Pregnancy-associated major basic protein in amniotic fluid

Vernof, Karen K.; Ory, Steven J.; Gleich, Gerald J.

doi:10.1016/0165-0378(92)90039-7

Cited by 7 publications

(2 citation statements)

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“…MBP is a 117 residue cationic polypeptide and the proform (proMBP) has a highly glycosylated 88 residue N-terminal extension , making the proBMP far less cationic and abolishing the toxicity of MBP (Barker et al, 1988;Popken-Harris et al, 1995). ProMBP is also present in amniotic fluid, but in lower concentrations than in maternal serum (Vernof et al, 1992b). In pooled maternal serum proMBP is found complexed, partly 2:2 with pregnancy-associated plasma protein-A (PAPP-A) (Oxvig et al, 1993(Oxvig et al, , 1994b, and partly 2:2 with angiotensinogen or 2:2:2 with angiotensinogen and complement C3dg (Oxvig et al, 1995).…”

Section: Introductionmentioning

confidence: 97%

The proform of eosinophil major basic protein: a new maternal serum marker for Down syndrome

et al. 1999

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Section: Introductionmentioning

confidence: 97%

The proform of eosinophil major basic protein: a new maternal serum marker for Down syndrome

et al. 1999

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“…[92–94] The sources and physiologic role of these proteins remains to be determined. Recently, the proform of eosinophil major basic protein (ProMBP) has been demonstrated to exist in the serum from pregnant women (and also in non-pregnant women) complexed with a variable fraction of angiotensinogen.…”

Section: Commentsmentioning

confidence: 99%

The clinical significance of eosinophils in the amniotic fluid in preterm labor

Romero¹,

Kusanovic²,

Gomez³

et al. 2009

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective White blood cells are not traditionally considered to be normally present in amniotic fluid. This study was conducted after the observation that a patient with preterm labor and intact membranes had eosinophils as a predominant cell in the amniotic fluid, and had an episode of asthma during the index pregnancy. The goal of this study was to determine whether women presenting with preterm labor with eosinophils in the amniotic fluid had a different outcome than those without eosinophils as the predominant white blood cell in the amniotic cavity. Methods This retrospective case-control study included women who presented with preterm labor and intact membranes between 24 and 34 weeks of gestation. Patients underwent an amniocentesis shortly after admission for the assessment of the microbiologic status of the amniotic cavity and/or fetal lung maturity. Amniotic fluid was cultured for aerobic and anaerobic bacteria as well as genital mycoplasmas. Cytologic studies included amniotic fluid white blood cell count and differential, which was performed on cytocentrifuged specimens. Patients with microbial invasion of the amniotic cavity and/or a white blood cell count >20 cells/mm3 were excluded from the study. Cases were defined as women in whom the differential contained >20% of eosinophils. Controls were selected among women with an amniotic fluid eosinophil count ≤20% and matched for gestational age at amniocentesis. The analysis was conducted with non-parametric statistics. Results The study population consisted of 10 cases and 50 controls. Gestational age and cervical dilatation at admission were similar in both groups. Cases had a lower gestational age at delivery than controls [34.6 weeks, inter-quartile range (IQR) 32–37.3 weeks vs. 38.0 weeks, IQR 35–40 weeks, respectively; p=0.018]. The prevalence of preterm delivery ≤35 weeks was higher among patients who had >20% eosinophils than in the control group [50% (5/10) vs. 18% (9/50), respectively; p=0.029]. Similar results were observed for delivery at <37 weeks [Cases: 70% (7/10) vs. Controls: 36% (18/50); p=0.046]. Conclusions Women with preterm labor and intact membranes who have a large proportion of eosinophils in the amniotic fluid are at an increased risk for spontaneous preterm delivery. These patients may have had an episode of preterm labor related to a type I hypersensitivity reaction.

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