Preferential expression of CD30 by human CD4 ⁺ T cells producing Th2‐type cytokines

Abstract

A large panel of human CD4+ T helper (Th) cell clones with established Th1, Th2, or Th0 profiles of cytokine secretion were examined for the expression of CD30, a member of the tumor necrosis factor receptor superfamily. Th1 clones expressed poor or no CD30 mRNA, and showed low or undetectable expression of both membrane and soluble CD30 (sCD30) protein, whereas Th2 clones showed both CD30 mRNA and membrane CD30 and released substantial amounts of sCD30, Th0 clones exhibited an intermediate pattern of CD30 expression and release. When T cells from the same donor were stimulated with three different antigens (purified protein derivative, PPD; Toxocara canis excretory/secretory antigen, TES; Lalium perenne group I, Lol p I), production of high concentrations of IFN‐γ, but not expression of CD30 or production of IL‐4 and IL‐5, were observed at any time after stimulation with PPD. In contrast, both CD30 expression and production of IL‐4 and IL‐5. but not of IFN‐γ, were concomitantly detectable in TES‐ and Lol p I‐reactive T cells, suggesting a temporal relationship between CD30 expression and beginning of Th2‐typc cytokine production. Finally, CD4+CD30+ T cells specific for Lol p I and inducible to production of Th2‐type cytokines were sorted out from the circulation of grass‐sensitive patients in concomitance with the onset of allergic symptoms during the seasonal exposure to grass pollen. Thus, CD30 expression appears to be associated with the differentiation/activation pathway of human T cells producing Th2‐type cytokines.—Del Prete, G., De Carli, M., Almerigogna, F., Daniel, C. K., D'Elios, M. M., Zancuoghi, G., Virante, F., Pizzolo, G., Romagnani, S. Preferential expression of CD30 by human CD4+ T cells producing Th2‐type cytokines, FASEB J. 9, 81‐86 (1995)

High serum level of soluble CD30 in acute primary HIV-1 infection

“…Previous in vitro findings demonstrated that HIV replication mainly occurs within CD4 + cells of Th2 and Th0-type [13], which preferentially express and release CD30 [4,10], and that HIV infection up-regulates CD30 cell expression and release [4,6]. In the light of the above observations, the elevated circulating levels of sCD30 during acute infection and their positive correlation with serum concentration of HIV antigen suggest the possibility that they reflect the high rate of in vivo occurring viral replication.…”

CD30 triggering by agonistic antibodies regulates CXCR4 expression and CXCL12 chemotactic activity in the cell line L540

“…[1][2][3][4] CD30, CD40, OX40, and CD95 at least have been involved in lymphoid differentiation and effector functions. 5 CD30, originally described as a marker of a number of lymphoma cells and reactive lymphoblasts, 6 is expressed by activated and memory T cells, [6][7][8][9] medullary thymocytes, 10,11 B cells, natural killer cells, and some nonhematologic cells. 12 Its expression by T cells is essentially dependent on activation involving IL-4 and CD28 signaling.…”

“…12 Its expression by T cells is essentially dependent on activation involving IL-4 and CD28 signaling. 9,13 The IL-4 requirement is probably the reason for the association of CD30 expression with preferential Th0-and Th2-type immune responses in vitro 8 and in vivo. [14][15][16] Stimulation of CD30 by CD153 (CD30 ligand) leads to nuclear mobilization of NF-B complexes 17 while inducing a signal-coupled depletion of TRAF2, which increases the cell sensitivity to TNFR1-dependent apoptosis.…”

“…10,20,21 High levels of soluble CD30 or TNF-␣ in the sera of patients infected with human immunodeficiency virus (HIV-1) at diagnosis have been shown to be an independent prognostic indicator of disease progression, 22,23 and the suggested role of CD30 in HIV-1 infection 15 has been linked to its ability to promote HIV-1 shedding through NF-B activation in infected cells. 24 The role of CD30 in HIV-1 infection, 15,22 the preferential association of CD30 with compartmentalized lymphoid subsets during differentiation 11 and effector responses, 16 and the evidence that Th1 versus Th2 or naive versus primed T cells are distinguished by specific patterns of CD30 8 and chemokine receptor expression 25,26 suggest some kind of relationship between CD30 signals and chemokine function. Recently, Muta et al 27 reported that CD30 signals increased amounts of CCR7 mRNA in the YT lymphoma cell line.…”

Autocrine Growth Regulation of CD30 Ligand in CD30-Expressing Reed-Sternberg Cells: Distinction Between Hodgkin's Disease and Anaplastic Large Cell Lymphoma

“…The CD30L-CD30 interaction, like TNF-R and CD95, is likely to be involved in cell death signaling and participates in negative selection of thymic T lymphocytes. In contrast, both CD30 and CD30L can be expressed by activated T cells, and the CD30-mediated signaling enhances proliferation and cytokine secretion by Th2 cells, and probably by Th1 and Th0 cells (Del Prete et al, 1995;Hamann et al, 1996;Mingari et al, 1996). For B cells, CD30L can stimulate its proliferation, antibody production, and secretion in a cytokine (eg, IL-4 and IL-5)-dependent manner (Shanebeck et al, 1995).…”