Preeclampsia: inflammatory signature of decidual cells in early manifestation of disease

Vishnyakova, Polina; Poltavets, Anastasiya; Nikitina, Maria; Muminova, Muminova К.Т.; Potapova, Alena; Вторушина, В В; Loginova, N. S.; Midiber, Konstantin; Mikhaleva, Liudmila M.; Lokhonina, Anastasia; Ходжаева, З.С.; Pyregov, Pyregov A.V.; Elchaninov, Andrey; Fatkhudinov, Timur; Сухих, Г. Т.

doi:10.1016/j.placenta.2021.01.011

Cited by 18 publications

(10 citation statements)

References 59 publications

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“…PE continues to be the leading cause of maternal and perinatal mortality and morbidity worldwide. The involvement of the monocyte–macrophage system in PE was confirmed by many experimental studies demonstrating an increase in pro-inflammatory and a decrease in anti-inflammatory macrophage numbers in the placenta, both fetal and maternal parts of the organ 10 , 12 – 16 . A possible role of blood monocytes as macrophage precursors in PE remains an open issue.…”

Section: Discussionmentioning

confidence: 82%

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia

Polina

Кузнецова

Anastasiya

et al. 2022

Sci Rep

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Preeclampsia (PE) is a serious gestational complication affecting the life of a mother and child. The immunophenotype and gene expression profile of isolated blood monocyte subpopulations of pregnant women with PE have not been studied before. In this work, we assessed changes in CD14++ and CD16++ monocyte subpopulations in PE and physiological pregnancy (n = 33). Immunophenotyping, immunomagnetic sorting of monocytes and analysis of the transcriptional profile of their genes were carried out. The percentage of classical monocytes was significantly lower, while the intermediate fraction of monocytes was significantly higher in late-onset PE compared to control. Transcriptome analysis of late-onset PE classical CD14++ monocytes revealed significant activation of inflammation mediated by chemokine and cytokine signalling pathways; apoptosis; regulation of transcription from RNA polymerase II promoter in response to stress and others. The most suppressed signalling pathways were associated with T cell activation and selection. In CD16++ monocytes of late-onset PE cases, positive regulation of cell–cell adhesion, integrin signalling pathway, blood coagulation cascade were the most activated ones. The inflammation mediated by chemokine and cytokine signalling pathway and p53 pathway were the most down-regulated in CD16++ monocytes. The obtained results indicate profound changes occurring to two most polar monocyte subpopulations in PE and their different roles in the pathogenesis of this disease.

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Section: Discussionmentioning

confidence: 82%

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia

Polina

Кузнецова

Anastasiya

et al. 2022

Sci Rep

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“…However, we achieved to establish an isolation method enabling the comparison of decidual macrophages and Hofbauer cells from term placenta in a, as less as possible activated and differently influenced form, there are limitations left to be discussed. Even though purification of our cell population was verified against trophoblasts, the purity relative to other leucocytes, as performed by other groups [ 83 ], would be interesting to be analyzed. Furthermore, analysis of CD163 and CD80 is not sufficient to make an exact statement of M1- or M2-like polarization, which was not the aim of this study so far, but was performed to demonstrate that by using our method macrophage polarization analysis might be one possible application purpose.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta—Comparison of the Expression of CD163 and CD80

Lasch

Sudan

Paul

et al. 2022

IJMS

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(1) Background: Placental immune cells are playing a very important role in a successful placentation and the prevention of pregnancy complications. Macrophages dominate in number and relevance in the maternal and the fetal part of the placenta. The evidence on the polarization state of fetal and maternal macrophages involved in both, healthy and pregnancy-associated diseases, is limited. There is no representative isolation method for the direct comparison of maternal and fetal macrophages so far. (2) Material and Methods: For the isolation of decidual macrophages and Hofbauer cells from term placenta, fresh tissue was mechanically dissected and digested with trypsin and collagenase A. Afterwards cell enrichment was increased by a Percoll gradient. CD68 is represented as pan-macrophage marker, the surface markers CD80 and CD163 were further investigated. (3) Results: The established method revealed a high cell yield and purity of the isolated macrophages and enabled the comparison between decidual macrophages and Hofbauer cells. No significant difference was observed in the percentage of single CD163+ cells in the distinct macrophage populations, by using FACS and immunofluorescence staining. A slight increase of CD80+ cells could be found in the decidual macrophages. Considering the percentage of CD80+CD163− and CD80−CD163+ cells we could not find differences. Interestingly we found an increased number of double positive cells (CD80+CD163+) in the decidual macrophage population in comparison to Hofbauer cells. (4) Conclusion: In this study we demonstrate that our established isolation method enables the investigation of decidual macrophages and Hofbauer cells in the placenta. It represents a promising method for direct cell comparison, enzyme independently, and unaffected by magnetic beads, to understand the functional subsets of placental macrophages and to identify therapeutic targets of pregnancy associated diseases.

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“…There is a huge variety of markers used for the identification of macrophage population. 44–54 Examples of surface marker detection by flow cytometry are shown below:…”

Section: M1/m2 Paradigm Of Macrophage Polarizationmentioning

confidence: 99%

“…43 There is a huge variety of markers used for the identification of macrophage population. [44][45][46][47][48][49][50][51][52][53][54] Examples of surface marker detection by flow cytometry are shown below: 54 Of note, CD45 + , CD14 + , CD11b + , CD163 + , CD19, and CD68 + are common markers for human whole-population macrophages.…”

Section: M1/m2 Paradigm Of Macrophage Polarizationmentioning

confidence: 99%

Macrophage polarization in hypoxia and ischemia/reperfusion: Insights into the role of energetic metabolism

Yakupova

Maleev

Krivtsov

et al. 2022

Exp Biol Med (Maywood)

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Macrophages, the key cells of innate immunity, possess wide phenotypical and functional heterogeneity. In vitro studies showed that microenvironment signals could induce the so-called polarization of macrophages into two phenotypes: classically activated macrophages (M1) or alternatively activated macrophages (M2). Functionally, they are considered as proinflammatory and anti-inflammatory/pro-regenerative, respectively. However, in vivo studies into macrophage states revealed a continuum of phenotypes from M1 to M2 state instead of the clearly distinguished extreme phenotypes. An important role in determining the type of polarization of macrophages is played by energy metabolism, including the activity of oxidative phosphorylation. In this regard, hypoxia and ischemia that affect cellular energetics can modulate macrophage polarization. Here, we overview the data on macrophage polarization during metabolic shift–associated pathologies including ischemia and ischemia/reperfusion in various organs and discuss the role of energy metabolism potentially triggering the macrophage polarization.

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Preeclampsia: inflammatory signature of decidual cells in early manifestation of disease

Cited by 18 publications

References 59 publications

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia

Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia

Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta—Comparison of the Expression of CD163 and CD80

Macrophage polarization in hypoxia and ischemia/reperfusion: Insights into the role of energetic metabolism

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