractile function, yet the impact of gradual ovarian failure on muscle contractility across peri‐, early‐ and late‐stage menopause remains unclear. In this study, a 4‐vinylcyclohexene diepoxide (VCD)‐induced ovarian failure mouse model was used to examine time course changes in muscle mechanical function. Plantar flexors of female mice (VCD: n = 10; CON: n = 8) were assessed at 40 (early perimenopause), 80 (late perimenopause), 120 (menopause onset) and 176 (late menopause) days post‐initial VCD injection. A torque–frequency relationship was established across a range of frequencies (10–200 Hz). Isotonic dynamic contractions were elicited against relative loads (10–80% maximal isometric torque) to determine the torque–velocity–power relationship. Mice then performed a fatigue task using intermittent 100 Hz isometric contractions until torque dropped by 60%. Recovery of twitch, 10 Hz and 100 Hz torque were tracked for 10 min post‐task failure. Additionally, intact muscle fibres from the flexor digitorum brevis underwent a fatigue task (50 repetitions at 70 Hz), and 10 and 100 Hz tetanic [Ca2+] were monitored for 10 min afterward. VCD mice exhibited 16% lower twitch torque than controls across all time points. Apart from twitch torque, 10 Hz torque and 10 Hz tetanic [Ca2+], where VCD showed greater values relative to pre‐fatigue during recovery, no significant differences were observed between control and VCD mice during recovery. These results indicate that gradual ovarian failure has minimal detriments to in vivo muscle mechanical function, with minor alterations observed primarily for low‐frequency stimulation during recovery from fatigue.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Hinks,

Dalton,

Mashouri

et al. 2024

Experimental Physiology

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Ca²⁺ storage function is altered in the sarcoplasmic reticulum of skeletal muscle lacking mitsugumin 23

Watanabe,

Nishi,

Liu

et al. 2024

American Journal of Physiology-Cell Physiology

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Mitsugumin 23 (MG23) has been identified as a ball-shaped cation channel in the sarcoplasmic reticulum (SR) but its physiological role remains unclear. This study aimed to examine the contribution of MG23 to Ca2+ storage function in skeletal muscle by using Mg23-knockout ( Mg23-/-) mice. There was no difference in the isometric specific force of the extensor digitorum longus (EDL) and soleus (SOL) muscles between Mg23-/- and wild-type (Wt) mice. In Mg23-/- mice, the calsequestrin 2 content in the EDL muscle and SR Ca2+-ATPase 2 content in the SOL were increased. We have examined SR and myofibril functions using mechanically skinned fibers and determined their fiber types based on the response to Sr2+, which showed that Mg23-/- mice, compared to Wt, had: i) elevated total Ca2+ content in the membranous components including SR, mitochondria and transverse tubular system, referred to as endogenous Ca2+ content, in both type I and II fibers of the EDL and SOL; ii) increased maximal Ca2+ content in both type I and II fibers of the EDL and SOL; iii) decreased SR Ca2+ leakage in type I fibers of the SOL; and iv) enhanced SR Ca2+ uptake in type I fibers of the SOL, although myofibril function was not different in both type I and II fibers of the SOL and EDL muscles. These results suggest that MG23 decreases SR Ca2+ storage in both type I and type II fibers, likely due to increased SR Ca2+ leakage.

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Glutathione depression alters cellular mechanisms of skeletal muscle fatigue in early stage of recovery and prolongs force depression in late stage of recovery

Watanabe

Wada

2023

American Journal of Physiology-Regulatory, Integrative and Comp

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The effects of reduced glutathione (GSH) on skeletal muscle fatigue were investigated. GSH was depressed by buthionine sulfoximine (BSO) (100 mg/kg body weight/day) treatment for 5 d, which decreased GSH content to ~10%. Male Wistar rats were assigned to the control ( N = 18) and BSO groups ( N =17). Twelve hours after BSO treatment, the plantar flexor muscles were subjected to fatiguing stimulation (FS). Eight control and seven BSO rats were rested for 0.5 h (early stage of recovery), and the remaining were rested for 6 h (late stage of recovery). Forces were measured before FS and after rest, and physiological functions were estimated using mechanically skinned fibers. The force at 40 Hz decreased to a similar extent in both groups in the early stage of recovery and was restored in the control but not in the BSO group in the late stage of recovery. In the early stage of recovery, sarcoplasmic reticulum (SR) Ca2+ release was decreased in the control greater than in the BSO group, whereas myofibrillar Ca2+ sensitivity was increased in the control but not in the BSO group. In the late stage of recovery, SR Ca2+ release decreased and SR Ca2+ leakage increased in the BSO group but not in the control group. These results indicate that GSH depression alters the cellular mechanism of muscle fatigue in the early stage and delays force recovery in the late stage of recovery, due at least in part, to the prolonged Ca2+ leakage from the SR.

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Predominant cause of faster force recovery in females than males after intense eccentric contractions in mouse fast‐twitch muscle

Abstract: support-information-section).

Cited by 5 publications

References 53 publications

Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Ca²⁺ storage function is altered in the sarcoplasmic reticulum of skeletal muscle lacking mitsugumin 23

Glutathione depression alters cellular mechanisms of skeletal muscle fatigue in early stage of recovery and prolongs force depression in late stage of recovery

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Predominant cause of faster force recovery in females than males after intense eccentric contractions in mouse fast‐twitch muscle

Abstract: support-information-section).

Cited by 5 publications

References 53 publications

Time course changes in in vivo muscle mechanical function and Ca2+ regulation of force following experimentally induced gradual ovarian failure in mice

Time course changes in in vivo muscle mechanical function and Ca2+ regulation of force following experimentally induced gradual ovarian failure in mice

Ca2+ storage function is altered in the sarcoplasmic reticulum of skeletal muscle lacking mitsugumin 23

Glutathione depression alters cellular mechanisms of skeletal muscle fatigue in early stage of recovery and prolongs force depression in late stage of recovery

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Product

Resources

About

Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Time course changes in in vivo muscle mechanical function and Ca²⁺ regulation of force following experimentally induced gradual ovarian failure in mice

Ca²⁺ storage function is altered in the sarcoplasmic reticulum of skeletal muscle lacking mitsugumin 23