Preferential incorporation of &lt;I&gt;trans&lt;/I&gt;, &lt;I&gt;trans&lt;/I&gt;-conjugated linoleic acid isomers into the liver of suckling rats

Yang, Lin; Yeung, Sai Ying Venus; Huang, Yü; Wang, Han Qing; Chen, Zhenyu

doi:10.1079/bjnbjn2001516

Cited by 9 publications

(1 citation statement)

References 31 publications

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“…Then, the pups were euthanized by cervical dislocation. Their abdomens were opened, and milk clots within the stomach were weighed and stored at -78°C until analysis [12,23]. Dams were euthanized by cervical dislocation on day 10 of lactation.…”

Section: Methodsmentioning

confidence: 99%

A high dose of conjugated linoleic acid increases fatty liver and insulin resistance in lactating mice

Pang

Zhu

et al. 2019

PLoS ONE

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This study aimed to evaluate the effects of a high dose of conjugated linoleic acid (CLA) on lactating mice. In one experiment, Kunming mice were separated into four groups (n = 6 per group); the control (CON) group received 3.0% linoleic acid (LA) oil, the L-CLA group received a mixture of 2.0% LA and 1.0% CLA, the M-CLA group received a mixture of 1.0% LA and a 2.0% CLA, and the H-CLA group received 3.0% CLA. Feeding proceeded from day 4 to day 10 of lactation. In a second experiment, a CON group received 3.0% LA, and an H-CLA group received 3.0% CLA. Plasma parameters were analyzed for all groups, and insulin tolerance tests (ITTs) were conducted. CLA treatment did not affect dam weight but significantly decreased the food intake of dams during lactation. Furthermore, CLA decreased the weight of pups on day 10 of lactation; this effect was attributed to lower milk fat of dams in the CLA group than in those of the other groups. Relative to mice in the CON group, the mice in the H-CLA group displayed increased liver weight and liver triglyceride (TG) content as well as higher TG content and γ-glutamyl transferase (γ-GT) activity in the plasma. Moreover, high-dose CLA resulted in insulin resistance, possibly affecting the red blood cell (RBC) and hemoglobin (HCB) levels in the plasma. In conclusion, lactating mice receiving a high dose of CLA exhibited fatty liver, insulin resistance, and impaired lactation performance.

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Section: Methodsmentioning

confidence: 99%

A high dose of conjugated linoleic acid increases fatty liver and insulin resistance in lactating mice

Pang

Zhu

et al. 2019

PLoS ONE

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Distribution of polyunsaturated fatty acids including conjugated linoleic acids in total and subcellular fractions from healthy and cancerous parts of human kidneys

Hoffmann¹,

Blaudszun²,

Brunken³

et al. 2005

Lipids

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Differences in the FA composition of subcellular fractions from healthy and cancerous kidney tissues from the same patients were examined. Only minor differences in CLA content were found between the healthy and the cancerous tissue portions. Regarding the distribution pattern, CLA incorporation into nuclei and cytosol was significantly higher than incorporation into plasma membranes and mitochondria, which could be correlated to the neutral lipid content of these fractions. The subcellular distribution pattern of CLA was similar to that observed with monounsaturated FA but unlike that found with 18:2n-6, which underlines the different physiological properties of CLA and 18:2n-6. Because PUFA have been suggested to have an effect on cancer risk, the contents of n-3 and n-6 PUFA were determined in kidney and renal cell carcinoma (RCC). The 18:2n-6 content and delta5 desaturase activity were significantly lower, and the 18:3n-6, 20:3n-6, and 20:5n-3 contents and delta6 desaturase activity were significantly higher in RCC than in healthy renal tissue, indicating a changed PUFA metabolism in RCC. Previous research has suggested that CLA inhibits the elongation and desaturation of 18:2n-6 into 20:4n-6. In that case, one might speculate that a diet enriched in CLA would be a useful tool in preventing RCC. However, the involvement of CLA in preventing renal cancer could not be demonstrated definitively from the design of this experiment. Further understanding of the cause and/or consequence of the difference in FA metabolism may lead to a better understanding of RCC.

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Lipid class distribution of fatty acids including conjugated linoleic acids in healthy and cancerous parts of human kidneys

Hoffmann

Blaudszun

Brunken

et al. 2005

Lipids

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In this study the FA compositions of healthy and cancerous human renal tissues from the same patients are compared with special reference to the CLA and PUFA content. CLA was preferentially incorporated into neutral lipid compared with phospholipid classes. Its distribution profile was similar to that of monounsaturated FA, but unlike that found with 18:2n-6. Different incorporation patterns were found for individual CLA isomers. Comparing renal cell carcinoma (RCC) and healthy kidney, the total CLA content was significantly lower in the cholesterylester fraction and significantly higher in the PE and PS fractions from RCC. The most significant differences between healthy and cancerous renal tissue were in the content of t10,c12-CLA. Furthermore, the lipid class distributions of n-6 PUFA were determined, and several significant differences between RCC and healthy renal tissue were found. This is of interest, as it has been proposed that the anticarcinogenic properties of dietary CLA are associated with their interference in the metabolism of 20:4n-6. The involvement of CLA in preventing renal cancer cannot be definitively demonstrated from the design of this study, nor was it intended, but the complete determination of the FA composition of adjacent healthy and cancerous tissues may provide an insight if lipids are involved in this disease.

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Preferential incorporation of <I>trans</I>, <I>trans</I>-conjugated linoleic acid isomers into the liver of suckling rats

Cited by 9 publications

References 31 publications

A high dose of conjugated linoleic acid increases fatty liver and insulin resistance in lactating mice

A high dose of conjugated linoleic acid increases fatty liver and insulin resistance in lactating mice

Distribution of polyunsaturated fatty acids including conjugated linoleic acids in total and subcellular fractions from healthy and cancerous parts of human kidneys

Lipid class distribution of fatty acids including conjugated linoleic acids in healthy and cancerous parts of human kidneys

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