Serum progesterone levels using radioimmunoassay during oestrous cycle of indigenous Damascus does

Abstract

An experiment was conducted on Damascus does to characterise the oestrous cycle and activity of the corpus luteum and to determine normal progesterone patterns during different phases of the oestrous cycle using radioimmunoassay. Fifteen 2-5-year-old indigenous cycling Damascus does were used during the normal breeding season for two consecutive oestrous cycles. Average length of the oestrous cycles was 21.2 ± 1.5 days (range: 19-26 days), consisting of 2.9 + 0.8 days (2-5 days) where progesterone level was low, averaging 0.69 ± 0.85 nmol l -1 (range: 0.00-3.08 nmol l -1 ), followed by a sharp increase in progesterone secretion indicating presence of active corpora lutea which lasted an average of 15.3 ± 1.4 days (range: 13-20 days), with an average progesterone level of 13.41 nmol l -1 (range: 3.26-27.98 nmol l -1 ), followed by a sharp decline in progesterone level, indicating inactive corpora lutea, which lasted an average of 3.1 ± 0.6 days (range: 2-5 days), with an average progesterone level of 0.68 nmol l -1 (range: 0.00-2.81 nmol l -1 ). Maximum progesterone level during the luteal phase averaged 18.67 ± 3.15nmol l -1 (range: 14.00-27.98 nmol l -1 ), which, on average, occurred on the 12.2th ± 3.2 day of the oestrous cycle. It was possible, for the first time, to characterise the oestrous cycle of the indigenous Damascus doe, and to determine normal progesterone patterns during different phases of the oestrous cycle. Based on the results reported above, it is also concluded that progesterone levels under 3.18 nmol l -1 were found on average during 2.9 days at the start of the oestrous cycle and during 3.1 days at the end of the oestrous cycle (follicular phase), and that progesterone levels above 3.18 nmol l -1 were found during the luteal phase lasting, on average, for 15.3 days.

Steroid hormone profile of Markhoz does (Iranian Angora) throughout estrous cycle and gestation period

“…The P4 concentrations remained at basal levels throughout the estrus as observed by others (Zarkawi and Soukouti 2001;Khadiga et al 2005). In Damascus goats, the P4 level during luteal phase ranged from 2.6 to 5.4 ng mL −1 (Khadiga et al 2005), which is comparable to the levels in Markhoz goats.…”

“…Assessment of progesterone (P4) and estradiol-17β (E2) levels during different physiological stages in animals is considered one of the most important parameters to determine their fertility status (Zarkawi and Soukouti 2001). Little information is available regarding changes in P4 and E2 levels during different reproductive stages in Markhoz goats compared to that in other farm animals (Riesenberg et al 2001).…”

Plasma Progesterone Analysis by a Time-Resolved Fluorescent Antibody Test to Monitor Estrous Cycles in Goats

“…previously. 20 In addition, the P 4 concentrations in a 1999 study 6 observed during the early luteal phase agree with the data reported in the current study. Moreover, the highest concentrations of P 4 were associated with the presence of corpora lutea.…”

“…The P 4 concentrations in cyclic goats decline and reach minimal concentrations during estrus, after which they gradually increase and reach maximal concentrations in the luteal phase. 6,10,20 In the current study, there was a close temporal relationship between the significant decrease in plasma P 4 concentrations measured by the TR-FAT and the manifestations of behavioral estrus. Mean plasma P 4 concentrations in this period were typical for the periestrous period.…”

Progesterone Profile of Goats Subjected to Oestrus Synchronization and its Relevance in Predicting Pregnancy Outcome

“…Thus the earliest diagnosis of pregnancy in Malabari goats in this study with the serum progesterone estimation was done on day 17 th post insemination. The progesterone level was low in non-pregnant goats from day 16 to 21 as reported by Zarkawi and Soukouti (2001), Medan et al (2004) and Titi et al (2008). Padilla-Rivas et al (2005) reported the detection of amniotic vesicle by day 19.5 ± 0.3 post insemination in Boer goats as the first indication of pregnancy.…”