Preface: Happy 50th anniversary!

Plebani, Mario

doi:10.1515/cclm-2012-0333

Cited by 20 publications

(28 citation statements)

References 4 publications

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“…However, its specificity for GPA is obviously lower than that of the antigen-specific PR3-ANCA assays. Thus, the combination of both IIF and antigen-specific assays was confirmed in several studies to be the optimal strategy for ANCA detection [33].…”

Section: Discussionmentioning

confidence: 72%

“…Damoiseaux and colleagues could also demonstrate efficient endpoint ANCA titer analysis without serial dilution of samples using digital IIF [51], [52]. We and others have shown the usefulness of automated endpoint titer analysis for other autoantibodies such as ANA [20], [21], [43], [44]. Thus, automated IIF combining screening and confirmatory ANCA analysis simultaneously in one reaction environment appears to be a unique opportunity to replace the time-consuming classical two-tier ANCA testing by a one-step analysis.…”

Section: Discussionmentioning

confidence: 92%

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Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

et al. 2014

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Anti-neutrophil cytoplasmic antibodies (ANCA) are the serological hallmark of small vessel vasculitis, so called ANCA-associated vasculitis. The international consensus requires testing by indirect immunofluorescence (IIF) on human ethanol-fixed neutrophils (ethN) as screening followed by confirmation with enzyme-linked immunosorbent assays (ELISAs). This study evaluates the combination of cell- and microbead-based digital IIF analysis of ANCA in one reaction environment by the novel multiplexing CytoBead technology for simultaneous screening and confirmatory ANCA testing. Sera of 592 individuals including 118 patients with ANCA-associated vasculitis, 133 with rheumatoid arthritis, 49 with infectious diseases, 77 with inflammatory bowel syndrome, 20 with autoimmune liver diseases, 70 with primary sclerosing cholangitis and 125 blood donors were tested for cytoplasmic ANCA (C-ANCA) and perinuclear ANCA (P-ANCA) by classical IIF and ANCA to proteinase 3 (PR3) and myeloperoxidase (MPO) by ELISA. These findings were compared to respective ANCA results determined by automated multiplex CytoBead technology using ethN and antigen-coated microbeads for microbead immunoassays. There was a good agreement for PR3- and MPO-ANCA and a very good one for P-ANCA and C-ANCA by classical and multiplex analysis (Cohen's kappa [κ] = 0.775, 0.720, 0.876, 0.820, respectively). The differences between classical testing and CytoBead analysis were not significant for PR3-ANCA, P-ANCA, and C-ANCA (p<0.05, respectively). The prevalence of confirmed positive ANCA findings by classical testing (IIF and ELISA) compared with multiplex CytoBead analysis (IIF and microbead immunoassay positive) resulted in a very good agreement (κ = 0.831) with no significant difference of both methods (p = 0.735). Automated endpoint-ANCA titer detection in one dilution demonstrated a very good agreement with classical analysis requiring dilution of samples (κ = 0.985). Multiplexing by CytoBead technology can be employed for simultaneous screening and quantitative confirmation of ANCA. This novel technique provides fast and cost-effective ANCA analysis by automated digital IIF for the first time.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 92%

Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

et al. 2014

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“…Joachim Brugsch brought aboard Ernst Sch ü tte , Professor of Physiological Chemistry at the Free University of Berlin since 1951 (see also Table 2 in [22 ]). …”

Section: Prof Dr Med Dr Rer Nat Ernst Sch ü Ttementioning

confidence: 99%

“…But since it was the De Gruyter publishing house, and the request was expressed rather urgently, I have accepted the offer, not least to prevent worse from happening. ' The Zeitschrift f ü r Klinische Chemie was given its name [see also Table 1 in [22 ]] in analogy to the Zeitschrift f ü r Physiologische Chemie , published by the same publishing house in 1877 and founded by Felix Hoppe-Seyler (1825 -1895). Both journals changed their names and appearance several times, for reasons of substance and scientific policy but also for alphabetical reasons (based on its German title), e.g., so as not to be listed near the end of the ' Bibliographic Guide for Editors and Authors ' (published in 1974 by The American Chemical Society, Washington, DC).…”

Section: Prof Dr Med Dr Rer Nat Ernst Sch ü Ttementioning

confidence: 99%

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50th anniversary of Clinical Chemistry and Laboratory Medicine – a historical overview¹⁾

Körber¹,

Plebani

2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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In the early 1960s, Joachim Brugsch , one of the founders of Clinical Chemistry and Laboratory Medicine ( CCLM ) (then Zeitschrift f ü r Klinische Chemie ), had the idea to found a journal in the upcoming field of clinical chemistry. He approached Ernst Sch ü tte , who was associated with the De Gruyter publishing house through another journal, to participate, and Sch ü tte thus became the second founder of this Journal. The aim was to create a vehicle allowing the experts to express their opinions and raise their voices more clearly than they could in a journal that publishes only original experimental papers, a laborious and difficult, but important endeavor, as the profession of clinical chemistry was still in the early stages of development at this time. The first issue of this Journal was published in early 1963, and today, we are proud to celebrate the 50th anniversary of CCLM . This review describes the development of this Journal in light of the political situation of the time when it was founded, the situation of the publisher Walter De Gruyter after the erection of the Berlin Wall, and the development of clinical chemistry, and later on, laboratory medicine as a well-acknowledged discipline and profession.

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Circulating Cell-Free DNA for Molecular Diagnostics and Therapeutic Monitoring

Beaver

Park

2014

Genomic Applications in Pathology

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Preface: Happy 50th anniversary!

Cited by 20 publications

References 4 publications

Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

50th anniversary of Clinical Chemistry and Laboratory Medicine – a historical overview¹⁾

Circulating Cell-Free DNA for Molecular Diagnostics and Therapeutic Monitoring

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Preface: Happy 50th anniversary!

Cited by 20 publications

References 4 publications

Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

Simultaneous Automated Screening and Confirmatory Testing for Vasculitis-Specific ANCA

50th anniversary of Clinical Chemistry and Laboratory Medicine – a historical overview1)

Circulating Cell-Free DNA for Molecular Diagnostics and Therapeutic Monitoring

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Product

Resources

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50th anniversary of Clinical Chemistry and Laboratory Medicine – a historical overview¹⁾