Pregnancy diagnosis in dairy goats using progesterone assay kits and oestrous observation

2001

An experiment was conducted on Awassi ewes, during the breeding season, to evaluate the effectiveness of intravaginal sponges for oestrous synchronisation and the effect of pregnant mare serum gonadotropin (PMSG) on twinning rate. Thirty intact ewes with an average live weight of 3.1 ± 9.1 kg were divided into 3 equal groups. Sponges containing 60 mg of medroxyprogesterone acetate (MAP) were inserted for 13 days in ewes in groups Tl and T2. Ewes in the latter group (T2) were injected intramuscularly, at the time of sponge withdrawal, with 500 i.u. of PMSG. The third group (C), received no treatment and served as a control. The mean interval to onset of oestrus was 2.0 days (range: 1.2-3.0 days) and 2.1 days (range: 1.0-3.9 days) after sponge withdrawal for ewes in groups Tl and T2, respectively (P > 0.05). In the control group (C), time of onset of oestrus was more variable (mean: 4.2 days, range: 1-14 days). Differences between the treated and the control animals in the time of onset of oestrus were significant (P < 0.05). The mean concentration of progesterone at sponge withdrawal was 0.16 and 0.08 and at mating was 0.11 and 0.10 nmol 1 -1 for groups T1 and T2, respectively. The treatments had no effect on duration of pregnancy, lamb birth weight, or lamb weaning weight. Using PMSG at sponge withdrawal increased twinning rate from 20% in groups T1 and C to 50% in group T2. It is concluded that intravaginal sponges (MAP) can be used for oestrous synchronisation in local Awassi ewes during the breeding season and that treatment with PMSG at the end of MAP treatment increases twinning rate.

Section: Discussionmentioning

confidence: 99%

Oestrous synchronisation and twinning rate of Syrian Awassi ewes treated with progestagen and PMSG during the breeding season

2001

“…Peripheral progesterone concentrations have been widely used as a vital tool in studying the reproductive physiology in animals (e.g., Zarkawi et al 1991;Engeland et al 1997;Zarkawi et al 1999). Daily analysis of progesterone level for a period of 35 days starting 4 days prior to the first injection through to 19 days after the second injection of PGF2a was very informative in assessing the stage of cyclicity, the effect on the corpus luteum, and the establishment of pregnancy.…”

Section: Progesterone Measurementsmentioning

confidence: 99%

The evaluation of two closes of prostaglandin F_2αanalogue, Prosolvin, for oestrous synchronisation of Syrian Awassi ewes

2000

An assessment was made of the effectiveness of two doses of Prosolvin, a synthetic prostaglandin F2 a , on oestrous synchronisation in local ewes during the breeding season. Thirty cycling Awassi ewes were divided equally into three groups. Ewes in group T10 and group T15 were injected intramuscularly twice with either 10 tng (T10) or 15 mg (T15) of Prosolvin, with an interval of 11 days. The third group received no treatment and served as a control group (C). Oestrus commenced in the ewes in group T10 at 83.3±42.9 hours, and in the ewes in the T15 group at 136.5 ± 109.9 hours, after the second injection of Prosolvin. In the control group (C), oestrus behaviour was more variable and commenced 251.2 ± 130.9 hours after introduction of the rams. In the time to onset of oestrus, the difference between the effects of the two doses was not significant (P > 0.05) but there was a significant difference (P < 0.05) between treated and untreated ewes. The mean serum progesterone level dropped sharply within 24 hours after the treatment, from 8.48 nmol I -1 to 0.52 nmol 1 -1 in the ewes in group T10, and from 7.95 nmol I -1 to 0.45 nmol I -1 in the group T15. The treatment had no effect on either the duration of pregnancy or birth weight. It could be concluded that it is possible to use the synthetic prostaglandin Prosolvin at doses of 10 or 15 mg for oestrous synchronisation in local Awassi ewes during the breeding season.

“…44 considered one of the most important parameters (Adnan et al 1991;Lopez-Sebastian et al 1991;Kadzere et al 1996;Engeland et al 1997;Kornalijnslijper et al 1997;Al-Merestani et al 1999;DeCastro et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Serum progesterone levels using radioimmunoassay during oestrous cycle of indigenous Damascus does

Soukouti

2001

An experiment was conducted on Damascus does to characterise the oestrous cycle and activity of the corpus luteum and to determine normal progesterone patterns during different phases of the oestrous cycle using radioimmunoassay. Fifteen 2-5-year-old indigenous cycling Damascus does were used during the normal breeding season for two consecutive oestrous cycles. Average length of the oestrous cycles was 21.2 ± 1.5 days (range: 19-26 days), consisting of 2.9 + 0.8 days (2-5 days) where progesterone level was low, averaging 0.69 ± 0.85 nmol l -1 (range: 0.00-3.08 nmol l -1 ), followed by a sharp increase in progesterone secretion indicating presence of active corpora lutea which lasted an average of 15.3 ± 1.4 days (range: 13-20 days), with an average progesterone level of 13.41 nmol l -1 (range: 3.26-27.98 nmol l -1 ), followed by a sharp decline in progesterone level, indicating inactive corpora lutea, which lasted an average of 3.1 ± 0.6 days (range: 2-5 days), with an average progesterone level of 0.68 nmol l -1 (range: 0.00-2.81 nmol l -1 ). Maximum progesterone level during the luteal phase averaged 18.67 ± 3.15nmol l -1 (range: 14.00-27.98 nmol l -1 ), which, on average, occurred on the 12.2th ± 3.2 day of the oestrous cycle. It was possible, for the first time, to characterise the oestrous cycle of the indigenous Damascus doe, and to determine normal progesterone patterns during different phases of the oestrous cycle. Based on the results reported above, it is also concluded that progesterone levels under 3.18 nmol l -1 were found on average during 2.9 days at the start of the oestrous cycle and during 3.1 days at the end of the oestrous cycle (follicular phase), and that progesterone levels above 3.18 nmol l -1 were found during the luteal phase lasting, on average, for 15.3 days.